CatLogenzyme kinetics catalog

EC 1.5.1.54: methylenetetrahydrofolate reductase (NADH)

OxidoreductasesOpen in Browse

CatLog read 23 records for EC 1.5.1.54 from 3 papers, for 1 organism. Names in these papers: methylenetetrahydrofolate reductase (MTHFR), methylenetetrahydrofolate reductase.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 11 records for EC 1.5.1.54 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.5.1.54 in Browse.

Papers

PaperOrganismsRecords
A role for glutamine 183 in the folate oxidative half-reaction of methylenetetrahydrofolate reductase from Escherichia coliEscherichia coli13
Functional role for the conformationally mobile phenylalanine 223 in the reaction of methylenetetrahydrofolate reductase from Escherichia coliEscherichia coli6
Folate activation and catalysis in methylenetetrahydrofolate reductase from Escherichia coli: roles for aspartate 120 and glutamate 28Escherichia coli4

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
methylenetetrahydrofolate reductasevariant F223AEscherichia coli(6R)-5,10-methylenetetrahydrofolate2.90.09331 calc.pH 7.2, 25 °CPMID 19610625Open
methylenetetrahydrofolate reductasevariant F223AEscherichia coliNADHNADH-menadione oxidoreductase assay (aerobic)220.58538 calc.pH 7.2, 25 °CPMID 19610625Open
methylenetetrahydrofolate reductasevariant F223AEscherichia coliNADHNADH-CH2-H4folate oxidoreductase assay (anaerobic)—0.14—pH 7.2, 25 °CPMID 19610625Open
methylenetetrahydrofolate reductasevariant F223LEscherichia coli(6R)-5,10-methylenetetrahydrofolate140.0081.8 × 10³ calc.pH 7.2, 25 °CPMID 19610625Open
methylenetetrahydrofolate reductasevariant F223LEscherichia coliNADHNADH-menadione oxidoreductase assay (aerobic)310.4766 calc.pH 7.2, 25 °CPMID 19610625Open
methylenetetrahydrofolate reductasevariant F223LEscherichia coliNADHNADH-CH2-H4folate oxidoreductase assay (anaerobic)—0.236—pH 7.2, 25 °CPMID 19610625Open
methylenetetrahydrofolate reductase (MTHFR)Escherichia coli(6S)-5-methyltetrahydrofolate0.470.0316 calc.pH 7.2, 4 °CPMID 29407039Open
methylenetetrahydrofolate reductase (MTHFR)Escherichia colimenadione—2.1 × 10⁻⁴—pH 7.2, 4 °CPMID 29407039Open
methylenetetrahydrofolate reductase (MTHFR)Escherichia coliNADH18.20.025730 calc.pH 7.2, 4 °CPMID 29407039Open
methylenetetrahydrofolate reductase (MTHFR)variant D120NEscherichia coli5,10-methylenetetrahydrofolate0.0570.0272.1 calc.pH 7.2, 25 °CPMID 11371182Open
methylenetetrahydrofolate reductase (MTHFR)variant D120NEscherichia coli5-methyltetrahydrofolate0.0870.1060.82 calc.pH 7.2, 25 °CPMID 11371182Open
methylenetetrahydrofolate reductase (MTHFR)variant D120NEscherichia coliNADH540.0173.2 × 10³ calc.pH 7.2, 25 °CPMID 11371182Open
methylenetetrahydrofolate reductase (MTHFR)variant E28QEscherichia coliNADH0.20.082.5 calc.pH 7.2, 25 °CPMID 11371182Open
methylenetetrahydrofolate reductase (MTHFR)variant Q183AEscherichia coli(6R)-5,10-methylenetetrahydrofolate0.290.1042.8 calc.pH 7.2, 4 °CPMID 29407039Open
methylenetetrahydrofolate reductase (MTHFR)variant Q183AEscherichia coli(6S)-5-methyltetrahydrofolate0.03——pH 7.2, 4 °CPMID 29407039Open
methylenetetrahydrofolate reductase (MTHFR)variant Q183AEscherichia colimenadione—1.5 × 10⁻⁴—pH 7.2, 4 °CPMID 29407039Open
methylenetetrahydrofolate reductase (MTHFR)variant Q183AEscherichia coliNADHfit to Eq 7 (ping-pong double-substrate inhibition)15.30.01511.01 × 10³ calc.pH 7.2, 4 °CPMID 29407039Open
methylenetetrahydrofolate reductase (MTHFR)variant Q183AEscherichia coliNADHcosubstrate CH2-H4folate (300 µM)0.25——pH 7.2, 4 °CPMID 29407039Open
methylenetetrahydrofolate reductase (MTHFR)variant Q183EEscherichia coli(6R)-5,10-methylenetetrahydrofolate2.70.10825 calc.pH 7.2, 4 °CPMID 29407039Open
methylenetetrahydrofolate reductase (MTHFR)variant Q183EEscherichia coli(6S)-5-methyltetrahydrofolate0.150.1151.3 calc.pH 7.2, 4 °CPMID 29407039Open
methylenetetrahydrofolate reductase (MTHFR)variant Q183EEscherichia colimenadione15.70.00374.2 × 10³ calc.pH 7.2, 4 °CPMID 29407039Open
methylenetetrahydrofolate reductase (MTHFR)variant Q183EEscherichia coliNADHfit to Eq 6 (ping-pong single-substrate inhibition by NADH)140.0204690 calc.pH 7.2, 4 °CPMID 29407039Open
methylenetetrahydrofolate reductase (MTHFR)variant Q183EEscherichia coliNADHcosubstrate CH2-H4folate (500 µM)2.60.0066390 calc.pH 7.2, 4 °CPMID 29407039Open