Values CatLog read from this paper
Structure prediction and active site analysis of the metal binding determinants in gamma -glutamylcysteine synthetase
PMID 11527962doi:10.1074/jbc.m104672200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 22 records (32 values) from this paper: γ-glutamylcysteine synthetase (γ-GCS) from Trypanosoma brucei.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| γ-glutamylcysteine synthetase (γ-GCS) | Trypanosoma brucei | L-alpha-aminobutyrate | — | 6 | — | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS), L-alpha-aminobutyrate |
| γ-glutamylcysteine synthetase (γ-GCS) | Trypanosoma brucei | L-glutamate | — | 1 | — | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS), L-glutamate |
| γ-glutamylcysteine synthetase (γ-GCS) | Trypanosoma brucei | MgATP | 3.8 | — | — | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS), MgATP |
| γ-glutamylcysteine synthetase (γ-GCS) | Trypanosoma brucei | MnATPMn2+ as activating metal | 2.4 | 0.022 | 110 | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS), MnATP |
| γ-glutamylcysteine synthetase (γ-GCS) | Trypanosoma brucei | MnATPenzyme dialyzed free of divalent metal; fixed L-Glu 10 mM | 2.5 | — | — | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS), MnATP |
| γ-glutamylcysteine synthetase (γ-GCS)variant E489A | Trypanosoma brucei | L-alpha-aminobutyrateMg2+ as activating metal; MgATP | — | 15 | — | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E489A, L-alpha-aminobutyrate |
| γ-glutamylcysteine synthetase (γ-GCS)variant E489A | Trypanosoma brucei | L-alpha-aminobutyrateMn2+ as activating metal; free Mn2+ held constant at 1.7 mM | — | 9.4 | — | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E489A, L-alpha-aminobutyrate |
| γ-glutamylcysteine synthetase (γ-GCS)variant E489A | Trypanosoma brucei | L-glutamateMg2+ as activating metal; MgATP | — | 1.1 | — | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E489A, L-glutamate |
| γ-glutamylcysteine synthetase (γ-GCS)variant E489A | Trypanosoma brucei | L-glutamateMn2+ as activating metal; free Mn2+ held constant at 1.7 mM | — | 0.65 | — | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E489A, L-glutamate |
| γ-glutamylcysteine synthetase (γ-GCS)variant E489A | Trypanosoma brucei | MgATPMg2+ as activating metal | 0.0053 | 2 | 0.0027 | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E489A, MgATP |
| γ-glutamylcysteine synthetase (γ-GCS)variant E489A | Trypanosoma brucei | MgATPenzyme dialyzed free of divalent metal; fixed L-Glu 10 mM | 0.0066 | — | — | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E489A, MgATP |
| γ-glutamylcysteine synthetase (γ-GCS)variant E489A | Trypanosoma brucei | MnATP | 0.89 | 0.1 | 8.1 | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E489A, MnATP |
| γ-glutamylcysteine synthetase (γ-GCS)variant E93A | Trypanosoma brucei | L-glutamate | — | 11 | — | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E93A, L-glutamate |
| γ-glutamylcysteine synthetase (γ-GCS)variant E93A | Trypanosoma brucei | MgATPMg2+ as activating metal; (100 mM), L-Aba | 0.066 | 0.32 | 0.21 | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E93A, MgATP |
| γ-glutamylcysteine synthetase (γ-GCS)variant E93A | Trypanosoma brucei | MgATPenzyme dialyzed free of divalent metal; fixed L-Glu 10 mM | 0.078 | — | — | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E93A, MgATP |
| γ-glutamylcysteine synthetase (γ-GCS)variant E93A | Trypanosoma brucei | MnATP | 0.089 | — | — | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E93A, MnATP |
| γ-glutamylcysteine synthetase (γ-GCS)variant Q321A | Trypanosoma brucei | L-alpha-aminobutyrateMg2+ as activating metal; MgATP (15 mM ATP) | — | 18 | — | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant Q321A, L-alpha-aminobutyrate |
| γ-glutamylcysteine synthetase (γ-GCS)variant Q321A | Trypanosoma brucei | L-alpha-aminobutyrateMn2+ as activating metal; free Mn2+ held constant at 2 mM | — | 14 | — | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant Q321A, L-alpha-aminobutyrate |
| γ-glutamylcysteine synthetase (γ-GCS)variant Q321A | Trypanosoma brucei | L-glutamateMg2+ as activating metal; MgATP (15 mM ATP) | — | 1.6 | — | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant Q321A, L-glutamate |
| γ-glutamylcysteine synthetase (γ-GCS)variant Q321A | Trypanosoma brucei | L-glutamateMn2+ as activating metal; free Mn2+ held constant at 2 mM | — | 1.1 | — | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant Q321A, L-glutamate |
| γ-glutamylcysteine synthetase (γ-GCS)variant Q321A | Trypanosoma brucei | MgATP | — | — | 0.0037 | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant Q321A, MgATP |
| γ-glutamylcysteine synthetase (γ-GCS)variant Q321A | Trypanosoma brucei | MnATP | 1.5 | 1.2 | 1.3 | pH 8, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant Q321A, MnATP |