EC 6.3.2.2: glutamate-cysteine ligase

LigasesOpen in Browse

CatLog read 154 records for EC 6.3.2.2 from 10 papers, for 7 organisms. Names in these papers: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), γ-glutamylcysteine synthetase (γ-GCS), γ-glutamylcysteine synthetase, glutamate-cysteine ligase (SynGCL) and 5 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 212 records for EC 6.3.2.2 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.3.2.2 in Browse.

Papers

Papers with records for EC 6.3.2.2
Paper	Organisms	Records
Escherichia coli gamma-glutamylcysteine synthetase. Two active site metal ions affect substrate and inhibitor binding	Escherichia coli	46
Structure prediction and active site analysis of the metal binding determinants in gamma -glutamylcysteine synthetase	Trypanosoma brucei	22
Redox-dependent stability of the γ-glutamylcysteine synthetase enzyme of Escherichia coli: a novel means of redox regulation	Escherichia coli	21
Substrate binding determinants of Trypanosoma brucei gamma-glutamylcysteine synthetase	Trypanosoma brucei	20
Probing the origins of glutathione biosynthesis through biochemical analysis of glutamate-cysteine ligase and glutathione synthetase from a model photosynthetic prokaryote	Synechocystis sp. PCC 6803	17
Characterization of glutamate-cysteine ligase and glutathione synthetase from the δ-proteobacterium Myxococcus xanthus	Myxococcus xanthus	13
Characterization of Trypanosoma brucei gamma-glutamylcysteine synthetase, an essential enzyme in the biosynthesis of trypanothione (diglutathionylspermidine)	Trypanosoma brucei	5
Purification and characterization of gamma-glutamylcysteine synthetase from Ascaris suum	Ascaris suum	4
Arabidopsis thaliana glutamate-cysteine ligase: functional properties, kinetic mechanism, and regulation of activity	Arabidopsis thaliana	3
Characterization of the bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase (GshF) of Pasteurella multocida	Pasteurella multocida	3

Records

Records for EC 6.3.2.2 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
gamma-glutamylcysteine synthetase	Ascaris suum	adenosine 5'-triphosphate	—	1.41	—	pH 8, 37 °C	PMID 8538700	Open record: gamma-glutamylcysteine synthetase, adenosine 5'-triphosphate
gamma-glutamylcysteine synthetase	Ascaris suum	L-2-aminobutyrate	—	0.31	—	pH 8, 37 °C	PMID 8538700	Open record: gamma-glutamylcysteine synthetase, L-2-aminobutyrate
gamma-glutamylcysteine synthetase	Ascaris suum	L-cysteine	—	0.41	—	pH 8, 37 °C	PMID 8538700	Open record: gamma-glutamylcysteine synthetase, L-cysteine
gamma-glutamylcysteine synthetase	Ascaris suum	L-glutamate	—	0.94	—	pH 8, 37 °C	PMID 8538700	Open record: gamma-glutamylcysteine synthetase, L-glutamate
gamma-glutamylcysteine synthetase (catalytic subunit)	Trypanosoma brucei	adenosine 5'-triphosphate	—	0.071	—	pH 8, 37 °C	PMID 8663359	Open record: gamma-glutamylcysteine synthetase (catalytic subunit), adenosine 5'-triphosphate
gamma-glutamylcysteine synthetase (catalytic subunit)	Trypanosoma brucei	L-2-aminobutanoic acid	—	10.4	—	pH 8, 37 °C	PMID 8663359	Open record: gamma-glutamylcysteine synthetase (catalytic subunit), L-2-aminobutanoic acid
gamma-glutamylcysteine synthetase (catalytic subunit)	Trypanosoma brucei	L-cysteine	—	0.69	—	pH 8, 37 °C	PMID 8663359	Open record: gamma-glutamylcysteine synthetase (catalytic subunit), L-cysteine
gamma-glutamylcysteine synthetase (catalytic subunit)	Trypanosoma brucei	L-glutamateacid and ATP (saturating)	—	0.24	—	pH 8, 37 °C	PMID 8663359	Open record: gamma-glutamylcysteine synthetase (catalytic subunit), L-glutamate
gamma-glutamylcysteine synthetase (catalytic subunit)	Trypanosoma brucei	L-glutamatespecific activity assay at 5 mM ATP; 10 mM L-Glu; acid, ATP	9.8	—	—	pH 8, 37 °C	PMID 8663359	Open record: gamma-glutamylcysteine synthetase (catalytic subunit), L-glutamate
glutamate-cysteine ligase (AtGCL)	Arabidopsis thaliana	adenosine 5'-triphosphate	0.113	2.7	0.042	pH 7, 25 °C	PMID 15180996	Open record: glutamate-cysteine ligase (AtGCL), adenosine 5'-triphosphate
