Values CatLog read from this paper
Developing promiscuous glycosidases for glycoside synthesis: residues W433 and E432 in Sulfolobus solfataricus beta-glycosidase are important glucoside- and galactoside-specificity determinants
PMID 15846595doi:10.1002/cbic.200400341Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 51 records (143 values) from this paper: Sulfolobus solfataricus beta-glycosidase (SsβG) from Sulfolobus solfataricus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-methylumbelliferyl beta-D-fucopyranoside | 80 | 0.011 | 7.3 × 10³ | pH 6.5, 80 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-methylumbelliferyl beta-D-fucopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-methylumbelliferyl beta-D-galactopyranoside | 98 | 0.066 | 1.49 × 10³ | pH 6.5, 80 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-methylumbelliferyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-methylumbelliferyl beta-D-glucopyranoside | 140 | 0.046 | 2.9 × 10³ | pH 6.5, 80 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-methylumbelliferyl beta-D-glucopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-methylumbelliferyl beta-D-glucuronide | 0.81 | 1.3 | 0.6 | pH 6.5, 80 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-methylumbelliferyl beta-D-glucuronide |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-methylumbelliferyl beta-D-mannopyranoside | 1.8 | 0.036 | 50 | pH 6.5, 80 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-methylumbelliferyl beta-D-mannopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-methylumbelliferyl beta-D-xylopyranoside | 3.8 | 0.13 | 30 | pH 6.5, 80 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-methylumbelliferyl beta-D-xylopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-fucopyranoside | 5.16 | 0.21 | 42.64 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-fucopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosidebuffer; Table 2 | 7.2 | 0.57 | 12.74 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosideTable 3 | 24.66 | 5.06 | 4.873 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosideTable 3 | 19.73 | 2.81 | 7.021 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosideTable 3 | 17.58 | 2.9 | 6.061 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosideTable 3 | 12.24 | 0.67 | 18.38 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosideTable 3 | 11.55 | 0.58 | 19.87 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosideTable 3 | 11.38 | 0.49 | 23.04 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosideTable 3 | 8.32 | 5.27 | 1.579 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosideTable 3 | 18.08 | 6.33 | 2.857 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosideTable 3 | 8.83 | 5.12 | 1.724 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosideTable 3 | 18.08 | 2.2 | 8.22 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosideTable 3 | 13.91 | 0.49 | 28.33 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosideTable 3 | 14.67 | 1.84 | 7.973 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosideTable 3 | 12.77 | 0.73 | 17.55 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosiduronic acid | — | — | 0.06 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-galactopyranosiduronic acid |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-glucopyranoside | 4.35 | 0.15 | 29.80 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-glucopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-mannopyranoside | 2.65 | 24.2 | 0.109 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-mannopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG) | Sulfolobus solfataricus | 4-nitrophenyl beta-D-xylopyranoside | 3.95 | 5.1 | 0.774 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG), 4-nitrophenyl beta-D-xylopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432C | Sulfolobus solfataricus | 4-methylumbelliferyl beta-D-fucopyranoside | 18 | 0.34 | 53 | pH 6.5, 80 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant E432C, 4-methylumbelliferyl beta-D-fucopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432C | Sulfolobus solfataricus | 4-methylumbelliferyl beta-D-galactopyranoside | 5.4 | 0.47 | 11 | pH 6.5, 80 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant E432C, 4-methylumbelliferyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432C | Sulfolobus solfataricus | 4-methylumbelliferyl beta-D-glucopyranoside | 5.1 | 0.34 | 15 | pH 6.5, 80 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant E432C, 4-methylumbelliferyl beta-D-glucopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432C | Sulfolobus solfataricus | 4-methylumbelliferyl beta-D-mannopyranoside | 2.8 | 0.9 | 3.2 | pH 6.5, 80 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant E432C, 4-methylumbelliferyl beta-D-mannopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432C | Sulfolobus solfataricus | 4-methylumbelliferyl beta-D-xylopyranoside | 2.8 | 1.26 | 2.2 | pH 6.5, 80 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant E432C, 