EC 4.2.1.46: dTDP-glucose 4,6-dehydratase
LyasesOpen in Browse
CatLog read 42 records for EC 4.2.1.46 from 4 papers, for 2 organisms. Names in these papers: dTDP-glucose-4,6-dehydratase, dTDP-glucose 4,6-dehydratase, dTDP-D-glucose 4,6-dehydratase (RmlB).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 15 records for EC 4.2.1.46 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.2.1.46 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Probing catalysis by Escherichia coli dTDP-glucose-4,6-dehydratase: identification and preliminary characterization of functional amino acid residues at the active site | Escherichia coli | 21 |
| Mechanistic roles of Thr134, Tyr160, and Lys 164 in the reaction catalyzed by dTDP-glucose 4,6-dehydratase | Escherichia coli | 10 |
| Dehydration is catalyzed by glutamate-136 and aspartic acid-135 active site residues in Escherichia coli dTDP-glucose 4,6-dehydratase | Escherichia coli | 10 |
| Homologs of the Rml enzymes from Salmonella enterica are responsible for dTDP-beta-L-rhamnose biosynthesis in the gram-positive thermophile Aneurinibacillus thermoaerophilus DSM 10155 | Aneurinibacillus thermoaerophilus | 1 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| dTDP-D-glucose 4,6-dehydratase (RmlB) | Aneurinibacillus thermoaerophilus | dTDP-alpha-D-glucose | 1.9 | 0.012 | 160 calc. | 25 °C | PMID 12147463 | Open record: dTDP-D-glucose 4,6-dehydratase (RmlB), dTDP-alpha-D-glucose |
| dTDP-glucose 4,6-dehydratase | Escherichia coli | dTDP-6-deoxy-6-fluoroglucose | 0.044 | 0.274 | 0.16 | pH 7.5, 37 °C | PMID 11601973 | Open record: dTDP-glucose 4,6-dehydratase, dTDP-6-deoxy-6-fluoroglucose |
| dTDP-glucose 4,6-dehydratasevariant D135A | Escherichia coli | dTDP-6-deoxy-6-fluoroglucose | 0.029 | — | — | pH 7.5, 37 °C | PMID 11601973 | Open record: dTDP-glucose 4,6-dehydratase variant D135A, dTDP-6-deoxy-6-fluoroglucose |
| dTDP-glucose 4,6-dehydratasevariant D135N | Escherichia coli | dTDP-6-deoxy-6-fluoroglucose | 0.053 | — | — | pH 7.5, 37 °C | PMID 11601973 | Open record: dTDP-glucose 4,6-dehydratase variant D135N, dTDP-6-deoxy-6-fluoroglucose |
| dTDP-glucose 4,6-dehydratasevariant D135N/E136Q | Escherichia coli | dTDP-6-deoxy-6-fluoroglucose | 1.3 × 10⁻⁴ | — | — | pH 7.5, 37 °C | PMID 11601973 | Open record: dTDP-glucose 4,6-dehydratase variant D135N/E136Q, dTDP-6-deoxy-6-fluoroglucose |
| dTDP-glucose 4,6-dehydratasevariant E136A | Escherichia coli | dTDP-6-deoxy-6-fluoroglucose | 6.4 × 10⁻⁵ | — | — | pH 7.5, 37 °C | PMID 11601973 | Open record: dTDP-glucose 4,6-dehydratase variant E136A, dTDP-6-deoxy-6-fluoroglucose |
| dTDP-glucose 4,6-dehydratasevariant E136Q | Escherichia coli | dTDP-6-deoxy-6-fluoroglucose | 6.4 × 10⁻⁴ | — | — | pH 7.5, 37 °C | PMID 11601973 | Open record: dTDP-glucose 4,6-dehydratase variant E136Q, dTDP-6-deoxy-6-fluoroglucose |
| dTDP-glucose 4,6-dehydratasevariant E198Q | Escherichia coli | dTDP-6-deoxy-6-fluoroglucose | 2.3 × 10⁻⁴ | — | — | pH 7.5, 37 °C | PMID 11601973 | Open record: dTDP-glucose 4,6-dehydratase variant E198Q, dTDP-6-deoxy-6-fluoroglucose |
| dTDP-glucose 4,6-dehydratasevariant K164A | Escherichia coli | thymidine 5'-diphospho-alpha-D-glucose | 0.14 | 0.09 | 1.58 | pH 7.5, 37 °C | PMID 11478886 | Open record: dTDP-glucose 4,6-dehydratase variant K164A, thymidine 5'-diphospho-alpha-D-glucose |
| dTDP-glucose 4,6-dehydratasevariant K164M | Escherichia coli | thymidine 5'-diphospho-alpha-D-glucose | 0.051 | 0.052 | 1 | pH 7.5, 37 °C | PMID 11478886 | Open record: dTDP-glucose 4,6-dehydratase variant K164M, thymidine 5'-diphospho-alpha-D-glucose |
| dTDP-glucose 4,6-dehydratasevariant K164M | Escherichia coli | thymidine 5'-diphospho-alpha-D-glucose | 0.08 | — | — | 18 °C | PMID 11478886 | Open record: dTDP-glucose 4,6-dehydratase variant K164M, thymidine 5'-diphospho-alpha-D-glucose |
| dTDP-glucose 4,6-dehydratasevariant K199M | Escherichia coli | dTDP-6-deoxy-6-fluoroglucose | 1.7 × 10⁻⁴ | — | — | pH 7.5, 37 °C | PMID 11601973 | Open record: dTDP-glucose 4,6-dehydratase variant K199M, dTDP-6-deoxy-6-fluoroglucose |
| dTDP-glucose 4,6-dehydratasevariant K199R | Escherichia coli | dTDP-6-deoxy-6-fluoroglucose | 6.5 × 10⁻⁵ | — | — | pH 7.5, 37 °C | PMID 11601973 | Open record: dTDP-glucose 4,6-dehydratase variant K199R, dTDP-6-deoxy-6-fluoroglucose |
| dTDP-glucose 4,6-dehydratasevariant T134A | Escherichia coli | thymidine 5'-diphospho-alpha-D-glucose | 0.021 | 0.007 | 2.9 | pH 7.5, 37 °C | PMID 11478886 | Open record: dTDP-glucose 4,6-dehydratase variant T134A, thymidine 5'-diphospho-alpha-D-glucose |
| dTDP-glucose 4,6-dehydratasevariant T134A | Escherichia coli | thymidine 5'-diphospho-alpha-D-glucose | 0.015 | — | — | 18 °C | PMID 11478886 | Open record: dTDP-glucose 4,6-dehydratase variant T134A, thymidine 5'-diphospho-alpha-D-glucose |
| dTDP-glucose 4,6-dehydratasevariant T134S | Escherichia coli | thymidine 5'-diphospho-alpha-D-glucose | 2.4 | 0.022 | 109 | pH 7.5, 37 °C | PMID 11478886 | Open record: dTDP-glucose 4,6-dehydratase variant T134S, thymidine 5'-diphospho-alpha-D-glucose |
| dTDP-glucose 4,6-dehydratasevariant T134V | Escherichia coli | thymidine 5'-diphospho-alpha-D-glucose | 0.0207 | 0.02 | 1.04 | pH 7.5, 37 °C | PMID 11478886 | Open record: dTDP-glucose 4,6-dehydratase variant T134V, thymidine 5'-diphospho-alpha-D-glucose |