glutamate-cysteine ligase (AtGCL)	Arabidopsis thaliana	L-cysteine	0.075	1.6	0.0469	pH 7, 25 °C	PMID 15180996	Open record: glutamate-cysteine ligase (AtGCL), L-cysteine
glutamate-cysteine ligase (AtGCL)	Arabidopsis thaliana	L-glutamate	0.102	9.1	0.0101	pH 7, 25 °C	PMID 15180996	Open record: glutamate-cysteine ligase (AtGCL), L-glutamate
glutamate-cysteine ligase (SynGCL)	Synechocystis sp. PCC 6803	ATP	0.51	0.145	3.52	pH 7.5, 25 °C	PMID 23170977	Open record: glutamate-cysteine ligase (SynGCL), ATP
glutamate-cysteine ligase (SynGCL)	Synechocystis sp. PCC 6803	Cysteine	0.42	0.059	7.12	pH 7.5, 25 °C	PMID 23170977	Open record: glutamate-cysteine ligase (SynGCL), Cysteine
glutamate-cysteine ligase (SynGCL)	Synechocystis sp. PCC 6803	Glutamate	0.35	0.953	0.367	pH 7.5, 25 °C	PMID 23170977	Open record: glutamate-cysteine ligase (SynGCL), Glutamate
glutamate-cysteine ligase (SynGCL)variant H121A	Synechocystis sp. PCC 6803	ATP	0.098	0.31	0.317	pH 7.5, 25 °C	PMID 23170977	Open record: glutamate-cysteine ligase (SynGCL) variant H121A, ATP
glutamate-cysteine ligase (SynGCL)variant H121A	Synechocystis sp. PCC 6803	Cysteine	0.09	0.707	0.127	pH 7.5, 25 °C	PMID 23170977	Open record: glutamate-cysteine ligase (SynGCL) variant H121A, Cysteine
glutamate-cysteine ligase (SynGCL)variant H121A	Synechocystis sp. PCC 6803	Glutamate	0.127	15.2	0.0083	pH 7.5, 25 °C	PMID 23170977	Open record: glutamate-cysteine ligase (SynGCL) variant H121A, Glutamate
glutamate-cysteine ligase (SynGCL)variant H121Q	Synechocystis sp. PCC 6803	ATP	0.382	0.279	1.37	pH 7.5, 25 °C	PMID 23170977	Open record: glutamate-cysteine ligase (SynGCL) variant H121Q, ATP
glutamate-cysteine ligase (SynGCL)variant H121Q	Synechocystis sp. PCC 6803	Cysteine	0.335	0.116	2.89	pH 7.5, 25 °C	PMID 23170977	Open record: glutamate-cysteine ligase (SynGCL) variant H121Q, Cysteine
glutamate-cysteine ligase (SynGCL)variant H121Q	Synechocystis sp. PCC 6803	Glutamate	0.188	1.18	0.16	pH 7.5, 25 °C	PMID 23170977	Open record: glutamate-cysteine ligase (SynGCL) variant H121Q, Glutamate
glutamate-cysteine ligase (SynGCL)variant R167K	Synechocystis sp. PCC 6803	ATP	0.038	0.445	0.0861	pH 7.5, 25 °C	PMID 23170977	Open record: glutamate-cysteine ligase (SynGCL) variant R167K, ATP
glutamate-cysteine ligase (SynGCL)variant R167K	Synechocystis sp. PCC 6803	Cysteine	0.025	1.95	0.0128	pH 7.5, 25 °C	PMID 23170977	Open record: glutamate-cysteine ligase (SynGCL) variant R167K, Cysteine
glutamate-cysteine ligase (SynGCL)variant R248K	Synechocystis sp. PCC 6803	ATP	0.0083	0.969	0.0086	pH 7.5, 25 °C	PMID 23170977	Open record: glutamate-cysteine ligase (SynGCL) variant R248K, ATP
glutamate-cysteine ligase (SynGCL)variant R248K	Synechocystis sp. PCC 6803	Cysteine	0.01	0.857	0.0117	pH 7.5, 25 °C	PMID 23170977	Open record: glutamate-cysteine ligase (SynGCL) variant R248K, Cysteine
glutamate-cysteine ligase (SynGCL)variant R248K	Synechocystis sp. PCC 6803	Glutamate	0.0083	3.76	0.0022	pH 7.5, 25 °C	PMID 23170977	Open record: glutamate-cysteine ligase (SynGCL) variant R248K, Glutamate
glutamate-cysteine ligase (SynGCL)variant T117S	Synechocystis sp. PCC 6803	ATP	0.225	0.352	0.639	pH 7.5, 25 °C	PMID 23170977	Open record: glutamate-cysteine ligase (SynGCL) variant T117S, ATP
glutamate-cysteine ligase (SynGCL)variant T117S	Synechocystis sp. PCC 6803	Cysteine	0.133	0.186	0.717	pH 7.5, 25 °C	PMID 23170977	Open record: glutamate-cysteine ligase (SynGCL) variant T117S, Cysteine
glutamate-cysteine ligase (SynGCL)variant T117S	Synechocystis sp. PCC 6803	Glutamate	0.137	6.1	0.0224	pH 7.5, 25 °C	PMID 23170977	Open record: glutamate-cysteine ligase (SynGCL) variant T117S, Glutamate
GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase)	Pasteurella multocida	adenosine 5'-triphosphate	22.4	0.25	89.6	pH 8.2, 25 °C	PMID 16339152	Open record: GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase), adenosine 5'-triphosphate
GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase)	Pasteurella multocida	L-cysteine	25.3	0.22	115	pH 8.2, 25 °C	PMID 16339152	Open record: GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase), L-cysteine
GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase)	Pasteurella multocida	L-glutamate	24.3	5.3	4.58	pH 8.2, 25 °C	PMID 16339152	Open record: GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase), L-glutamate
mxGcl2 (glutamate-cysteine ligase)	Myxococcus xanthus	adenosine 5'-triphosphate	2.01	0.88	2.28	pH 7, 37 °C	PMID 35277888	Open record: mxGcl2 (glutamate-cysteine ligase), adenosine 5'-triphosphate
mxGcl2 (glutamate-cysteine ligase)	Myxococcus xanthus	L-cysteine	1.11	1.41	0.79	pH 7, 37 °C	PMID 35277888	Open record: mxGcl2 (glutamate-cysteine ligase), L-cysteine
mxGcl2 (glutamate-cysteine ligase)	Myxococcus xanthus	L-glutamate	0.67	0.68	0.99	pH 7, 37 °C	PMID 35277888	Open record: mxGcl2 (glutamate-cysteine ligase), L-glutamate
mxGcl2 (glutamate-cysteine ligase)	Myxococcus xanthus	α-aminobutyrate (2-aminobutanoate)	1.42	15.7	0.09	pH 7, 37 °C	PMID 35277888	Open record: mxGcl2 (glutamate-cysteine ligase), α-aminobutyrate (2-aminobutanoate)
mxGcl2 (glutamate-cysteine ligase)variant F308L	Myxococcus xanthus	adenosine 5'-triphosphate	0.49	1.07	0.46	pH 7, 37 °C	PMID 35277888	Open record: mxGcl2 (glutamate-cysteine ligase) variant F308L, adenosine 5'-triphosphate
mxGcl2 (glutamate-cysteine ligase)variant F308L	Myxococcus xanthus	L-cysteine	0.45	2.7	0.17	pH 7, 37 °C	PMID 35277888	Open record: mxGcl2 (glutamate-cysteine ligase) variant F308L, L-cysteine
mxGcl2 (glutamate-cysteine ligase)variant F308L	Myxococcus xanthus	L-glutamate	0.5	0.96	0.52	pH 7, 37 °C	PMID 35277888	Open record: mxGcl2 (glutamate-cysteine ligase) variant F308L, L-glutamate
mxGcl2 (glutamate-cysteine ligase)variant L267M	Myxococcus xanthus	adenosine 5'-triphosphate	0.61	0.78	0.78	pH 7, 37 °C	PMID 35277888	Open record: mxGcl2 (glutamate-cysteine ligase) variant L267M, adenosine 5'-triphosphate
mxGcl2 (glutamate-cysteine ligase)variant L267M	Myxococcus xanthus	L-cysteine	0.78	0.45	1.73	pH 7, 37 °C	PMID 35277888	Open record: mxGcl2 (glutamate-cysteine ligase) variant L267M, L-cysteine
mxGcl2 (glutamate-cysteine ligase)variant L267M	Myxococcus xanthus	L-glutamate	1.03	0.68	1.51	pH 7, 37 °C	PMID 35277888	Open record: mxGcl2 (glutamate-cysteine ligase) variant L267M, L-glutamate
mxGcl2 (glutamate-cysteine ligase)variant W233F	Myxococcus xanthus	adenosine 5'-triphosphate	3.81	1.68	2.27	pH 7, 37 °C	PMID 35277888	Open record: mxGcl2 (glutamate-cysteine ligase) variant W233F, adenosine 5'-triphosphate
mxGcl2 (glutamate-cysteine ligase)variant W233F	Myxococcus xanthus	L-cysteine	4.61	1.91	2.41	pH 7, 37 °C	PMID 35277888	Open record: mxGcl2 (glutamate-cysteine ligase) variant W233F, L-cysteine
mxGcl2 (glutamate-cysteine ligase)variant W233F	Myxococcus xanthus	L-glutamate	4.97	3.12	1.59	pH 7, 37 °C	PMID 35277888	Open record: mxGcl2 (glutamate-cysteine ligase) variant W233F, L-glutamate
γ-glutamylcysteine synthetase	Trypanosoma brucei	gamma-aminobutyric acid	0.14	150	9.3 × 10⁻⁴ calc.	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase, gamma-aminobutyric acid
γ-glutamylcysteine synthetasevariant R179A	Trypanosoma brucei	ATP	—	0.14	—	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant R179A, ATP
γ-glutamylcysteine synthetasevariant R179A	Trypanosoma brucei	L-alpha-aminobutyrate	—	380	—	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant R179A, L-alpha-aminobutyrate
γ-glutamylcysteine synthetasevariant R179A	Trypanosoma brucei	L-glutamate	0.61	5.2	0.12 calc.	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant R179A, L-glutamate
γ-glutamylcysteine synthetasevariant R366A	Trypanosoma brucei	ATP	—	2.8	—	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant R366A, ATP
γ-glutamylcysteine synthetasevariant R366A	Trypanosoma brucei	gamma-aminobutyric acid	0.59	20	0.029 calc.	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant R366A, gamma-aminobutyric acid
γ-glutamylcysteine synthetasevariant R366A	Trypanosoma brucei	L-alpha-aminobutyrate	—	34	—	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant R366A, L-alpha-aminobutyrate
γ-glutamylcysteine synthetasevariant R366A	Trypanosoma brucei	L-glutamate	0.32	130	0.0025 calc.	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant R366A, L-glutamate
