Values CatLog read from this paper
E461H-beta-galactosidase (Escherichia coli): altered divalent metal specificity and slow but reversible metal inactivation
PMID 7577931doi:10.1021/bi00041a022Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (40 values) from this paper: beta-galactosidase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 20 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| beta-galactosidase | Escherichia coli | o-nitrophenyl beta-D-galactopyranoside1 mM Mg2+ | 580 | 0.12 | 4.8 × 10³ calc. | pH 7, 25 °C | Open record: beta-galactosidase, o-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidase | Escherichia coli | o-nitrophenyl beta-D-galactopyranoside20 mM EDTA | 36 | 0.57 | 63 calc. | pH 7, 25 °C | Open record: beta-galactosidase, o-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidase | Escherichia coli | p-nitrophenyl beta-D-galactopyranoside1 mM Mg2+ | 98 | 0.034 | 2.9 × 10³ calc. | pH 7, 25 °C | Open record: beta-galactosidase, p-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidase | Escherichia coli | p-nitrophenyl beta-D-galactopyranosideEDTA (no Mg2+) | 13 | 0.12 | 110 calc. | pH 7, 25 °C | Open record: beta-galactosidase, p-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidasevariant E461H | Escherichia coli | o-nitrophenyl beta-D-galactopyranoside100 mM EDTA; no Mg2+ added | 44 | 0.15 | 290 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant E461H, o-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidasevariant E461H | Escherichia coli | o-nitrophenyl beta-D-galactopyranoside20 mM EDTA; no Mg2+ added | 50 | 0.17 | 290 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant E461H, o-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidasevariant E461H | Escherichia coli | o-nitrophenyl beta-D-galactopyranoside1 mM Mg2+ | 48 | 0.17 | 280 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant E461H, o-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidasevariant E461H | Escherichia coli | o-nitrophenyl beta-D-galactopyranoside5 mM Mg2+ | 37 | 0.29 | 130 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant E461H, o-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidasevariant E461H | Escherichia coli | o-nitrophenyl beta-D-galactopyranoside10 mM Mg2+ | 28 | 0.26 | 110 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant E461H, o-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidasevariant E461H | Escherichia coli | o-nitrophenyl beta-D-galactopyranoside20 mM Mg2+ | 20 | 0.38 | 53 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant E461H, o-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidasevariant E461H | Escherichia coli | o-nitrophenyl beta-D-galactopyranoside50 mM Mg2+ | 11 | 0.93 | 12 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant E461H, o-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidasevariant E461H | Escherichia coli | o-nitrophenyl beta-D-galactopyranoside100 mM Mg2+ | 5.7 | 1.8 | 3.2 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant E461H, o-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidasevariant E461H | Escherichia coli | p-nitrophenyl beta-D-galactopyranoside100 mM EDTA; no Mg2+ added | 3.8 | 0.13 | 29 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant E461H, p-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidasevariant E461H | Escherichia coli | p-nitrophenyl beta-D-galactopyranoside20 mM EDTA; no Mg2+ added | 4 | 0.15 | 27 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant E461H, p-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidasevariant E461H | Escherichia coli | p-nitrophenyl beta-D-galactopyranoside1 mM Mg2+ | 4 | 0.18 | 22 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant E461H, p-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidasevariant E461H | Escherichia coli | p-nitrophenyl beta-D-galactopyranoside5 mM Mg2+ | 2.2 | 0.22 | 10 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant E461H, p-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidasevariant E461H | Escherichia coli | p-nitrophenyl beta-D-galactopyranoside10 mM Mg2+ | 1.9 | 0.24 | 7.9 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant E461H, p-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidasevariant E461H | Escherichia coli | p-nitrophenyl beta-D-galactopyranoside20 mM Mg2+ | 1.6 | 0.4 | 4.0 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant E461H, p-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidasevariant E461H | Escherichia coli | p-nitrophenyl beta-D-galactopyranoside50 mM Mg2+ | 1.2 | 0.85 | 1.4 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant E461H, p-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidasevariant E461H | Escherichia coli | p-nitrophenyl beta-D-galactopyranoside100 mM Mg2+ | 1.5 | 2 | 0.75 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant E461H, p-nitrophenyl beta-D-galactopyranoside |