Values CatLog read from this paper

DNA family shuffling of hyperthermostable beta-glycosidases

PMID 12164784doi:10.1042/bj20020726Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 20 records (60 values) from this paper: CelB/LacS hybrid β-glycosidase, CelB, LacS from Pyrococcus furiosus, Sulfolobus solfataricus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.2.1.23

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
CelB	Pyrococcus furiosus	cellobiose	37.6	3.2	11.8	pH 5, 70 °C	Open record: CelB, cellobiose
CelB	Pyrococcus furiosus	lactose	286	59.8	4.78	pH 5, 70 °C	Open record: CelB, lactose
CelB	Pyrococcus furiosus	p-nitrophenyl-β-D-galactopyranoside	298	2.6	115	pH 5, 70 °C	Open record: CelB, p-nitrophenyl-β-D-galactopyranoside
CelB	Pyrococcus furiosus	p-nitrophenyl-β-D-glucopyranoside	206	0.8	258	pH 5, 70 °C	Open record: CelB, p-nitrophenyl-β-D-glucopyranoside
CelB/LacS hybrid β-glycosidasevariant hybrid 11	—	cellobiose	42.5	11.8	3.6	pH 5, 70 °C	Open record: CelB/LacS hybrid β-glycosidase variant hybrid 11, cellobiose
CelB/LacS hybrid β-glycosidasevariant hybrid 11	—	lactose	442	71.8	6.16	pH 5, 70 °C	Open record: CelB/LacS hybrid β-glycosidase variant hybrid 11, lactose
CelB/LacS hybrid β-glycosidasevariant hybrid 11	—	p-nitrophenyl-β-D-galactopyranoside	327	3.99	82	pH 5, 70 °C	Open record: CelB/LacS hybrid β-glycosidase variant hybrid 11, p-nitrophenyl-β-D-galactopyranoside
CelB/LacS hybrid β-glycosidasevariant hybrid 11	—	p-nitrophenyl-β-D-glucopyranoside	235	0.71	331	pH 5, 70 °C	Open record: CelB/LacS hybrid β-glycosidase variant hybrid 11, p-nitrophenyl-β-D-glucopyranoside
CelB/LacS hybrid β-glycosidasevariant hybrid 18	—	cellobiose	264	4.6	57.4	pH 5, 70 °C	Open record: CelB/LacS hybrid β-glycosidase variant hybrid 18, cellobiose
CelB/LacS hybrid β-glycosidasevariant hybrid 18	—	lactose	1022	88.6	11.5	pH 5, 70 °C	Open record: CelB/LacS hybrid β-glycosidase variant hybrid 18, lactose
CelB/LacS hybrid β-glycosidasevariant hybrid 18	—	p-nitrophenyl-β-D-galactopyranoside	743	5.63	132	pH 5, 70 °C	Open record: CelB/LacS hybrid β-glycosidase variant hybrid 18, p-nitrophenyl-β-D-galactopyranoside
CelB/LacS hybrid β-glycosidasevariant hybrid 18	—	p-nitrophenyl-β-D-glucopyranoside	440	0.58	759	pH 5, 70 °C	Open record: CelB/LacS hybrid β-glycosidase variant hybrid 18, p-nitrophenyl-β-D-glucopyranoside
CelB/LacS hybrid β-glycosidasevariant hybrid 20	—	cellobiose	60.1	12.1	4.97	pH 5, 70 °C	Open record: CelB/LacS hybrid β-glycosidase variant hybrid 20, cellobiose
CelB/LacS hybrid β-glycosidasevariant hybrid 20	—	lactose	447	59.5	7.51	pH 5, 70 °C	Open record: CelB/LacS hybrid β-glycosidase variant hybrid 20, lactose
CelB/LacS hybrid β-glycosidasevariant hybrid 20	—	p-nitrophenyl-β-D-galactopyranoside	412	6.58	62.6	pH 5, 70 °C	Open record: CelB/LacS hybrid β-glycosidase variant hybrid 20, p-nitrophenyl-β-D-galactopyranoside
CelB/LacS hybrid β-glycosidasevariant hybrid 20	—	p-nitrophenyl-β-D-glucopyranoside	276	0.75	368	pH 5, 70 °C	Open record: CelB/LacS hybrid β-glycosidase variant hybrid 20, p-nitrophenyl-β-D-glucopyranoside
LacS	Sulfolobus solfataricus	cellobiose	23.2	13.5	1.72	pH 5, 70 °C	Open record: LacS, cellobiose
LacS	Sulfolobus solfataricus	lactose	121	37.9	3.19	pH 5, 70 °C	Open record: LacS, lactose
LacS	Sulfolobus solfataricus	p-nitrophenyl-β-D-galactopyranoside	165	1.57	105	pH 5, 70 °C	Open record: LacS, p-nitrophenyl-β-D-galactopyranoside
LacS	Sulfolobus solfataricus	p-nitrophenyl-β-D-glucopyranoside	45	0.12	375	pH 5, 70 °C	Open record: LacS, p-nitrophenyl-β-D-glucopyranoside