γ-glutamylcysteine synthetasevariant R474A	Trypanosoma brucei	ATP	—	1.3	—	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant R474A, ATP
γ-glutamylcysteine synthetasevariant R474A	Trypanosoma brucei	L-alpha-aminobutyrate	—	56	—	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant R474A, L-alpha-aminobutyrate
γ-glutamylcysteine synthetasevariant R474A	Trypanosoma brucei	L-glutamate	0.24	1.6	0.15 calc.	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant R474A, L-glutamate
γ-glutamylcysteine synthetasevariant R487A	Trypanosoma brucei	ATP	—	1.2	—	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant R487A, ATP
γ-glutamylcysteine synthetasevariant R487A	Trypanosoma brucei	L-alpha-aminobutyrate	—	15	—	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant R487A, L-alpha-aminobutyrate
γ-glutamylcysteine synthetasevariant R487A	Trypanosoma brucei	L-glutamate	1.2	1.3	0.92 calc.	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant R487A, L-glutamate
γ-glutamylcysteine synthetasevariant R491A	Trypanosoma brucei	ATP	—	0.11	—	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant R491A, ATP
γ-glutamylcysteine synthetasevariant R491A	Trypanosoma brucei	L-glutamate	0.055	0.61	0.090 calc.	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant R491A, L-glutamate
γ-glutamylcysteine synthetasevariant T323A	Trypanosoma brucei	ATP	—	7	—	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant T323A, ATP
γ-glutamylcysteine synthetasevariant T323A	Trypanosoma brucei	L-alpha-aminobutyrate	—	7.5	—	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant T323A, L-alpha-aminobutyrate
γ-glutamylcysteine synthetasevariant T323A	Trypanosoma brucei	L-glutamate20 mM total MgCl2	0.79	1.7	0.46 calc.	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant T323A, L-glutamate
γ-glutamylcysteine synthetasevariant T323A	Trypanosoma brucei	L-glutamateMnCl2 instead of MgCl2; substrates at the standard 5 mM ATP	6	—	—	37 °C	PMID 11851421	Open record: γ-glutamylcysteine synthetase variant T323A, L-glutamate
γ-glutamylcysteine synthetase (γ-GCS)	Escherichia coli	adenosine 5'-triphosphateTable 2	46.9	0.14	335	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS), adenosine 5'-triphosphate
γ-glutamylcysteine synthetase (γ-GCS)	Escherichia coli	adenosine 5'-triphosphateTable 3	28.7	0.46	62.4	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS), adenosine 5'-triphosphate
γ-glutamylcysteine synthetase (γ-GCS)	Escherichia coli	L-cysteineTable 2	46.6	0.1	466	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS), L-cysteine
γ-glutamylcysteine synthetase (γ-GCS)	Escherichia coli	L-cysteineTable 3	24.2	0.14	172.9	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS), L-cysteine
γ-glutamylcysteine synthetase (γ-GCS)	Escherichia coli	L-glutamic acidTable 2	31.4	3.9	8.1	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS), L-glutamic acid
γ-glutamylcysteine synthetase (γ-GCS)	Escherichia coli	L-glutamic acidTable 3	22.1	5.1	4.3	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS), L-glutamic acid
γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C372S/R374Q/V375F/C395S/C433S/C439S	Escherichia coli	adenosine 5'-triphosphate	27.2	0.19	142.9	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C372S/R374Q/V375F/C395S/C433S/C439S, adenosine 5'-triphosphate
γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C372S/R374Q/V375F/C395S/C433S/C439S	Escherichia coli	L-cysteine	29.2	0.14	208.6	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C372S/R374Q/V375F/C395S/C433S/C439S, L-cysteine
γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C372S/R374Q/V375F/C395S/C433S/C439S	Escherichia coli	L-glutamic acid	29.6	4	7.4	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C372S/R374Q/V375F/C395S/C433S/C439S, L-glutamic acid
γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C395W/C433S/C439S	Escherichia coli	adenosine 5'-triphosphate	30.5	0.24	127.1	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C395W/C433S/C439S, adenosine 5'-triphosphate
γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C395W/C433S/C439S	Escherichia coli	L-cysteine	31.8	0.16	198.8	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C395W/C433S/C439S, L-cysteine
γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C395W/C433S/C439S	Escherichia coli	L-glutamic acid	30.4	4.1	7.4	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C395W/C433S/C439S, L-glutamic acid
γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C395Y/C433S/C439S	Escherichia coli	adenosine 5'-triphosphate	24.7	0.53	46.6	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C395Y/C433S/C439S, adenosine 5'-triphosphate
γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C395Y/C433S/C439S	Escherichia coli	L-cysteine	21.9	0.11	199.1	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C395Y/C433S/C439S, L-cysteine
γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C395Y/C433S/C439S	Escherichia coli	L-glutamic acid	22.1	3.2	6.9	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C395Y/C433S/C439S, L-glutamic acid
γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C433S/C439S	Escherichia coli	adenosine 5'-triphosphateTable 2	39.7	0.1	397	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C433S/C439S, adenosine 5'-triphosphate
γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C433S/C439S	Escherichia coli	adenosine 5'-triphosphateTable 3	30.1	0.25	120.4	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C433S/C439S, adenosine 5'-triphosphate
γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C433S/C439S	Escherichia coli	L-cysteineTable 2	39.7	0.11	396.9	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C433S/C439S, L-cysteine
γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C433S/C439S	Escherichia coli	L-cysteineTable 3	22.2	0.1	222	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C433S/C439S, L-cysteine
γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C433S/C439S	Escherichia coli	L-glutamic acidTable 2	34	4	8.5	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C433S/C439S, L-glutamic acid
γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C433S/C439S	Escherichia coli	L-glutamic acidTable 3	23.8	5.3	4.5	pH 8, 25 °C	PMID 23126248	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C433S/C439S, L-glutamic acid
γ-glutamylcysteine synthetase (γ-GCS)	Trypanosoma brucei	L-alpha-aminobutyrate	—	6	—	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS), L-alpha-aminobutyrate
γ-glutamylcysteine synthetase (γ-GCS)	Trypanosoma brucei	L-glutamate	—	1	—	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS), L-glutamate
γ-glutamylcysteine synthetase (γ-GCS)	Trypanosoma brucei	MgATP	3.8	—	—	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS), MgATP
γ-glutamylcysteine synthetase (γ-GCS)	Trypanosoma brucei	MnATPMn2+ as activating metal	2.4	0.022	110	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS), MnATP
γ-glutamylcysteine synthetase (γ-GCS)	Trypanosoma brucei	MnATPenzyme dialyzed free of divalent metal; fixed L-Glu 10 mM	2.5	—	—	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS), MnATP
γ-glutamylcysteine synthetase (γ-GCS)variant E489A	Trypanosoma brucei	L-alpha-aminobutyrateMg2+ as activating metal; MgATP	—	15	—	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E489A, L-alpha-aminobutyrate
γ-glutamylcysteine synthetase (γ-GCS)variant E489A	Trypanosoma brucei	L-alpha-aminobutyrateMn2+ as activating metal; free Mn2+ held constant at 1.7 mM	—	9.4	—	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E489A, L-alpha-aminobutyrate
γ-glutamylcysteine synthetase (γ-GCS)variant E489A	Trypanosoma brucei	L-glutamateMg2+ as activating metal; MgATP	—	1.1	—	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E489A, L-glutamate
γ-glutamylcysteine synthetase (γ-GCS)variant E489A	Trypanosoma brucei	L-glutamateMn2+ as activating metal; free Mn2+ held constant at 1.7 mM	—	0.65	—	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E489A, L-glutamate
γ-glutamylcysteine synthetase (γ-GCS)variant E489A	Trypanosoma brucei	MgATPMg2+ as activating metal	0.0053	2	0.0027	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E489A, MgATP
