Values CatLog read from this paper
Functional role for the conformationally mobile phenylalanine 223 in the reaction of methylenetetrahydrofolate reductase from Escherichia coli
PMID 19610625doi:10.1021/bi9007325Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (10 values) from this paper: methylenetetrahydrofolate reductase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| methylenetetrahydrofolate reductasevariant F223A | Escherichia coli | (6R)-5,10-methylenetetrahydrofolate | 2.9 | 0.093 | 31 calc. | pH 7.2, 25 °C | Open record: methylenetetrahydrofolate reductase variant F223A, (6R)-5,10-methylenetetrahydrofolate |
| methylenetetrahydrofolate reductasevariant F223A | Escherichia coli | NADHNADH-menadione oxidoreductase assay (aerobic) | 22 | 0.585 | 38 calc. | pH 7.2, 25 °C | Open record: methylenetetrahydrofolate reductase variant F223A, NADH |
| methylenetetrahydrofolate reductasevariant F223A | Escherichia coli | NADHNADH-CH2-H4folate oxidoreductase assay (anaerobic) | — | 0.14 | — | pH 7.2, 25 °C | Open record: methylenetetrahydrofolate reductase variant F223A, NADH |
| methylenetetrahydrofolate reductasevariant F223L | Escherichia coli | (6R)-5,10-methylenetetrahydrofolate | 14 | 0.008 | 1.8 × 10³ calc. | pH 7.2, 25 °C | Open record: methylenetetrahydrofolate reductase variant F223L, (6R)-5,10-methylenetetrahydrofolate |
| methylenetetrahydrofolate reductasevariant F223L | Escherichia coli | NADHNADH-menadione oxidoreductase assay (aerobic) | 31 | 0.47 | 66 calc. | pH 7.2, 25 °C | Open record: methylenetetrahydrofolate reductase variant F223L, NADH |
| methylenetetrahydrofolate reductasevariant F223L | Escherichia coli | NADHNADH-CH2-H4folate oxidoreductase assay (anaerobic) | — | 0.236 | — | pH 7.2, 25 °C | Open record: methylenetetrahydrofolate reductase variant F223L, NADH |