EC 4.2.99.18: DNA-(apurinic or apyrimidinic site) lyase
LyasesOpen in Browse
CatLog read 156 records for EC 4.2.99.18 from 6 papers, for 2 organisms. Names in these papers: endonuclease VIII (Nei), hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), formamidopyrimidine-DNA glycosylase (Fpg, MutM), human endonuclease III homologue 1 (hNTH1) and 2 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 96 records for EC 4.2.99.18 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.2.99.18 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Catalytic mechanism of Escherichia coli endonuclease VIII: roles of the intercalation loop and the zinc finger | Escherichia coli | 75 |
| Excision of products of oxidative DNA base damage by human NTH1 protein | Homo sapiens | 39 |
| Substrate discrimination by formamidopyrimidine-DNA glycosylase: a mutational analysis | Escherichia coli | 18 |
| Human endonuclease III acts preferentially on DNA damage opposite guanine residues in DNA | Homo sapiens | 12 |
| A Comparative Study for the Incorporation of 8-oxo-dATP in DNA by Human DNA Polymerases | Homo sapiens | 8 |
| A monofunctional-like mutant of DNA glycosylase NTHL1 changes the dynamics of DNA repair during acute oxidative stress | Homo sapiens | 4 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| DNA polymerase β (Pol β) | Homo sapiens | 2'-deoxy-7,8-dihydro-8-oxoadenosine-5'-triphosphateincorporation opposite template T at the +1 position | 0.0055 | 0.0545 | 0.105 | pH 7.4, 37 °C | PMID 41898401 | Open record: DNA polymerase β (Pol β), 2'-deoxy-7,8-dihydro-8-oxoadenosine-5'-triphosphate |
| DNA polymerase β (Pol β) | Homo sapiens | 2'-deoxy-7,8-dihydro-8-oxoadenosine-5'-triphosphateincorporation opposite template T at the +1 position | 0.0015 | 0.0221 | 0.085 | pH 7.4, 37 °C | PMID 41898401 | Open record: DNA polymerase β (Pol β), 2'-deoxy-7,8-dihydro-8-oxoadenosine-5'-triphosphate |
| DNA polymerase β (Pol β) | Homo sapiens | 2'-deoxy-7,8-dihydro-8-oxoadenosine-5'-triphosphateincorporation opposite template G at the +1 position | 2 × 10⁻⁴ | 0.1442 | 0.0015 | pH 7.4, 37 °C | PMID 41898401 | Open record: DNA polymerase β (Pol β), 2'-deoxy-7,8-dihydro-8-oxoadenosine-5'-triphosphate |
| DNA polymerase β (Pol β) | Homo sapiens | 2'-deoxy-7,8-dihydro-8-oxoadenosine-5'-triphosphateincorporation opposite template G at the +1 position | 1.5 × 10⁻⁵ | 0.0893 | 2.3 × 10⁻⁴ | pH 7.4, 37 °C | PMID 41898401 | Open record: DNA polymerase β (Pol β), 2'-deoxy-7,8-dihydro-8-oxoadenosine-5'-triphosphate |
| DNA polymerase β (Pol β) | Homo sapiens | 2'-deoxyadenosine 5'-triphosphateincorporation opposite template T at the +1 position | 0.91 | 0.0152 | 63 | pH 7.4, 37 °C | PMID 41898401 | Open record: DNA polymerase β (Pol β), 2'-deoxyadenosine 5'-triphosphate |
| DNA polymerase β (Pol β) | Homo sapiens | 2'-deoxyadenosine 5'-triphosphateincorporation opposite template T at the +1 position | 0.325 | 0.0953 | 4 | pH 7.4, 37 °C | PMID 41898401 | Open record: DNA polymerase β (Pol β), 2'-deoxyadenosine 5'-triphosphate |
| DNA polymerase β (Pol β) | Homo sapiens | 2'-deoxyadenosine 5'-triphosphateincorporation opposite template G at the +1 position | 2.2 × 10⁻⁴ | 0.1816 | 0.0013 | pH 7.4, 37 °C | PMID 41898401 | Open record: DNA polymerase β (Pol β), 2'-deoxyadenosine 5'-triphosphate |
| DNA polymerase β (Pol β) | Homo sapiens | 2'-deoxyadenosine 5'-triphosphateincorporation opposite template G at the +1 position | 4 × 10⁻⁵ | 0.1403 | 4.3 × 10⁻⁴ | pH 7.4, 37 °C | PMID 41898401 | Open record: DNA polymerase β (Pol β), 2'-deoxyadenosine 5'-triphosphate |
| endonuclease III-like protein 1 (NTHL1), bifunctional DNA glycosylase | Homo sapiens | 22-mer oligonucleotide DNA duplex containing thymine glycol (Tg) opposite A (fluorophore/quencher molecular beacon)NTHL1 alone (no APE1) | — | 9.7 × 10⁻⁷ | — | pH 8, 25 °C | PMID 41763309 | Open record: endonuclease III-like protein 1 (NTHL1), bifunctional DNA glycosylase, 22-mer oligonucleotide DNA duplex containing thymine glycol (Tg) opposite A (fluorophore/quencher molecular beacon) |
| endonuclease III-like protein 1 (NTHL1), bifunctional DNA glycosylase | Homo sapiens | 22-mer oligonucleotide DNA duplex containing thymine glycol (Tg) opposite A (fluorophore/quencher molecular beacon)with 2 nM APE1 (in excess); 5 nM NTHL1 | — | 1.02 × 10⁻⁵ | — | pH 8, 25 °C | PMID 41763309 | Open record: endonuclease III-like protein 1 (NTHL1), bifunctional DNA glycosylase, 22-mer oligonucleotide DNA duplex containing thymine glycol (Tg) opposite A (fluorophore/quencher molecular beacon) |
| endonuclease III-like protein 1 (NTHL1), bifunctional DNA glycosylasevariant L224K | Homo sapiens | 22-mer oligonucleotide DNA duplex containing thymine glycol (Tg) opposite A (fluorophore/quencher molecular beacon)NTHL1 alone (no APE1) | — | 2.54 × 10⁻⁵ | — | pH 8, 25 °C | PMID 41763309 | Open record: endonuclease III-like protein 1 (NTHL1), bifunctional DNA glycosylase variant L224K, 22-mer oligonucleotide DNA duplex containing thymine glycol (Tg) opposite A (fluorophore/quencher molecular beacon) |
