Values CatLog read from this paper
Folate activation and catalysis in methylenetetrahydrofolate reductase from Escherichia coli: roles for aspartate 120 and glutamate 28
PMID 11371182doi:10.1021/bi002790vOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 4 records (8 values) from this paper: methylenetetrahydrofolate reductase (MTHFR) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| methylenetetrahydrofolate reductase (MTHFR)variant D120N | Escherichia coli | 5,10-methylenetetrahydrofolate | 0.057 | 0.027 | 2.1 calc. | pH 7.2, 25 °C | Open record: methylenetetrahydrofolate reductase (MTHFR) variant D120N, 5,10-methylenetetrahydrofolate |
| methylenetetrahydrofolate reductase (MTHFR)variant D120N | Escherichia coli | 5-methyltetrahydrofolate | 0.087 | 0.106 | 0.82 calc. | pH 7.2, 25 °C | Open record: methylenetetrahydrofolate reductase (MTHFR) variant D120N, 5-methyltetrahydrofolate |
| methylenetetrahydrofolate reductase (MTHFR)variant D120N | Escherichia coli | NADH | 54 | 0.017 | 3.2 × 10³ calc. | pH 7.2, 25 °C | Open record: methylenetetrahydrofolate reductase (MTHFR) variant D120N, NADH |
| methylenetetrahydrofolate reductase (MTHFR)variant E28Q | Escherichia coli | NADH | 0.2 | 0.08 | 2.5 calc. | pH 7.2, 25 °C | Open record: methylenetetrahydrofolate reductase (MTHFR) variant E28Q, NADH |