| dTDP-glucose 4,6-dehydratasevariant Y160A | Escherichia coli | thymidine 5'-diphospho-alpha-D-glucose | 0.0198 | 0.017 | 1.2 | pH 7.5, 37 °C | PMID 11478886 | Open record: dTDP-glucose 4,6-dehydratase variant Y160A, thymidine 5'-diphospho-alpha-D-glucose |
| dTDP-glucose 4,6-dehydratasevariant Y160F | Escherichia coli | thymidine 5'-diphospho-alpha-D-glucose | 0.0258 | 0.0073 | 3.5 | pH 7.5, 37 °C | PMID 11478886 | Open record: dTDP-glucose 4,6-dehydratase variant Y160F, thymidine 5'-diphospho-alpha-D-glucose |
| dTDP-glucose 4,6-dehydratasevariant Y160F | Escherichia coli | thymidine 5'-diphospho-alpha-D-glucose | 0.011 | — | — | 18 °C | PMID 11478886 | Open record: dTDP-glucose 4,6-dehydratase variant Y160F, thymidine 5'-diphospho-alpha-D-glucose |
| dTDP-glucose 4,6-dehydratasevariant Y301F | Escherichia coli | dTDP-6-deoxy-6-fluoroglucose | 0.0014 | — | — | pH 7.5, 37 °C | PMID 11601973 | Open record: dTDP-glucose 4,6-dehydratase variant Y301F, dTDP-6-deoxy-6-fluoroglucose |
| dTDP-glucose-4,6-dehydratase | Escherichia coli | thymidine-5'-diphospho-[1,2,3,4,5,6,6-2H7]-alpha-D-glucose | 0.46 | — | 420 | pH 7.5, 18 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase, thymidine-5'-diphospho-[1,2,3,4,5,6,6-2H7]-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratase | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 4.9 | 0.006 | 820 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratase | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 1.9 | — | 850 | pH 7.5, 18 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratase | Escherichia coli | UDP-glucose | 0.024 | 30 | 7.9 × 10⁻⁴ | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase, UDP-glucose |
| dTDP-glucose-4,6-dehydratasevariant C187A | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 0.52 | 0.001 | 500 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase variant C187A, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratasevariant C187S | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 1.2 | 0.0015 | 800 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase variant C187S, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratasevariant D135A | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 0.022 | 0.007 | 3 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase variant D135A, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratasevariant D135N | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 0.0395 | 0.0079 | 5 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase variant D135N, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratasevariant D135N/E136Q | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 0.024 | 0.019 | 1.3 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase variant D135N/E136Q, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratasevariant E136A | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 0.017 | 0.012 | 1.4 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase variant E136A, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratasevariant E136Q | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 0.073 | 0.009 | 7 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase variant E136Q, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratasevariant E198Q | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 0.019 | 0.011 | 1.6 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase variant E198Q, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratasevariant H232A | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 0.085 | 0.0033 | 26 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase variant H232A, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratasevariant H232N | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 0.72 | 0.005 | 150 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase variant H232N, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratasevariant H232Q | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 0.043 | 0.008 | 6 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase variant H232Q, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratasevariant K199M | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 0.0425 | 0.008 | 5.3 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase variant K199M, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratasevariant K199R | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 0.017 | 0.013 | 1.3 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase variant K199R, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratasevariant N190A | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 0.0089 | 0.0055 | 1.6 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase variant N190A, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratasevariant N190D | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 0.0111 | 0.025 | 0.44 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase variant N190D, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratasevariant N190H | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 0.0226 | 0.0073 | 3.1 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase variant N190H, thymidine-5'-diphospho-alpha-D-glucose |
| dTDP-glucose-4,6-dehydratasevariant Y301F | Escherichia coli | thymidine-5'-diphospho-alpha-D-glucose | 0.042 | 0.031 | 1.4 | pH 7.5, 37 °C | PMID 11380254 | Open record: dTDP-glucose-4,6-dehydratase variant Y301F, thymidine-5'-diphospho-alpha-D-glucose |