| endonuclease III-like protein 1 (NTHL1), bifunctional DNA glycosylasevariant L224K | Homo sapiens | 22-mer oligonucleotide DNA duplex containing thymine glycol (Tg) opposite A (fluorophore/quencher molecular beacon)with 2 nM APE1 (in excess); 5 nM NTHL1 | — | 1.02 × 10⁻⁴ | — | pH 8, 25 °C | PMID 41763309 | Open record: endonuclease III-like protein 1 (NTHL1), bifunctional DNA glycosylase variant L224K, 22-mer oligonucleotide DNA duplex containing thymine glycol (Tg) opposite A (fluorophore/quencher molecular beacon) |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), adenine (A) opposite the lesion | 5.8 × 10⁻⁴ | 1.6 × 10⁻⁴ | 3.7 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), adenine (A) opposite the lesion |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), cytosine (C) opposite the lesion | 0.0082 | 6.6 × 10⁻⁶ | 1.23 × 10³ | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), cytosine (C) opposite the lesion |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), guanine (G) opposite the lesion | 0.072 | 8.5 × 10⁻⁶ | 8.5 × 10³ | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), guanine (G) opposite the lesion |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), thymine (T) opposite the lesion | 0.0032 | 3.5 × 10⁻⁶ | 900 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), thymine (T) opposite the lesion |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), adenine (A) opposite the lesion | 0.0078 | 6.1 × 10⁻⁴ | 13 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), adenine (A) opposite the lesion |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), cytosine (C) opposite the lesion | 0.0158 | 6.8 × 10⁻⁴ | 23 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), cytosine (C) opposite the lesion |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), guanine (G) opposite the lesion | 0.0132 | 5.8 × 10⁻⁴ | 23 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), guanine (G) opposite the lesion |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), thymine (T) opposite the lesion | 0.023 | 0.0013 | 18 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), thymine (T) opposite the lesion |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, adenine (A) opposite the lesion | 0.0107 | 8.3 × 10⁻⁸ | 1.28 × 10⁵ | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, adenine (A) opposite the lesion |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, guanine (G) opposite the lesion | 0.0088 | 1.1 × 10⁻⁷ | 8 × 10⁴ | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, guanine (G) opposite the lesion |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), adenine (A) opposite the lesion | 0.035 | 3.2 × 10⁻⁶ | 1.1 × 10⁴ | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), adenine (A) opposite the lesion |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), cytosine (C) opposite the lesion | 0.042 | 4.4 × 10⁻⁶ | 9.5 × 10³ | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), cytosine (C) opposite the lesion |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), guanine (G) opposite the lesion | 0.033 | 2.3 × 10⁻⁶ | 1.45 × 10⁴ | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), guanine (G) opposite the lesion |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), thymine (T) opposite the lesion | 0.048 | 4.4 × 10⁻⁶ | 1.1 × 10⁴ | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), thymine (T) opposite the lesion |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), adenine (A) opposite the lesion | 0.023 | 1.6 × 10⁻⁵ | 1.47 × 10³ | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), adenine (A) opposite the lesion |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), cytosine (C) opposite the lesion | 0.0115 | 4.7 × 10⁻⁶ | 2.5 × 10³ | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), cytosine (C) opposite the lesion |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), guanine (G) opposite the lesion | 0.057 | 2.3 × 10⁻⁶ | 2.5 × 10⁴ | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), guanine (G) opposite the lesion |
| endonuclease VIII (Nei) | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), thymine (T) opposite the lesion | 0.0112 | 4.6 × 10⁻⁶ | 2.5 × 10³ | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei), 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), thymine (T) opposite the lesion |
| endonuclease VIII (Nei)variant Q261A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), adenine (A) opposite the lesion | 2.3 × 10⁻⁴ | 2.6 × 10⁻⁴ | 0.9 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q261A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), adenine (A) opposite the lesion |
| endonuclease VIII (Nei)variant Q261A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), cytosine (C) opposite the lesion | 1.3 × 10⁻⁴ | 2.7 × 10⁻⁴ | 0.5 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q261A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), cytosine (C) opposite the lesion |
| endonuclease VIII (Nei)variant Q261A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), guanine (G) opposite the lesion | 1.5 × 10⁻⁴ | 7.7 × 10⁻⁵ | 2 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q261A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), guanine (G) opposite the lesion |