4-methylumbelliferyl beta-D-xylopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432C | Sulfolobus solfataricus | 4-nitrophenyl beta-D-fucopyranoside | — | — | 0.068 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant E432C, 4-nitrophenyl beta-D-fucopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432C | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosidebuffer; Table 2 | 0.047 | 0.81 | 0.058 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant E432C, 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432C | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosideTable 3 | 0.056 | 1.3 | 0.043 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant E432C, 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432C | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosideTable 3 | 0.067 | 1.65 | 0.04 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant E432C, 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432C | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosiduronic acid | 0.004 | 3.46 | 0.001 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant E432C, 4-nitrophenyl beta-D-galactopyranosiduronic acid |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432C | Sulfolobus solfataricus | 4-nitrophenyl beta-D-glucopyranoside | 0.018 | 0.51 | 0.036 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant E432C, 4-nitrophenyl beta-D-glucopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432C | Sulfolobus solfataricus | 4-nitrophenyl beta-D-mannopyranoside | — | — | 0.001 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant E432C, 4-nitrophenyl beta-D-mannopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432C | Sulfolobus solfataricus | 4-nitrophenyl beta-D-xylopyranoside | 0.028 | 13.7 | 0.002 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant E432C, 4-nitrophenyl beta-D-xylopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433C | Sulfolobus solfataricus | 4-methylumbelliferyl beta-D-fucopyranoside | 31 | 0.41 | 76 | pH 6.5, 80 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant W433C, 4-methylumbelliferyl beta-D-fucopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433C | Sulfolobus solfataricus | 4-methylumbelliferyl beta-D-galactopyranoside | 14.6 | 2.2 | 6.3 | pH 6.5, 80 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant W433C, 4-methylumbelliferyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433C | Sulfolobus solfataricus | 4-methylumbelliferyl beta-D-glucopyranoside | 33 | 1.61 | 20 | pH 6.5, 80 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant W433C, 4-methylumbelliferyl beta-D-glucopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433C | Sulfolobus solfataricus | 4-methylumbelliferyl beta-D-mannopyranoside | 0.92 | 0.18 | 5.1 | pH 6.5, 80 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant W433C, 4-methylumbelliferyl beta-D-mannopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433C | Sulfolobus solfataricus | 4-methylumbelliferyl beta-D-xylopyranoside | 1.5 | 0.59 | 2.5 | pH 6.5, 80 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant W433C, 4-methylumbelliferyl beta-D-xylopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433C | Sulfolobus solfataricus | 4-nitrophenyl beta-D-fucopyranoside | 9.43 | 18.6 | 0.506 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant W433C, 4-nitrophenyl beta-D-fucopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433C | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosidebuffer; Table 2 | 0.08 | 5.28 | 0.015 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant W433C, 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433C | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosideTable 3 | 0.08 | 18.3 | 0.004 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant W433C, 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433C | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosideTable 3 | 0.19 | 12.5 | 0.015 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant W433C, 4-nitrophenyl beta-D-galactopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433C | Sulfolobus solfataricus | 4-nitrophenyl beta-D-galactopyranosiduronic acid | 0.004 | 2.72 | 0.00129 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant W433C, 4-nitrophenyl beta-D-galactopyranosiduronic acid |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433C | Sulfolobus solfataricus | 4-nitrophenyl beta-D-glucopyranoside | 2.72 | 22.1 | 0.123 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant W433C, 4-nitrophenyl beta-D-glucopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433C | Sulfolobus solfataricus | 4-nitrophenyl beta-D-mannopyranoside | — | — | 0.005 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant W433C, 4-nitrophenyl beta-D-mannopyranoside |
| Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433C | Sulfolobus solfataricus | 4-nitrophenyl beta-D-xylopyranoside | — | — | 0.003 | pH 6.5, 45 °C | Open record: Sulfolobus solfataricus beta-glycosidase (SsβG) variant W433C, 4-nitrophenyl beta-D-xylopyranoside |