Values CatLog read from this paper
Trp-999 of beta-galactosidase (Escherichia coli) is a key residue for binding, catalysis, and synthesis of allolactose, the natural lac operon inducer
PMID 12578395doi:10.1021/bi0270642Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (20 values) from this paper: beta-galactosidase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| beta-galactosidase | Escherichia coli | ortho-nitrophenyl-β-D-galactopyranoside | 600 | 0.12 | 5.0 × 10³ calc. | pH 7, 25 °C | Open record: beta-galactosidase, ortho-nitrophenyl-β-D-galactopyranoside |
| beta-galactosidase | Escherichia coli | para-nitrophenyl-β-D-galactopyranoside | 90 | 0.04 | 2.2 × 10³ calc. | pH 7, 25 °C | Open record: beta-galactosidase, para-nitrophenyl-β-D-galactopyranoside |
| beta-galactosidasevariant W999F | Escherichia coli | ortho-nitrophenyl-β-D-galactopyranoside | 54 | 0.26 | 210 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant W999F, ortho-nitrophenyl-β-D-galactopyranoside |
| beta-galactosidasevariant W999F | Escherichia coli | para-nitrophenyl-β-D-galactopyranoside | 67 | 0.43 | 160 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant W999F, para-nitrophenyl-β-D-galactopyranoside |
| beta-galactosidasevariant W999G | Escherichia coli | ortho-nitrophenyl-β-D-galactopyranoside | 48 | 0.31 | 150 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant W999G, ortho-nitrophenyl-β-D-galactopyranoside |
| beta-galactosidasevariant W999G | Escherichia coli | para-nitrophenyl-β-D-galactopyranoside | 52 | 0.52 | 100 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant W999G, para-nitrophenyl-β-D-galactopyranoside |
| beta-galactosidasevariant W999L | Escherichia coli | ortho-nitrophenyl-β-D-galactopyranoside | 20 | 3.3 | 6.1 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant W999L, ortho-nitrophenyl-β-D-galactopyranoside |
| beta-galactosidasevariant W999L | Escherichia coli | para-nitrophenyl-β-D-galactopyranoside | 28 | 2.1 | 13 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant W999L, para-nitrophenyl-β-D-galactopyranoside |
| beta-galactosidasevariant W999Y | Escherichia coli | ortho-nitrophenyl-β-D-galactopyranoside | 56 | 2.1 | 27 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant W999Y, ortho-nitrophenyl-β-D-galactopyranoside |
| beta-galactosidasevariant W999Y | Escherichia coli | para-nitrophenyl-β-D-galactopyranoside | 65 | 1.6 | 41 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant W999Y, para-nitrophenyl-β-D-galactopyranoside |