CatLogenzyme kinetics catalog

Values CatLog read from this paper

Human endonuclease III acts preferentially on DNA damage opposite guanine residues in DNA

PMID 11380260doi:10.1021/bi0028901Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 12 records (24 values) from this paper: human endonuclease III homologue 1 (hNTH1) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 12 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 4.2.99.18

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
human endonuclease III homologue 1 (hNTH1)Homo sapiens32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex)2 mM MgCl2 (magnesium)3.3 × 10⁻⁴2 × 10⁻⁷1.7 × 10³ calc.pH 7.5, 37 °COpen
human endonuclease III homologue 1 (hNTH1)Homo sapiens32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex)EDTA instead of magnesium (EDTA concentration not stated)5 × 10⁻⁴7 × 10⁻⁸7.1 × 10³ calc.pH 7.5, 37 °COpen
human endonuclease III homologue 1 (hNTH1)Homo sapiens32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex)2 mM MgCl2 (magnesium)8.3 × 10⁻⁴5 × 10⁻⁸1.7 × 10⁴ calc.pH 7.5, 37 °COpen
human endonuclease III homologue 1 (hNTH1)Homo sapiens32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex)EDTA instead of magnesium (EDTA concentration not stated)6.7 × 10⁻⁴5 × 10⁻⁸1.3 × 10⁴ calc.pH 7.5, 37 °COpen
human endonuclease III homologue 1 (hNTH1)variant +G212Homo sapiens32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex)2 mM MgCl2 (magnesium)3.5 × 10⁻⁵1.7 × 10⁻⁶21 calc.pH 7.5, 37 °COpen
human endonuclease III homologue 1 (hNTH1)variant +G212Homo sapiens32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex)EDTA instead of magnesium (EDTA concentration not stated)6.7 × 10⁻⁵3.5 × 10⁻⁶19 calc.pH 7.5, 37 °COpen
human endonuclease III homologue 1 (hNTH1)variant +G212Homo sapiens32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex)2 mM MgCl2 (magnesium)2.3 × 10⁻⁴1.2 × 10⁻⁶190 calc.pH 7.5, 37 °COpen
human endonuclease III homologue 1 (hNTH1)variant +G212Homo sapiens32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex)EDTA instead of magnesium (EDTA concentration not stated)1.17 × 10⁻⁴1 × 10⁻⁶120 calc.pH 7.5, 37 °COpen
human endonuclease III homologue 1 (hNTH1)variant P211RHomo sapiens32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex)2 mM MgCl2 (magnesium)1 × 10⁻⁴1.4 × 10⁻⁷710 calc.pH 7.5, 37 °COpen
human endonuclease III homologue 1 (hNTH1)variant P211RHomo sapiens32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex)EDTA instead of magnesium (EDTA concentration not stated)5 × 10⁻⁴2 × 10⁻⁷2.5 × 10³ calc.pH 7.5, 37 °COpen
human endonuclease III homologue 1 (hNTH1)variant P211RHomo sapiens32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex)2 mM MgCl2 (magnesium)5 × 10⁻⁴4.8 × 10⁻⁸1.0 × 10⁴ calc.pH 7.5, 37 °COpen
human endonuclease III homologue 1 (hNTH1)variant P211RHomo sapiens32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex)EDTA instead of magnesium (EDTA concentration not stated)5.8 × 10⁻⁴9 × 10⁻⁸6.5 × 10³ calc.pH 7.5, 37 °COpen