CatLogenzyme kinetics catalog

Values CatLog read from this paper

Dehydration is catalyzed by glutamate-136 and aspartic acid-135 active site residues in Escherichia coli dTDP-glucose 4,6-dehydratase

PMID 11601973doi:10.1021/bi011138cOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 10 records (12 values) from this paper: dTDP-glucose 4,6-dehydratase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.2.1.46

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
dTDP-glucose 4,6-dehydrataseEscherichia colidTDP-6-deoxy-6-fluoroglucose0.0440.2740.16pH 7.5, 37 °COpen
dTDP-glucose 4,6-dehydratasevariant D135AEscherichia colidTDP-6-deoxy-6-fluoroglucose0.029——pH 7.5, 37 °COpen
dTDP-glucose 4,6-dehydratasevariant D135NEscherichia colidTDP-6-deoxy-6-fluoroglucose0.053——pH 7.5, 37 °COpen
dTDP-glucose 4,6-dehydratasevariant D135N/E136QEscherichia colidTDP-6-deoxy-6-fluoroglucose1.3 × 10⁻⁴——pH 7.5, 37 °COpen
dTDP-glucose 4,6-dehydratasevariant E136AEscherichia colidTDP-6-deoxy-6-fluoroglucose6.4 × 10⁻⁵——pH 7.5, 37 °COpen
dTDP-glucose 4,6-dehydratasevariant E136QEscherichia colidTDP-6-deoxy-6-fluoroglucose6.4 × 10⁻⁴——pH 7.5, 37 °COpen
dTDP-glucose 4,6-dehydratasevariant E198QEscherichia colidTDP-6-deoxy-6-fluoroglucose2.3 × 10⁻⁴——pH 7.5, 37 °COpen
dTDP-glucose 4,6-dehydratasevariant K199MEscherichia colidTDP-6-deoxy-6-fluoroglucose1.7 × 10⁻⁴——pH 7.5, 37 °COpen
dTDP-glucose 4,6-dehydratasevariant K199REscherichia colidTDP-6-deoxy-6-fluoroglucose6.5 × 10⁻⁵——pH 7.5, 37 °COpen
dTDP-glucose 4,6-dehydratasevariant Y301FEscherichia colidTDP-6-deoxy-6-fluoroglucose0.0014——pH 7.5, 37 °COpen