γ-glutamylcysteine synthetase (γ-GCS)variant E489A	Trypanosoma brucei	MgATPenzyme dialyzed free of divalent metal; fixed L-Glu 10 mM	0.0066	—	—	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E489A, MgATP
γ-glutamylcysteine synthetase (γ-GCS)variant E489A	Trypanosoma brucei	MnATP	0.89	0.1	8.1	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E489A, MnATP
γ-glutamylcysteine synthetase (γ-GCS)variant E93A	Trypanosoma brucei	L-glutamate	—	11	—	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E93A, L-glutamate
γ-glutamylcysteine synthetase (γ-GCS)variant E93A	Trypanosoma brucei	MgATPMg2+ as activating metal; (100 mM), L-Aba	0.066	0.32	0.21	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E93A, MgATP
γ-glutamylcysteine synthetase (γ-GCS)variant E93A	Trypanosoma brucei	MgATPenzyme dialyzed free of divalent metal; fixed L-Glu 10 mM	0.078	—	—	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E93A, MgATP
γ-glutamylcysteine synthetase (γ-GCS)variant E93A	Trypanosoma brucei	MnATP	0.089	—	—	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant E93A, MnATP
γ-glutamylcysteine synthetase (γ-GCS)variant Q321A	Trypanosoma brucei	L-alpha-aminobutyrateMg2+ as activating metal; MgATP (15 mM ATP)	—	18	—	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant Q321A, L-alpha-aminobutyrate
γ-glutamylcysteine synthetase (γ-GCS)variant Q321A	Trypanosoma brucei	L-alpha-aminobutyrateMn2+ as activating metal; free Mn2+ held constant at 2 mM	—	14	—	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant Q321A, L-alpha-aminobutyrate
γ-glutamylcysteine synthetase (γ-GCS)variant Q321A	Trypanosoma brucei	L-glutamateMg2+ as activating metal; MgATP (15 mM ATP)	—	1.6	—	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant Q321A, L-glutamate
γ-glutamylcysteine synthetase (γ-GCS)variant Q321A	Trypanosoma brucei	L-glutamateMn2+ as activating metal; free Mn2+ held constant at 2 mM	—	1.1	—	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant Q321A, L-glutamate
γ-glutamylcysteine synthetase (γ-GCS)variant Q321A	Trypanosoma brucei	MgATP	—	—	0.0037	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant Q321A, MgATP
γ-glutamylcysteine synthetase (γ-GCS)variant Q321A	Trypanosoma brucei	MnATP	1.5	1.2	1.3	pH 8, 37 °C	PMID 11527962	Open record: γ-glutamylcysteine synthetase (γ-GCS) variant Q321A, MnATP
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	D-glutamate25 mM MgCl2	—	75	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), D-glutamate
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	D-glutamate10 mM MnCl2	—	5.9	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), D-glutamate
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	glycine25 mM MgCl2	—	17.6	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), glycine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	glycine10 mM MnCl2	—	1.7	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), glycine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-2-aminobutanoate25 mM MgCl2	—	3.9	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-2-aminobutanoate
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-2-aminobutanoate10 mM MnCl2	—	0.41	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-2-aminobutanoate
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-2-aminopent-4-enoic acid25 mM MgCl2	—	6.9	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-2-aminopent-4-enoic acid
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-2-aminopent-4-enoic acid10 mM MnCl2	—	4.2	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-2-aminopent-4-enoic acid
