Values CatLog read from this paper

Probing catalysis by Escherichia coli dTDP-glucose-4,6-dehydratase: identification and preliminary characterization of functional amino acid residues at the active site

PMID 11380254doi:10.1021/bi010441aOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 21 records (61 values) from this paper: dTDP-glucose-4,6-dehydratase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.2.1.46

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
dTDP-glucose-4,6-dehydratase	Escherichia coli	thymidine-5'-diphospho-[1,2,3,4,5,6,6-2H7]-alpha-D-glucose	0.46	—	420	pH 7.5, 18 °C	Open record: dTDP-glucose-4,6-dehydratase, thymidine-5'-diphospho-[1,2,3,4,5,6,6-2H7]-alpha-D-glucose
dTDP-glucose-4,6-dehydratase	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	4.9	0.006	820	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratase	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	1.9	—	850	pH 7.5, 18 °C	Open record: dTDP-glucose-4,6-dehydratase, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratase	Escherichia coli	UDP-glucose	0.024	30	7.9 × 10⁻⁴	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase, UDP-glucose
dTDP-glucose-4,6-dehydratasevariant C187A	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	0.52	0.001	500	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase variant C187A, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratasevariant C187S	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	1.2	0.0015	800	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase variant C187S, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratasevariant D135A	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	0.022	0.007	3	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase variant D135A, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratasevariant D135N	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	0.0395	0.0079	5	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase variant D135N, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratasevariant D135N/E136Q	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	0.024	0.019	1.3	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase variant D135N/E136Q, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratasevariant E136A	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	0.017	0.012	1.4	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase variant E136A, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratasevariant E136Q	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	0.073	0.009	7	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase variant E136Q, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratasevariant E198Q	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	0.019	0.011	1.6	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase variant E198Q, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratasevariant H232A	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	0.085	0.0033	26	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase variant H232A, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratasevariant H232N	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	0.72	0.005	150	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase variant H232N, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratasevariant H232Q	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	0.043	0.008	6	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase variant H232Q, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratasevariant K199M	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	0.0425	0.008	5.3	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase variant K199M, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratasevariant K199R	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	0.017	0.013	1.3	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase variant K199R, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratasevariant N190A	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	0.0089	0.0055	1.6	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase variant N190A, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratasevariant N190D	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	0.0111	0.025	0.44	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase variant N190D, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratasevariant N190H	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	0.0226	0.0073	3.1	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase variant N190H, thymidine-5'-diphospho-alpha-D-glucose
dTDP-glucose-4,6-dehydratasevariant Y301F	Escherichia coli	thymidine-5'-diphospho-alpha-D-glucose	0.042	0.031	1.4	pH 7.5, 37 °C	Open record: dTDP-glucose-4,6-dehydratase variant Y301F, thymidine-5'-diphospho-alpha-D-glucose