Values CatLog read from this paper
His-357 of beta-galactosidase (Escherichia coli) interacts with the C3 hydroxyl in the transition state and helps to mediate catalysis
PMID 9665715doi:10.1021/bi972796tOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 27 records (77 values) from this paper: beta-galactosidase (LacZ) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| beta-galactosidase (LacZ) | Escherichia coli | 2,3-dinitrophenyl beta-D-galactopyranoside | 1.19 × 10³ | 0.15 | 7.93 × 10³ | pH 7, 25 °C | Open record: beta-galactosidase (LacZ), 2,3-dinitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ) | Escherichia coli | 2,5-dinitrophenyl beta-D-galactopyranoside | 130 | 0.41 | 315 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ), 2,5-dinitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ) | Escherichia coli | 3,4-dinitrophenyl beta-D-galactopyranoside | 150 | 0.16 | 915 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ), 3,4-dinitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ) | Escherichia coli | o-nitrophenyl beta-D-galactopyranosideTES assay buffer: 30 mM TES; 1 mM MgSO4 | 620 | 0.14 | 4.43 × 10³ | pH 7, 25 °C | Open record: beta-galactosidase (LacZ), o-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ) | Escherichia coli | o-nitrophenyl beta-D-galactopyranoside30 mM TES; no added Mg2+ (10 mM EDTA instead of 1 mM MgSO4) | 39 | — | — | pH 7, 25 °C | Open record: beta-galactosidase (LacZ), o-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ) | Escherichia coli | p-nitrophenyl beta-D-galactopyranoside | 90 | 0.041 | 2.2 × 10³ | pH 7, 25 °C | Open record: beta-galactosidase (LacZ), p-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357D | Escherichia coli | 2,3-dinitrophenyl beta-D-galactopyranoside | 104 | 0.079 | 1.31 × 10³ | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357D, 2,3-dinitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357D | Escherichia coli | 2,5-dinitrophenyl beta-D-galactopyranoside | 210 | 0.23 | 920 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357D, 2,5-dinitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357D | Escherichia coli | 3,4-dinitrophenyl beta-D-galactopyranoside | 4.3 | 0.009 | 480 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357D, 3,4-dinitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357D | Escherichia coli | o-nitrophenyl beta-D-galactopyranoside | 7.8 | 0.13 | 59 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357D, o-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357D | Escherichia coli | p-nitrophenyl beta-D-galactopyranoside | 0.1 | 0.003 | 33 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357D, p-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357F | Escherichia coli | 2,3-dinitrophenyl beta-D-galactopyranoside | 52 | 0.83 | 63 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357F, 2,3-dinitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357F | Escherichia coli | 2,5-dinitrophenyl beta-D-galactopyranoside | 320 | 0.49 | 660 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357F, 2,5-dinitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357F | Escherichia coli | 3,4-dinitrophenyl beta-D-galactopyranoside | 11.4 | 0.073 | 156 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357F, 3,4-dinitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357F | Escherichia coli | o-nitrophenyl beta-D-galactopyranosideTES assay buffer: 30 mM TES; 1 mM MgSO4 | 16 | 1.25 | 13 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357F, o-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357F | Escherichia coli | o-nitrophenyl beta-D-galactopyranoside30 mM TES; no added Mg2+ (10 mM EDTA instead of 1 mM MgSO4) | 1.1 | — | — | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357F, o-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357F | Escherichia coli | p-nitrophenyl beta-D-galactopyranoside | 2.7 | 0.083 | 32.5 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357F, p-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357L | Escherichia coli | 2,3-dinitrophenyl beta-D-galactopyranoside | 13.1 | 0.11 | 120 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357L, 2,3-dinitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357L | Escherichia coli | 2,5-dinitrophenyl beta-D-galactopyranoside | 10.5 | 0.25 | 42 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357L, 2,5-dinitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357L | Escherichia coli | 3,4-dinitrophenyl beta-D-galactopyranoside | 4.5 | 0.061 | 74 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357L, 3,4-dinitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357L | Escherichia coli | o-nitrophenyl beta-D-galactopyranoside | 5.1 | 0.49 | 11 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357L, o-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357L | Escherichia coli | p-nitrophenyl beta-D-galactopyranoside | 0.4 | 0.003 | 12 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357L, p-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357N | Escherichia coli | 2,3-dinitrophenyl beta-D-galactopyranoside | 1.3 × 10³ | 0.2 | 6.5 × 10³ | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357N, 2,3-dinitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357N | Escherichia coli | 2,5-dinitrophenyl beta-D-galactopyranoside | 1.89 × 10³ | 0.57 | 3.33 × 10³ | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357N, 2,5-dinitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357N | Escherichia coli | 3,4-dinitrophenyl beta-D-galactopyranoside | 70 | 0.014 | 5.01 × 10³ | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357N, 3,4-dinitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357N | Escherichia coli | o-nitrophenyl beta-D-galactopyranoside | 64 | 0.21 | 290 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357N, o-nitrophenyl beta-D-galactopyranoside |
| beta-galactosidase (LacZ)variant H357N | Escherichia coli | p-nitrophenyl beta-D-galactopyranoside | 0.8 | 0.006 | 130 | pH 7, 25 °C | Open record: beta-galactosidase (LacZ) variant H357N, p-nitrophenyl beta-D-galactopyranoside |