CatLogenzyme kinetics catalog

Values CatLog read from this paper

Determination of the roles of Glu-461 in beta-galactosidase (Escherichia coli) using site-specific mutagenesis

PMID 1969405doi:10.1016/s0021-9258(19)39391-3Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 42 records (50 values) from this paper: beta-galactosidase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.2.1.23

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
beta-galactosidaseEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay7500.126.2 × 10³ calc.pH 7, 25 °COpen
beta-galactosidaseEscherichia coli2-nitrophenyl beta-D-galactopyranosidewith 10 mM EDTA (enzyme pre-equilibrated 30-60 min)82.50.62130 calc.pH 7, 25 °COpen
beta-galactosidaseEscherichia coli4-nitrophenyl beta-D-galactopyranoside900.0382.4 × 10³ calc.pH 7, 25 °COpen
beta-galactosidasevariant E461DEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I0.1——pH 7, 25 °COpen
beta-galactosidasevariant E461DEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I—0.008—pH 7, 25 °COpen
beta-galactosidasevariant E461DEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I0.12——pH 7, 25 °COpen
beta-galactosidasevariant E461DEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I0.16——pH 7, 25 °COpen
beta-galactosidasevariant E461DEscherichia coli2-nitrophenyl beta-D-galactopyranosidewith 10 mM EDTA (enzyme pre-equilibrated 30-60 min)1.990.54.0 calc.pH 7, 25 °COpen
beta-galactosidasevariant E461DEscherichia coli4-nitrophenyl beta-D-galactopyranoside0.007——pH 7, 25 °COpen
beta-galactosidasevariant E461DEscherichia coli4-nitrophenyl beta-D-galactopyranoside—0.011—pH 7, 25 °COpen
beta-galactosidasevariant E461DEscherichia coli4-nitrophenyl beta-D-galactopyranoside10 mM sodium azide added as nucleophilic acceptor0.007——pH 7, 25 °COpen
beta-galactosidasevariant E461GEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I0.71——pH 7, 25 °COpen
beta-galactosidasevariant E461GEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I—0.008—pH 7, 25 °COpen
beta-galactosidasevariant E461GEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I18——pH 7, 25 °COpen
beta-galactosidasevariant E461GEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I29——pH 7, 25 °COpen
beta-galactosidasevariant E461GEscherichia coli2-nitrophenyl beta-D-galactopyranosidewith 10 mM EDTA (enzyme pre-equilibrated 30-60 min)0.1310.0245.5 calc.pH 7, 25 °COpen
beta-galactosidasevariant E461GEscherichia coli4-nitrophenyl beta-D-galactopyranoside0.103——pH 7, 25 °COpen
beta-galactosidasevariant E461GEscherichia coli4-nitrophenyl beta-D-galactopyranoside—0.014—pH 7, 25 °COpen
beta-galactosidasevariant E461GEscherichia coli4-nitrophenyl beta-D-galactopyranoside10 mM sodium azide added as nucleophilic acceptor0.105——pH 7, 25 °COpen
beta-galactosidasevariant E461HEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I42——pH 7, 25 °COpen
beta-galactosidasevariant E461HEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I—0.15—pH 7, 25 °COpen
beta-galactosidasevariant E461HEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I71——pH 7, 25 °COpen
beta-galactosidasevariant E461HEscherichia coli2-nitrophenyl beta-D-galactopyranosidewith 10 mM EDTA (enzyme pre-equilibrated 30-60 min)480.14340 calc.pH 7, 25 °COpen
beta-galactosidasevariant E461HEscherichia coli4-nitrophenyl beta-D-galactopyranoside2.1——pH 7, 25 °COpen
beta-galactosidasevariant E461HEscherichia coli4-nitrophenyl beta-D-galactopyranoside—0.074—pH 7, 25 °COpen
beta-galactosidasevariant E461HEscherichia coli4-nitrophenyl beta-D-galactopyranoside10 mM sodium azide added as nucleophilic acceptor2.2——pH 7, 25 °COpen
beta-galactosidasevariant E461KEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I0.2——pH 7, 25 °COpen
beta-galactosidasevariant E461KEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I—0.006—pH 7, 25 °COpen
beta-galactosidasevariant E461KEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I10——pH 7, 25 °COpen
beta-galactosidasevariant E461KEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I26——pH 7, 25 °COpen
beta-galactosidasevariant E461KEscherichia coli2-nitrophenyl beta-D-galactopyranosidewith 10 mM EDTA (enzyme pre-equilibrated 30-60 min)0.130.026.5 calc.pH 7, 25 °COpen
beta-galactosidasevariant E461KEscherichia coli4-nitrophenyl beta-D-galactopyranoside0.24——pH 7, 25 °COpen
beta-galactosidasevariant E461KEscherichia coli4-nitrophenyl beta-D-galactopyranoside—0.008—pH 7, 25 °COpen
beta-galactosidasevariant E461KEscherichia coli4-nitrophenyl beta-D-galactopyranoside10 mM sodium azide added as nucleophilic acceptor2.6——pH 7, 25 °COpen
beta-galactosidasevariant E461QEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I0.36——pH 7, 25 °COpen
beta-galactosidasevariant E461QEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I—0.001—pH 7, 25 °COpen
beta-galactosidasevariant E461QEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I22.5——pH 7, 25 °COpen
beta-galactosidasevariant E461QEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I52——pH 7, 25 °COpen
beta-galactosidasevariant E461QEscherichia coli2-nitrophenyl beta-D-galactopyranosidewith 10 mM EDTA (enzyme pre-equilibrated 30-60 min)0.0040.0320.12 calc.pH 7, 25 °COpen
beta-galactosidasevariant E461QEscherichia coli4-nitrophenyl beta-D-galactopyranoside0.34——pH 7, 25 °COpen
beta-galactosidasevariant E461QEscherichia coli4-nitrophenyl beta-D-galactopyranoside—0.003—pH 7, 25 °COpen
beta-galactosidasevariant E461QEscherichia coli4-nitrophenyl beta-D-galactopyranoside100 mM sodium azide added as nucleophilic acceptor19——pH 7, 25 °COpen