Values CatLog read from this paper
Biochemical characterization of a beta-galactosidase with a low temperature optimum obtained from an Antarctic arthrobacter isolate
PMID 12949099doi:10.1128/jb.185.18.5473-5482.2003Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (26 values) from this paper: BgaS (beta-galactosidase), LacZ (beta-galactosidase) from Arthrobacter sp., Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| BgaS (beta-galactosidase) | Arthrobacter sp. | lactose | 4.7 | 19.4 | 0.27 | 5 °C | Open record: BgaS (beta-galactosidase), lactose |
| BgaS (beta-galactosidase) | Arthrobacter sp. | lactose | 5.2 | 11.5 | 0.53 | 18 °C | Open record: BgaS (beta-galactosidase), lactose |
| BgaS (beta-galactosidase) | Arthrobacter sp. | o-nitrophenyl-beta-D-galactopyranoside | 43 | 0.7 | 61.4 | 5 °C | Open record: BgaS (beta-galactosidase), o-nitrophenyl-beta-D-galactopyranoside |
| BgaS (beta-galactosidase) | Arthrobacter sp. | o-nitrophenyl-beta-D-galactopyranoside | 57.2 | 0.7 | 81.7 | 10 °C | Open record: BgaS (beta-galactosidase), o-nitrophenyl-beta-D-galactopyranoside |
| BgaS (beta-galactosidase) | Arthrobacter sp. | o-nitrophenyl-beta-D-galactopyranoside | 100 | 0.8 | 116.3 | 18 °C | Open record: BgaS (beta-galactosidase), o-nitrophenyl-beta-D-galactopyranoside |
| BgaS (beta-galactosidase) | Arthrobacter sp. | o-nitrophenyl-beta-D-galactopyranosideN-terminal six-histidine fusion (H-BgaS); pET28a | — | 0.6 | 183.6 | 20 °C | Open record: BgaS (beta-galactosidase), o-nitrophenyl-beta-D-galactopyranoside |
| BgaS (beta-galactosidase) | Arthrobacter sp. | o-nitrophenyl-beta-D-galactopyranoside | 583.5 | 0.3 | 1823 | 20 °C | Open record: BgaS (beta-galactosidase), o-nitrophenyl-beta-D-galactopyranoside |
| LacZ (beta-galactosidase) | Escherichia coli | o-nitrophenyl-beta-D-galactopyranosideN-terminal six-histidine fusion (H-LacZ); pET28a | 31.7 | 0.2 | 166 | 20 °C | Open record: LacZ (beta-galactosidase), o-nitrophenyl-beta-D-galactopyranoside |
| LacZ (beta-galactosidase) | Escherichia coli | o-nitrophenyl-beta-D-galactopyranoside | 219.6 | 0.2 | 1098 | 20 °C | Open record: LacZ (beta-galactosidase), o-nitrophenyl-beta-D-galactopyranoside |