Values CatLog read from this paper
Catalytic consequences of experimental evolution: catalysis by a 'third-generation' evolvant of the second beta-galactosidase of Escherichia coli, ebgabcde, and by ebgabcd, a 'second-generation'…
PMID 8554546doi:10.1042/bj3120971Open in Browse
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CatLog read 22 records (42 values) from this paper: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 20 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde] | Escherichia coli | 2-nitrophenyl beta-D-galactopyranoside | 4.3 | 0.021 | 200 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde], 2-nitrophenyl beta-D-galactopyranoside |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde] | Escherichia coli | 3,4-dinitrophenyl beta-D-galactopyranoside | 3.9 | 0.028 | 140 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde], 3,4-dinitrophenyl beta-D-galactopyranoside |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde] | Escherichia coli | 3,4-dinitrophenyl beta-D-galactopyranoside | 8.6 | 0.033 | 260 calc. | pH 7.5, 37 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde], 3,4-dinitrophenyl beta-D-galactopyranoside |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde] | Escherichia coli | 3,5-dinitrophenyl beta-D-galactopyranoside | 4.5 | 0.023 | 200 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde], 3,5-dinitrophenyl beta-D-galactopyranoside |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde] | Escherichia coli | 3-nitrophenyl beta-D-galactopyranoside | 4 | 0.2 | 20 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde], 3-nitrophenyl beta-D-galactopyranoside |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde] | Escherichia coli | 4-bromophenyl beta-D-galactopyranoside | 4.4 | 0.082 | 54 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde], 4-bromophenyl beta-D-galactopyranoside |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde] | Escherichia coli | 4-cyanophenyl beta-D-galactopyranoside | 3.6 | 0.17 | 21 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde], 4-cyanophenyl beta-D-galactopyranoside |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde] | Escherichia coli | 4-nitrophenyl beta-D-galactopyranoside | 4.1 | 0.055 | 75 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde], 4-nitrophenyl beta-D-galactopyranoside |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde] | Escherichia coli | D-galactose | 13 | — | — | pH 7.5, 37 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde], D-galactose |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde] | Escherichia coli | phenyl beta-D-galactopyranoside | 2.3 | 0.33 | 7.0 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde], phenyl beta-D-galactopyranoside |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd] | Escherichia coli | 1-fluoro-D-galactopyranosyl fluoride | 0.114 | 0.085 | 1.3 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd], 1-fluoro-D-galactopyranosyl fluoride |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd] | Escherichia coli | 2-nitrophenyl beta-D-galactopyranoside | 11.3 | 0.087 | 130 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd], 2-nitrophenyl beta-D-galactopyranoside |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd] | Escherichia coli | 3,4-dinitrophenyl beta-D-galactopyranoside | 9.04 | 0.052 | 170 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd], 3,4-dinitrophenyl beta-D-galactopyranoside |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd] | Escherichia coli | 3,4-dinitrophenyl beta-D-galactopyranoside | 21.9 | 0.095 | 230 calc. | pH 7.5, 37 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd], 3,4-dinitrophenyl beta-D-galactopyranoside |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd] | Escherichia coli | 3,5-dinitrophenyl beta-D-galactopyranoside | 10.5 | 0.046 | 230 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd], 3,5-dinitrophenyl beta-D-galactopyranoside |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd] | Escherichia coli | 3-nitrophenyl beta-D-galactopyranoside | 10.8 | 0.12 | 90 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd], 3-nitrophenyl beta-D-galactopyranoside |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd] | Escherichia coli | 4-bromophenyl beta-D-galactopyranoside | 9.34 | 0.16 | 58 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd], 4-bromophenyl beta-D-galactopyranoside |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd] | Escherichia coli | 4-cyanophenyl beta-D-galactopyranoside | 11.1 | 0.1 | 110 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd], 4-cyanophenyl beta-D-galactopyranoside |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd] | Escherichia coli | 4-nitrophenyl beta-D-galactopyranoside | 10.1 | 0.11 | 92 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd], 4-nitrophenyl beta-D-galactopyranoside |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd] | Escherichia coli | beta-D-galactopyranosyl fluoride | 9 | 0.64 | 14 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd], beta-D-galactopyranosyl fluoride |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd] | Escherichia coli | D-galactose | 13 | — | — | pH 7.5, 37 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd], D-galactose |
| second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd] | Escherichia coli | phenyl beta-D-galactopyranoside | 7.05 | 0.35 | 20 calc. | pH 7.5, 25 °C | Open record: second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC products variant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd], phenyl beta-D-galactopyranoside |