| endonuclease VIII (Nei)variant Q261A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), thymine (T) opposite the lesion | 6.7 × 10⁻⁵ | 0.0014 | 0.048 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q261A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), thymine (T) opposite the lesion |
| endonuclease VIII (Nei)variant Q261A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), adenine (A) opposite the lesion | — | — | 3.3 × 10⁻⁴ | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q261A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), adenine (A) opposite the lesion |
| endonuclease VIII (Nei)variant Q261A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), cytosine (C) opposite the lesion | — | — | 7.2 × 10⁻⁴ | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q261A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), cytosine (C) opposite the lesion |
| endonuclease VIII (Nei)variant Q261A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), guanine (G) opposite the lesion | — | — | 6.3 × 10⁻⁴ | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q261A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), guanine (G) opposite the lesion |
| endonuclease VIII (Nei)variant Q261A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), thymine (T) opposite the lesion | — | — | 2.3 × 10⁻⁴ | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q261A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), thymine (T) opposite the lesion |
| endonuclease VIII (Nei)variant Q261A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), adenine (A) opposite the lesion | — | — | 3 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q261A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), adenine (A) opposite the lesion |
| endonuclease VIII (Nei)variant Q261A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), cytosine (C) opposite the lesion | — | — | 2.8 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q261A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), cytosine (C) opposite the lesion |
| endonuclease VIII (Nei)variant Q261A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), guanine (G) opposite the lesion | 0.0058 | 2.4 × 10⁻⁴ | 25 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q261A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), guanine (G) opposite the lesion |
| endonuclease VIII (Nei)variant Q261A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), thymine (T) opposite the lesion | — | — | 2.8 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q261A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), thymine (T) opposite the lesion |
| endonuclease VIII (Nei)variant Q261A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), adenine (A) opposite the lesion | — | — | 3 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q261A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), adenine (A) opposite the lesion |
| endonuclease VIII (Nei)variant Q261A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), cytosine (C) opposite the lesion | — | — | 3.2 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q261A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), cytosine (C) opposite the lesion |
| endonuclease VIII (Nei)variant Q261A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), guanine (G) opposite the lesion | 0.0057 | 2.1 × 10⁻⁴ | 27 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q261A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), guanine (G) opposite the lesion |
| endonuclease VIII (Nei)variant Q261A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), thymine (T) opposite the lesion | — | — | 3.3 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q261A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), thymine (T) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), adenine (A) opposite the lesion | 7.2 × 10⁻⁵ | 3.8 × 10⁻⁵ | 1.8 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), adenine (A) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), cytosine (C) opposite the lesion | 0.0133 | 1.4 × 10⁻⁴ | 95 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), cytosine (C) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), guanine (G) opposite the lesion | 6.5 × 10⁻⁵ | 4.2 × 10⁻⁵ | 1.55 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), guanine (G) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), thymine (T) opposite the lesion | 0.0117 | 1.6 × 10⁻⁴ | 73 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), thymine (T) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), adenine (A) opposite the lesion | — | — | 0.077 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), adenine (A) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), cytosine (C) opposite the lesion | — | — | 0.11 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), cytosine (C) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), guanine (G) opposite the lesion | — | — | 0.097 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), guanine (G) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), thymine (T) opposite the lesion | — | — | 0.092 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), thymine (T) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, adenine (A) opposite the lesion | 0.0015 | 2.8 × 10⁻⁵ | 53 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, adenine (A) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, guanine (G) opposite the lesion | 0.0018 | 2.2 × 10⁻⁵ | 83 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, guanine (G) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), adenine (A) opposite the lesion | — | — | 78 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), adenine (A) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), cytosine (C) opposite the lesion | — | — | 92 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), cytosine (C) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), guanine (G) opposite the lesion | 0.016 | 4.9 × 10⁻⁵ | 330 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), guanine (G) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), thymine (T) opposite the lesion | 0.022 | 1.9 × 10⁻⁴ | 113 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), thymine (T) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), adenine (A) opposite the lesion | — | — | 98 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), adenine (A) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), cytosine (C) opposite the lesion | — | — | 93 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), cytosine (C) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), guanine (G) opposite the lesion | — | — | 90 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), guanine (G) opposite the lesion |
| endonuclease VIII (Nei)variant Q69A/L70A/Y71A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), thymine (T) opposite the lesion | — | — | 90 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant Q69A/L70A/Y71A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), thymine (T) opposite the lesion |
| endonuclease VIII (Nei)variant R171A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), adenine (A) opposite the lesion | — | — | 0.062 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant R171A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), adenine (A) opposite the lesion |
| endonuclease VIII (Nei)variant R171A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), cytosine (C) opposite the lesion | — | — | 0.018 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant R171A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), cytosine (C) opposite the lesion |
| endonuclease VIII (Nei)variant R171A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), guanine (G) opposite the lesion | — | — | 0.028 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant R171A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), guanine (G) opposite the lesion |
| endonuclease VIII (Nei)variant R171A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), thymine (T) opposite the lesion | — | — | 0.048 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant R171A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), thymine (T) opposite the lesion |
| endonuclease VIII (Nei)variant R171A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, adenine (A) opposite the lesion | — | — | 0.017 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant R171A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, adenine (A) opposite the lesion |
| endonuclease VIII (Nei)variant R171A | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, guanine (G) opposite the lesion | — | — | 0.01 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant R171A, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, guanine (G) opposite the lesion |
| endonuclease VIII (Nei)variant R171A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), adenine (A) opposite the lesion | — | — | 0.085 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant R171A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), adenine (A) opposite the lesion |
| endonuclease VIII (Nei)variant R171A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), cytosine (C) opposite the lesion | — | — | 0.047 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant R171A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), cytosine (C) opposite the lesion |
| endonuclease VIII (Nei)variant R171A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), guanine (G) opposite the lesion | — | — | 0.073 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant R171A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), guanine (G) opposite the lesion |
| endonuclease VIII (Nei)variant R171A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), thymine (T) opposite the lesion | — | — | 0.088 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant R171A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), thymine (T) opposite the lesion |
| endonuclease VIII (Nei)variant R171A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), adenine (A) opposite the lesion | — | — | 0.043 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant R171A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), adenine (A) opposite the lesion |
| endonuclease VIII (Nei)variant R171A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), cytosine (C) opposite the lesion | — | — | 0.028 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant R171A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), cytosine (C) opposite the lesion |
