CatLogenzyme kinetics catalog

Values CatLog read from this paper

Thymidylate kinase of Mycobacterium tuberculosis: a chimera sharing properties common to eukaryotic and bacterial enzymes

PMID 11369858doi:10.1110/ps.45701Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 9 records (10 values) from this paper: thymidylate kinase (TMPKmt) from Mycobacterium tuberculosis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.

EC 2.7.4.9

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
thymidylate kinase (TMPKmt)Mycobacterium tuberculosis2'-deoxyuridine 5'-monophosphate—2.1—pH 7.4, 30 °COpen
thymidylate kinase (TMPKmt)Mycobacterium tuberculosis3'-fluoro-3'-deoxythymidine 5'-monophosphate—0.03—pH 7.4, 30 °COpen
thymidylate kinase (TMPKmt)Mycobacterium tuberculosis5-bromo-2'-deoxyuridine 5'-monophosphate—0.03—pH 7.4, 30 °COpen
thymidylate kinase (TMPKmt)Mycobacterium tuberculosis5-fluoro-2'-deoxyuridine 5'-monophosphate—0.42—pH 7.4, 30 °COpen
thymidylate kinase (TMPKmt)Mycobacterium tuberculosis5-iodo-2'-deoxyuridine 5'-monophosphate—0.14—pH 7.4, 30 °COpen
thymidylate kinase (TMPKmt)Mycobacterium tuberculosisadenosine 5'-triphosphate—0.1—pH 7.4, 30 °COpen
thymidylate kinase (TMPKmt)Mycobacterium tuberculosisdehydro-TMP—0.14—pH 7.4, 30 °COpen
thymidylate kinase (TMPKmt)Mycobacterium tuberculosisthymidine 5'-monophosphateATP fixed at 0.2; 0.34.50.00451.0 × 10³ calc.pH 7.4, 30 °COpen
thymidylate kinase (TMPKmt)Mycobacterium tuberculosisthymidine 5'-monophosphateATP fixed at 0.5 mM; NMP varied 0.05-3.6 mM—0.04—pH 7.4, 30 °COpen