Values CatLog read from this paper
The catalytic consequences of experimental evolution. Studies on the subunit structure of the second (ebg) beta-galactosidase of Escherichia coli, and on catalysis by ebgab, an experimental evolvant…
PMID 1540130doi:10.1042/bj2820155Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (39 values) from this paper: ebg β-galactosidase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 19 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 1-(β-D-galactopyranosyl)pyridinium | 0.12 | 2.3 | 0.052 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, 1-(β-D-galactopyranosyl)pyridinium |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 2,4,6-trinitrophenyl 1-thio-β-D-galactopyranoside | 2.8 | 0.26 | 11 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, 2,4,6-trinitrophenyl 1-thio-β-D-galactopyranoside |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 2,4-dinitrophenyl 1-thio-β-D-galactopyranoside | 0.56 | 0.16 | 3.5 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, 2,4-dinitrophenyl 1-thio-β-D-galactopyranoside |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 2-(β-D-galactopyranosyl)isoquinolinium | 0.11 | 2.3 | 0.048 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, 2-(β-D-galactopyranosyl)isoquinolinium |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 2-naphthyl β-D-galactopyranoside | 1.5 | 0.18 | 8.3 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, 2-naphthyl β-D-galactopyranoside |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 2-nitrophenyl β-D-galactopyranoside | 4 | 0.056 | 71 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, 2-nitrophenyl β-D-galactopyranoside |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 3,4-dinitrophenyl β-D-galactopyranoside | 3.3 | 0.047 | 70 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, 3,4-dinitrophenyl β-D-galactopyranoside |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 3,4-dinitrophenyl β-D-galactopyranoside | 10.2 | 0.049 | 210 calc. | pH 7.5, 37 °C | Open record: ebg β-galactosidase variant D92N/W977C, 3,4-dinitrophenyl β-D-galactopyranoside |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 3,5-dinitrophenyl β-D-galactopyranoside | 3.9 | 0.082 | 48 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, 3,5-dinitrophenyl β-D-galactopyranoside |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 3-bromo-1-(β-D-galactopyranosyl)pyridinium | 4 | 0.38 | 11 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, 3-bromo-1-(β-D-galactopyranosyl)pyridinium |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 3-chloro-1-(β-D-galactopyranosyl)pyridinium | 3.1 | 0.34 | 9.1 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, 3-chloro-1-(β-D-galactopyranosyl)pyridinium |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 3-methyl-1-(β-D-galactopyranosyl)pyridinium | 0.022 | 0.7 | 0.031 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, 3-methyl-1-(β-D-galactopyranosyl)pyridinium |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 3-methylphenyl β-D-galactopyranoside | 1.5 | 0.32 | 4.7 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, 3-methylphenyl β-D-galactopyranoside |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 3-nitrophenyl β-D-galactopyranoside | 4.4 | 0.28 | 16 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, 3-nitrophenyl β-D-galactopyranoside |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 4-bromo-2-(β-D-galactopyranosyl)isoquinolinium | 3 | 0.22 | 14 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, 4-bromo-2-(β-D-galactopyranosyl)isoquinolinium |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 4-bromophenyl β-D-galactopyranoside | 2.7 | 0.21 | 13 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, 4-bromophenyl β-D-galactopyranoside |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 4-cyanophenyl β-D-galactopyranoside | 1.7 | 0.21 | 8.1 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, 4-cyanophenyl β-D-galactopyranoside |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | 4-nitrophenyl β-D-galactopyranoside | 3.1 | 0.1 | 31 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, 4-nitrophenyl β-D-galactopyranoside |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | D-galactose | 16 | — | — | pH 7.5, 37 °C | Open record: ebg β-galactosidase variant D92N/W977C, D-galactose |
| ebg β-galactosidasevariant D92N/W977C | Escherichia coli | phenyl β-D-galactopyranoside | 1.2 | 0.64 | 1.9 calc. | pH 7.5, 25 °C | Open record: ebg β-galactosidase variant D92N/W977C, phenyl β-D-galactopyranoside |