| endonuclease VIII (Nei)variant R171A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), guanine (G) opposite the lesion | — | — | 0.035 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant R171A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), guanine (G) opposite the lesion |
| endonuclease VIII (Nei)variant R171A | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), thymine (T) opposite the lesion | — | — | 0.05 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant R171A, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), thymine (T) opposite the lesion |
| endonuclease VIII (Nei)variant ΔQLY69-71 | Escherichia coli | 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), guanine (G) opposite the lesion | 4.8 × 10⁻⁵ | 5.4 × 10⁻⁵ | 0.9 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant ΔQLY69-71, 23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), guanine (G) opposite the lesion |
| endonuclease VIII (Nei)variant ΔQLY69-71 | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), adenine (A) opposite the lesion | 0.0025 | 6.6 × 10⁻⁵ | 38 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant ΔQLY69-71, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), adenine (A) opposite the lesion |
| endonuclease VIII (Nei)variant ΔQLY69-71 | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), cytosine (C) opposite the lesion | — | — | 48 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant ΔQLY69-71, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), cytosine (C) opposite the lesion |
| endonuclease VIII (Nei)variant ΔQLY69-71 | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), guanine (G) opposite the lesion | — | — | 52 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant ΔQLY69-71, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), guanine (G) opposite the lesion |
| endonuclease VIII (Nei)variant ΔQLY69-71 | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), thymine (T) opposite the lesion | — | — | 47 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant ΔQLY69-71, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), thymine (T) opposite the lesion |
| endonuclease VIII (Nei)variant ΔQLY69-71 | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), adenine (A) opposite the lesion | — | — | 62 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant ΔQLY69-71, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), adenine (A) opposite the lesion |
| endonuclease VIII (Nei)variant ΔQLY69-71 | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), cytosine (C) opposite the lesion | — | — | 62 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant ΔQLY69-71, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), cytosine (C) opposite the lesion |
| endonuclease VIII (Nei)variant ΔQLY69-71 | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), guanine (G) opposite the lesion | — | — | 62 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant ΔQLY69-71, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), guanine (G) opposite the lesion |
| endonuclease VIII (Nei)variant ΔQLY69-71 | Escherichia coli | 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), thymine (T) opposite the lesion | — | — | 62 | pH 7.5, 25 °C | PMID 17002303 | Open record: endonuclease VIII (Nei) variant ΔQLY69-71, 23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), thymine (T) opposite the lesion |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | 0.0117 | 6.8 × 10⁻⁷ | 1.72 × 10⁴ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite A | 0.0011 | 2.2 × 10⁻⁵ | 50 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite A | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | 0.0053 | 1.05 × 10⁻⁶ | 5.2 × 10³ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite G | 0.0035 | 1 × 10⁻⁷ | 3.43 × 10⁴ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite G | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM) | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite T | 0.0047 | 1.9 × 10⁻⁷ | 2.42 × 10⁴ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM), duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite T | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant H89A | Escherichia coli | duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | 0.0163 | 7 × 10⁻⁷ | 2.33 × 10⁴ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant H89A, duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant H89A | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | 0.0075 | 4.14 × 10⁻⁶ | 1.8 × 10³ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant H89A, duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant H89A/R109A | Escherichia coli | duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | 0.004 | 9.57 × 10⁻⁶ | 420 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant H89A/R109A, duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant H89A/R109A | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | 0.0035 | 4.4 × 10⁻⁴ | 8.3 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant H89A/R109A, duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant K217T | Escherichia coli | duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | 0.0093 | 6.1 × 10⁻⁷ | 1.55 × 10⁴ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant