Values CatLog read from this paper
A role for glutamine 183 in the folate oxidative half-reaction of methylenetetrahydrofolate reductase from Escherichia coli
PMID 29407039doi:10.1016/j.abb.2018.01.014Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (22 values) from this paper: methylenetetrahydrofolate reductase (MTHFR) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 9 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| methylenetetrahydrofolate reductase (MTHFR) | Escherichia coli | (6S)-5-methyltetrahydrofolate | 0.47 | 0.03 | 16 calc. | pH 7.2, 4 °C | Open record: methylenetetrahydrofolate reductase (MTHFR), (6S)-5-methyltetrahydrofolate |
| methylenetetrahydrofolate reductase (MTHFR) | Escherichia coli | menadione | — | 2.1 × 10⁻⁴ | — | pH 7.2, 4 °C | Open record: methylenetetrahydrofolate reductase (MTHFR), menadione |
| methylenetetrahydrofolate reductase (MTHFR) | Escherichia coli | NADH | 18.2 | 0.025 | 730 calc. | pH 7.2, 4 °C | Open record: methylenetetrahydrofolate reductase (MTHFR), NADH |
| methylenetetrahydrofolate reductase (MTHFR)variant Q183A | Escherichia coli | (6R)-5,10-methylenetetrahydrofolate | 0.29 | 0.104 | 2.8 calc. | pH 7.2, 4 °C | Open record: methylenetetrahydrofolate reductase (MTHFR) variant Q183A, (6R)-5,10-methylenetetrahydrofolate |
| methylenetetrahydrofolate reductase (MTHFR)variant Q183A | Escherichia coli | (6S)-5-methyltetrahydrofolate | 0.03 | — | — | pH 7.2, 4 °C | Open record: methylenetetrahydrofolate reductase (MTHFR) variant Q183A, (6S)-5-methyltetrahydrofolate |
| methylenetetrahydrofolate reductase (MTHFR)variant Q183A | Escherichia coli | menadione | — | 1.5 × 10⁻⁴ | — | pH 7.2, 4 °C | Open record: methylenetetrahydrofolate reductase (MTHFR) variant Q183A, menadione |
| methylenetetrahydrofolate reductase (MTHFR)variant Q183A | Escherichia coli | NADHfit to Eq 7 (ping-pong double-substrate inhibition) | 15.3 | 0.0151 | 1.01 × 10³ calc. | pH 7.2, 4 °C | Open record: methylenetetrahydrofolate reductase (MTHFR) variant Q183A, NADH |
| methylenetetrahydrofolate reductase (MTHFR)variant Q183A | Escherichia coli | NADHcosubstrate CH2-H4folate (300 µM) | 0.25 | — | — | pH 7.2, 4 °C | Open record: methylenetetrahydrofolate reductase (MTHFR) variant Q183A, NADH |
| methylenetetrahydrofolate reductase (MTHFR)variant Q183E | Escherichia coli | (6R)-5,10-methylenetetrahydrofolate | 2.7 | 0.108 | 25 calc. | pH 7.2, 4 °C | Open record: methylenetetrahydrofolate reductase (MTHFR) variant Q183E, (6R)-5,10-methylenetetrahydrofolate |
| methylenetetrahydrofolate reductase (MTHFR)variant Q183E | Escherichia coli | (6S)-5-methyltetrahydrofolate | 0.15 | 0.115 | 1.3 calc. | pH 7.2, 4 °C | Open record: methylenetetrahydrofolate reductase (MTHFR) variant Q183E, (6S)-5-methyltetrahydrofolate |
| methylenetetrahydrofolate reductase (MTHFR)variant Q183E | Escherichia coli | menadione | 15.7 | 0.0037 | 4.2 × 10³ calc. | pH 7.2, 4 °C | Open record: methylenetetrahydrofolate reductase (MTHFR) variant Q183E, menadione |
| methylenetetrahydrofolate reductase (MTHFR)variant Q183E | Escherichia coli | NADHfit to Eq 6 (ping-pong single-substrate inhibition by NADH) | 14 | 0.0204 | 690 calc. | pH 7.2, 4 °C | Open record: methylenetetrahydrofolate reductase (MTHFR) variant Q183E, NADH |
| methylenetetrahydrofolate reductase (MTHFR)variant Q183E | Escherichia coli | NADHcosubstrate CH2-H4folate (500 µM) | 2.6 | 0.0066 | 390 calc. | pH 7.2, 4 °C | Open record: methylenetetrahydrofolate reductase (MTHFR) variant Q183E, NADH |