CatLogenzyme kinetics catalog

Values CatLog read from this paper

Catalytic mechanism of Escherichia coli endonuclease VIII: roles of the intercalation loop and the zinc finger

PMID 17002303doi:10.1021/bi060663eOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 75 records (143 values) from this paper: endonuclease VIII (Nei) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.2.99.18

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), adenine (A) opposite the lesion5.8 × 10⁻⁴1.6 × 10⁻⁴3.7pH 7.5, 25 °COpen
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), cytosine (C) opposite the lesion0.00826.6 × 10⁻⁶1.23 × 10³pH 7.5, 25 °COpen
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), guanine (G) opposite the lesion0.0728.5 × 10⁻⁶8.5 × 10³pH 7.5, 25 °COpen
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), thymine (T) opposite the lesion0.00323.5 × 10⁻⁶900pH 7.5, 25 °COpen
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), adenine (A) opposite the lesion0.00786.1 × 10⁻⁴13pH 7.5, 25 °COpen
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), cytosine (C) opposite the lesion0.01586.8 × 10⁻⁴23pH 7.5, 25 °COpen
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), guanine (G) opposite the lesion0.01325.8 × 10⁻⁴23pH 7.5, 25 °COpen
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), thymine (T) opposite the lesion0.0230.001318pH 7.5, 25 °COpen
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, adenine (A) opposite the lesion0.01078.3 × 10⁻⁸1.28 × 10⁵pH 7.5, 25 °COpen
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, guanine (G) opposite the lesion0.00881.1 × 10⁻⁷8 × 10⁴pH 7.5, 25 °COpen
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), adenine (A) opposite the lesion0.0353.2 × 10⁻⁶1.1 × 10⁴pH 7.5, 25 °COpen
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), cytosine (C) opposite the lesion0.0424.4 × 10⁻⁶9.5 × 10³pH 7.5, 25 °COpen
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), guanine (G) opposite the lesion0.0332.3 × 10⁻⁶1.45 × 10⁴pH 7.5, 25 °COpen
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), thymine (T) opposite the lesion0.0484.4 × 10⁻⁶1.1 × 10⁴pH 7.5, 25 °COpen
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), adenine (A) opposite the lesion0.0231.6 × 10⁻⁵1.47 × 10³pH 7.5, 25 °COpen
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), cytosine (C) opposite the lesion0.01154.7 × 10⁻⁶2.5 × 10³pH 7.5, 25 °COpen
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), guanine (G) opposite the lesion0.0572.3 × 10⁻⁶2.5 × 10⁴pH 7.5, 25 °COpen
endonuclease VIII (Nei)Escherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), thymine (T) opposite the lesion0.01124.6 × 10⁻⁶2.5 × 10³pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q261AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), adenine (A) opposite the lesion2.3 × 10⁻⁴2.6 × 10⁻⁴0.9pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q261AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), cytosine (C) opposite the lesion1.3 × 10⁻⁴2.7 × 10⁻⁴0.5pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q261AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), guanine (G) opposite the lesion1.5 × 10⁻⁴7.7 × 10⁻⁵2pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q261AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), thymine (T) opposite the lesion6.7 × 10⁻⁵0.00140.048pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q261AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), adenine (A) opposite the lesion——3.3 × 10⁻⁴pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q261AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), cytosine (C) opposite the lesion——7.2 × 10⁻⁴pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q261AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), guanine (G) opposite the lesion——6.3 × 10⁻⁴pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q261AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), thymine (T) opposite the lesion——2.3 × 10⁻⁴pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q261AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), adenine (A) opposite the lesion——3pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q261AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), cytosine (C) opposite the lesion——2.8pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q261AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), guanine (G) opposite the lesion0.00582.4 × 10⁻⁴25pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q261AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), thymine (T) opposite the lesion——2.8pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q261AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), adenine (A) opposite the lesion——3pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q261AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), cytosine (C) opposite the lesion——3.2pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q261AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), guanine (G) opposite the lesion0.00572.1 × 10⁻⁴27pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q261AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), thymine (T) opposite the lesion——3.3pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), adenine (A) opposite the lesion7.2 × 10⁻⁵3.8 × 10⁻⁵1.8pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), cytosine (C) opposite the lesion0.01331.4 × 10⁻⁴95pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), guanine (G) opposite the lesion6.5 × 10⁻⁵4.2 × 10⁻⁵1.55pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), thymine (T) opposite the lesion0.01171.6 × 10⁻⁴73pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), adenine (A) opposite the lesion——0.077pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), cytosine (C) opposite the lesion——0.11pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), guanine (G) opposite the lesion——0.097pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), thymine (T) opposite the lesion——0.092pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, adenine (A) opposite the lesion0.00152.8 × 10⁻⁵53pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, guanine (G) opposite the lesion0.00182.2 × 10⁻⁵83pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), adenine (A) opposite the lesion——78pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), cytosine (C) opposite the lesion——92pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), guanine (G) opposite the lesion0.0164.9 × 10⁻⁵330pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), thymine (T) opposite the lesion0.0221.9 × 10⁻⁴113pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), adenine (A) opposite the lesion——98pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), cytosine (C) opposite the lesion——93pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), guanine (G) opposite the lesion——90pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant Q69A/L70A/Y71AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), thymine (T) opposite the lesion——90pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant R171AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), adenine (A) opposite the lesion——0.062pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant R171AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), cytosine (C) opposite the lesion——0.018pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant R171AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), guanine (G) opposite the lesion——0.028pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant R171AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 8-oxo-7,8-dihydroguanine (8-oxoG), thymine (T) opposite the lesion——0.048pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant R171AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, adenine (A) opposite the lesion——0.017pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant R171AEscherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = abasic (AP) site made from uracil by uracil-DNA glycosylase, guanine (G) opposite the lesion——0.01pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant R171AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), adenine (A) opposite the lesion——0.085pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant R171AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), cytosine (C) opposite the lesion——0.047pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant R171AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), guanine (G) opposite the lesion——0.073pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant R171AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), thymine (T) opposite the lesion——0.088pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant R171AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), adenine (A) opposite the lesion——0.043pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant R171AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), cytosine (C) opposite the lesion——0.028pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant R171AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), guanine (G) opposite the lesion——0.035pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant R171AEscherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), thymine (T) opposite the lesion——0.05pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant ΔQLY69-71Escherichia coli23-mer DNA duplex 5'-CTCTCCCTTCXCTCCTTTCCTCT-3' with X = 5,6-dihydrouracil (DHU), guanine (G) opposite the lesion4.8 × 10⁻⁵5.4 × 10⁻⁵0.9pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant ΔQLY69-71Escherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), adenine (A) opposite the lesion0.00256.6 × 10⁻⁵38pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant ΔQLY69-71Escherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), cytosine (C) opposite the lesion——48pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant ΔQLY69-71Escherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), guanine (G) opposite the lesion——52pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant ΔQLY69-71Escherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5R,6S)-thymine glycol (TgC), thymine (T) opposite the lesion——47pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant ΔQLY69-71Escherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), adenine (A) opposite the lesion——62pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant ΔQLY69-71Escherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), cytosine (C) opposite the lesion——62pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant ΔQLY69-71Escherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), guanine (G) opposite the lesion——62pH 7.5, 25 °COpen
endonuclease VIII (Nei)variant ΔQLY69-71Escherichia coli23-mer DNA duplex 5'-GACAAGCGCAGYCAGCCGAACAC-3' with Y = cis-(5S,6R)-thymine glycol (TgA), thymine (T) opposite the lesion——62pH 7.5, 25 °COpen