K217T, duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant K217T | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | 0.004 | 4.06 × 10⁻⁶ | 970 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant K217T, duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant R108A | Escherichia coli | duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | 0.0053 | 7 × 10⁻⁷ | 7.7 × 10³ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant R108A, duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant R108A | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite A | 7.5 × 10⁻⁵ | 2.32 × 10⁻⁵ | 3.3 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant R108A, duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite A | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant R108A | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | 4.3 × 10⁻⁴ | 1.49 × 10⁻⁶ | 300 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant R108A, duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant R108A | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite G | 3.8 × 10⁻⁴ | 5.13 × 10⁻⁶ | 83 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant R108A, duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite G | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant R108A | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite T | 3 × 10⁻⁴ | 4.18 × 10⁻⁶ | 67 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant R108A, duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite T | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant R109A | Escherichia coli | duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | 0.0053 | 4.65 × 10⁻⁶ | 1.13 × 10³ | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant R109A, duplex oligodeoxynucleotide containing 5,6-dihydrouracil opposite C | |
| formamidopyrimidine-DNA glycosylase (Fpg, MutM)variant R109A | Escherichia coli | duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | 0.0058 | 2.27 × 10⁻⁴ | 33 | PMID 14607836 | Open record: formamidopyrimidine-DNA glycosylase (Fpg, MutM) variant R109A, duplex oligodeoxynucleotide containing 8-oxo-7,8-dihydroguanine opposite C | |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5,6-dihydroxycytosine (lesion in oxidatively damaged DNA)Table 1 | 2.17 × 10⁻⁴ | — | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5,6-dihydroxycytosine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5,6-dihydroxycytosine (lesion in oxidatively damaged DNA)Table 2 | — | 7.18 × 10⁻⁴ | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5,6-dihydroxycytosine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5,6-dihydroxycytosine (lesion in oxidatively damaged DNA)Table 3 | — | — | 0.302 | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5,6-dihydroxycytosine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxy-6-hydrothymine (lesion in oxidatively damaged DNA)Table 1 | 1.68 × 10⁻⁴ | — | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxy-6-hydrothymine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxy-6-hydrothymine (lesion in oxidatively damaged DNA)Table 2 | — | 3.71 × 10⁻⁴ | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxy-6-hydrothymine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxy-6-hydrothymine (lesion in oxidatively damaged DNA)Table 3 | — | — | 0.455 | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxy-6-hydrothymine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxycytosine (lesion in oxidatively damaged DNA)γ-irradiated under N2O (irradiation/N2O); Table 1 | 3.22 × 10⁻⁴ | — | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxycytosine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxycytosine (lesion in oxidatively damaged DNA)γ-irradiated under air (irradiation/air); Table 1 | 4.67 × 10⁻⁴ | — | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxycytosine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxycytosine (lesion in oxidatively damaged DNA)treated with H2O2/Fe(III)-EDTA (H2O2/Fe-EDTA); Table 1 | 3.48 × 10⁻⁴ | — | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxycytosine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxycytosine (lesion in oxidatively damaged DNA)treated with H2O2/Cu(II) (H2O2/Cu); Table 1 | 4.4 × 10⁻⁴ | — | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxycytosine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxycytosine (lesion in oxidatively damaged DNA)γ-irradiated under N2O (irradiation/N2O); Table 2 | — | 5.48 × 10⁻⁴ | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxycytosine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxycytosine (lesion in oxidatively damaged DNA)γ-irradiated under air (irradiation/air); Table 2 | — | 7.64 × 10⁻⁴ | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxycytosine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxycytosine (lesion in oxidatively damaged DNA)treated with H2O2/Fe(III)-EDTA (H2O2/Fe-EDTA); Table 2 | — | 8.61 × 10⁻⁴ | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxycytosine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxycytosine (lesion in oxidatively damaged DNA)treated with H2O2/Cu(II) (H2O2/Cu); Table 2 | — | 5.76 × 10⁻⁴ | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxycytosine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxycytosine (lesion in oxidatively damaged DNA)γ-irradiated under N2O (irradiation/N2O); Table 3 | — | — | 0.587 | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxycytosine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxycytosine (lesion in oxidatively damaged DNA)γ-irradiated under air (irradiation/air); Table 3 | — | — | 0.612 | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxycytosine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxycytosine (lesion in oxidatively damaged DNA)treated with H2O2/Fe(III)-EDTA (H2O2/Fe-EDTA); Table 3 | — | — | 0.403 | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxycytosine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxycytosine (lesion in oxidatively damaged DNA)treated with H2O2/Cu(II) (H2O2/Cu); Table 3 | — | — | 0.763 | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxycytosine (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxyuracil (lesion in oxidatively damaged DNA)γ-irradiated under air (irradiation/air); Table 1 | 1.67 × 10⁻⁴ | — | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxyuracil (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxyuracil (lesion in oxidatively damaged DNA)treated with H2O2/Fe(III)-EDTA (H2O2/Fe-EDTA); Table 1 | 1 × 10⁻⁴ | — | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxyuracil (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxyuracil (lesion in oxidatively damaged DNA)treated with H2O2/Cu(II) (H2O2/Cu); Table 1 | 3.18 × 10⁻⁴ | — | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxyuracil (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxyuracil (lesion in oxidatively damaged DNA)γ-irradiated under air (irradiation/air); Table 2 | — | 6.35 × 10⁻⁴ | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxyuracil (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxyuracil (lesion in oxidatively damaged DNA)treated with H2O2/Fe(III)-EDTA (H2O2/Fe-EDTA); Table 2 | — | 5.79 × 10⁻⁴ | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxyuracil (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxyuracil (lesion in oxidatively damaged DNA)treated with H2O2/Cu(II) (H2O2/Cu); Table 2 | — | 0.001093 | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxyuracil (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxyuracil (lesion in oxidatively damaged DNA)γ-irradiated under air (irradiation/air); Table 3 | — | — | 0.263 | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxyuracil (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxyuracil (lesion in oxidatively damaged DNA)treated with H2O2/Fe(III)-EDTA (H2O2/Fe-EDTA); Table 3 | — | — | 0.173 | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxyuracil (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | 5-hydroxyuracil (lesion in oxidatively damaged DNA)treated with H2O2/Cu(II) (H2O2/Cu); Table 3 | — | — | 0.29 | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), 5-hydroxyuracil (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | thymine glycol (lesion in oxidatively damaged DNA)γ-irradiated under N2O (irradiation/N2O); Table 1 | 2.88 × 10⁻⁴ | — | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), thymine glycol (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | thymine glycol (lesion in oxidatively damaged DNA)γ-irradiated under air (irradiation/air); Table 1 | 9.4 × 10⁻⁴ | — | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), thymine glycol (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | thymine glycol (lesion in oxidatively damaged DNA)treated with H2O2/Fe(III)-EDTA (H2O2/Fe-EDTA); Table 1 | 7.52 × 10⁻⁴ | — | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), thymine glycol (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | thymine glycol (lesion in oxidatively damaged DNA)treated with H2O2/Cu(II) (H2O2/Cu); Table 1 | 8.25 × 10⁻⁴ | — | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), thymine glycol (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | thymine glycol (lesion in oxidatively damaged DNA)γ-irradiated under N2O (irradiation/N2O); Table 2 | — | 0.001101 | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), thymine glycol (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | thymine glycol (lesion in oxidatively damaged DNA)γ-irradiated under air (irradiation/air); Table 2 | — | 0.003864 | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), thymine glycol (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | thymine glycol (lesion in oxidatively damaged DNA)treated with H2O2/Fe(III)-EDTA (H2O2/Fe-EDTA); Table 2 | — | 0.003438 | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), thymine glycol (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | thymine glycol (lesion in oxidatively damaged DNA)treated with H2O2/Cu(II) (H2O2/Cu); Table 2 | — | 0.00192 | — | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), thymine glycol (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | thymine glycol (lesion in oxidatively damaged DNA)γ-irradiated under N2O (irradiation/N2O); Table 3 | — | — | 0.262 | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), thymine glycol (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | thymine glycol (lesion in oxidatively damaged DNA)γ-irradiated under air (irradiation/air); Table 3 | — | — | 0.243 | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), thymine glycol (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | thymine glycol (lesion in oxidatively damaged DNA)treated with H2O2/Fe(III)-EDTA (H2O2/Fe-EDTA); Table 3 | — | — | 0.218 | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), thymine glycol (lesion in oxidatively damaged DNA) |
| hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III) | Homo sapiens | thymine glycol (lesion in oxidatively damaged DNA)treated with H2O2/Cu(II) (H2O2/Cu); Table 3 | — | — | 0.43 | pH 7.4, 37 °C | PMID 9890904 | Open record: hNTH1 protein (human Nth protein, homologue of E. coli endonuclease III), thymine glycol (lesion in oxidatively damaged DNA) |
| human endonuclease III homologue 1 (hNTH1) | Homo sapiens | 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex)2 mM MgCl2 (magnesium) | 3.3 × 10⁻⁴ | 2 × 10⁻⁷ | 1.7 × 10³ calc. | pH 7.5, 37 °C | PMID 11380260 | Open record: human endonuclease III homologue 1 (hNTH1), 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex) |
| human endonuclease III homologue 1 (hNTH1) | Homo sapiens | 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex)EDTA instead of magnesium (EDTA concentration not stated) | 5 × 10⁻⁴ | 7 × 10⁻⁸ | 7.1 × 10³ calc. | pH 7.5, 37 °C | PMID 11380260 | Open record: human endonuclease III homologue 1 (hNTH1), 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex) |
| human endonuclease III homologue 1 (hNTH1) | Homo sapiens | 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex)2 mM MgCl2 (magnesium) | 8.3 × 10⁻⁴ | 5 × 10⁻⁸ | 1.7 × 10⁴ calc. | pH 7.5, 37 °C | PMID 11380260 | Open record: human endonuclease III homologue 1 (hNTH1), 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex) |
| human endonuclease III homologue 1 (hNTH1) | Homo sapiens | 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex)EDTA instead of magnesium (EDTA concentration not stated) | 6.7 × 10⁻⁴ | 5 × 10⁻⁸ | 1.3 × 10⁴ calc. | pH 7.5, 37 °C | PMID 11380260 | Open record: human endonuclease III homologue 1 (hNTH1), 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex) |
| human endonuclease III homologue 1 (hNTH1)variant +G212 | Homo sapiens | 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex)2 mM MgCl2 (magnesium) | 3.5 × 10⁻⁵ | 1.7 × 10⁻⁶ | 21 calc. | pH 7.5, 37 °C | PMID 11380260 | Open record: human endonuclease III homologue 1 (hNTH1) variant +G212, 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex) |
| human endonuclease III homologue 1 (hNTH1)variant +G212 | Homo sapiens | 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex)EDTA instead of magnesium (EDTA concentration not stated) | 6.7 × 10⁻⁵ | 3.5 × 10⁻⁶ | 19 calc. | pH 7.5, 37 °C | PMID 11380260 | Open record: human endonuclease III homologue 1 (hNTH1) variant +G212, 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex) |
| human endonuclease III homologue 1 (hNTH1)variant +G212 | Homo sapiens | 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex)2 mM MgCl2 (magnesium) | 2.3 × 10⁻⁴ | 1.2 × 10⁻⁶ | 190 calc. | pH 7.5, 37 °C | PMID 11380260 | Open record: human endonuclease III homologue 1 (hNTH1) variant +G212, 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex) |
| human endonuclease III homologue 1 (hNTH1)variant +G212 | Homo sapiens | 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex)EDTA instead of magnesium (EDTA concentration not stated) | 1.17 × 10⁻⁴ | 1 × 10⁻⁶ | 120 calc. | pH 7.5, 37 °C | PMID 11380260 | Open record: human endonuclease III homologue 1 (hNTH1) variant +G212, 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex) |
| human endonuclease III homologue 1 (hNTH1)variant P211R | Homo sapiens | 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex)2 mM MgCl2 (magnesium) | 1 × 10⁻⁴ | 1.4 × 10⁻⁷ | 710 calc. | pH 7.5, 37 °C | PMID 11380260 | Open record: human endonuclease III homologue 1 (hNTH1) variant P211R, 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex) |
| human endonuclease III homologue 1 (hNTH1)variant P211R | Homo sapiens | 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex)EDTA instead of magnesium (EDTA concentration not stated) | 5 × 10⁻⁴ | 2 × 10⁻⁷ | 2.5 × 10³ calc. | pH 7.5, 37 °C | PMID 11380260 | Open record: human endonuclease III homologue 1 (hNTH1) variant P211R, 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with adenine (5ohC-A duplex) |
| human endonuclease III homologue 1 (hNTH1)variant P211R | Homo sapiens | 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex)2 mM MgCl2 (magnesium) | 5 × 10⁻⁴ | 4.8 × 10⁻⁸ | 1.0 × 10⁴ calc. | pH 7.5, 37 °C | PMID 11380260 | Open record: human endonuclease III homologue 1 (hNTH1) variant P211R, 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex) |
| human endonuclease III homologue 1 (hNTH1)variant P211R | Homo sapiens | 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex)EDTA instead of magnesium (EDTA concentration not stated) | 5.8 × 10⁻⁴ | 9 × 10⁻⁸ | 6.5 × 10³ calc. | pH 7.5, 37 °C | PMID 11380260 | Open record: human endonuclease III homologue 1 (hNTH1) variant P211R, 32P-end-labeled 40-mer DNA duplex containing 5-hydroxycytosine paired with guanine (5ohC-G duplex) |