CatLogenzyme kinetics catalog

Values CatLog read from this paper

The catalytic consequences of experimental evolution. Transition-state structure during catalysis by the evolved beta-galactosidases of Escherichia coli (ebg enzymes) changed by a single mutational…

PMID 2505746doi:10.1042/bj2600109Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 31 records (62 values) from this paper: ebg enzyme (evolved beta-galactosidase) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 31 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.2.1.23

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli1-(beta-D-galactopyranosyl)-3-bromopyridinium2.20.415.4 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli1-(beta-D-galactopyranosyl)-3-chloropyridinium2.30.366.4 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli1-(beta-D-galactopyranosyl)-3-methylpyridinium0.041.10.036 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli1-(beta-D-galactopyranosyl)pyridinium0.0961.60.060 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli2-(beta-D-galactopyranosyl)-4-bromoisoquinolinium0.780.352.2 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli2-(beta-D-galactopyranosyl)isoquinolinium0.0481.40.034 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli2-naphthyl beta-D-galactopyranoside3.910.080.39 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli2-nitrophenyl beta-D-galactopyranoside13.53.084.38 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli3,4-dinitrophenyl beta-D-galactopyranoside14.81.0314.4 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli3,5-dinitrophenyl beta-D-galactopyranoside15.11.2612.0 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli3-nitrophenyl beta-D-galactopyranoside14.37.12.0 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli4-bromophenyl beta-D-galactopyranoside7.517.150.44 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli4-cyanophenyl beta-D-galactopyranoside9.76.621.5 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli4-nitrophenyl beta-D-galactopyranoside15.82.995.28 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coliphenyl beta-D-galactopyranoside10.316.60.620 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli1-(beta-D-galactopyranosyl)-3,5-dimethylpyridinium0.0451.90.024 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli1-(beta-D-galactopyranosyl)-3-bromopyridinium5.60.3317 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli1-(beta-D-galactopyranosyl)-3-chloropyridinium5.30.3814 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli1-(beta-D-galactopyranosyl)-3-methylpyridinium0.041.50.027 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli1-(beta-D-galactopyranosyl)pyridinium0.213.10.068 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli2-(beta-D-galactopyranosyl)-4-bromoisoquinolinium8.30.4817 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli2-(beta-D-galactopyranosyl)isoquinolinium0.0720.790.091 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli2-naphthyl beta-D-galactopyranoside10.30.7314 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli3,4-dinitrophenyl beta-D-galactopyranoside11.60.89113.0 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli3,5-dinitrophenyl beta-D-galactopyranoside12.10.16772.5 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli3-methylphenyl beta-D-galactopyranoside5.71.713.3 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli3-nitrophenyl beta-D-galactopyranoside11.10.33233.4 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli4-bromophenyl beta-D-galactopyranoside8.81.028.6 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli4-cyanophenyl beta-D-galactopyranoside8.40.4519 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli4-nitrophenyl beta-D-galactopyranoside10.50.26839.2 calc.pH 7.5, 25 °COpen
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coliphenyl beta-D-galactopyranoside9.31.715.4 calc.pH 7.5, 25 °COpen