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-alanine25 mM MgCl2	—	21.7	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-alanine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-alanine10 mM MnCl2	—	7.8	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-alanine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-allo-threonine25 mM MgCl2	—	18.8	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-allo-threonine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-allo-threonine10 mM MnCl2	—	4.4	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-allo-threonine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-cysteine25 mM MgCl2	—	0.1	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-cysteine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-cysteine10 mM MnCl2	—	0.045	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-cysteine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-glutamate25 mM MgCl2	—	1.9	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-glutamate
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-glutamate10 mM MnCl2	—	0.32	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-glutamate
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-isoleucine25 mM MgCl2	—	23	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-isoleucine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-isoleucine10 mM MnCl2	—	9.3	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-isoleucine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-leucine25 mM MgCl2	—	57.9	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-leucine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-leucine10 mM MnCl2	—	19	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-leucine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-norleucine25 mM MgCl2	—	33.3	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-norleucine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-norleucine10 mM MnCl2	—	12.6	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-norleucine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-norvaline25 mM MgCl2	—	61	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-norvaline
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-norvaline10 mM MnCl2	—	16.3	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-norvaline
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-serine25 mM MgCl2	—	24.6	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-serine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-serine10 mM MnCl2	—	7.6	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-serine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-threonine25 mM MgCl2	—	73.9	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-threonine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-threonine10 mM MnCl2	—	114	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-threonine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-valine25 mM MgCl2	—	27.1	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-valine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	L-valine10 mM MnCl2	—	21	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-valine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	N-methyl-L-glutamate25 mM MgCl2	—	27.6	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), N-methyl-L-glutamate
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	N-methyl-L-glutamate10 mM MnCl2	—	0.37	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), N-methyl-L-glutamate
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	O-methyl-D,L-serine25 mM MgCl2	—	31.1	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), O-methyl-D,L-serine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	O-methyl-D,L-serine10 mM MnCl2	—	6.1	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), O-methyl-D,L-serine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	S-methyl-L-cysteine25 mM MgCl2	—	8.1	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), S-methyl-L-cysteine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	S-methyl-L-cysteine10 mM MnCl2	—	0.32	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), S-methyl-L-cysteine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	α-ethyl-L-glutamate25 mM MgCl2	—	18.2	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), α-ethyl-L-glutamate
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	α-ethyl-L-glutamate10 mM MnCl2	—	0.53	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), α-ethyl-L-glutamate
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	α-methyl-L-glutamate25 mM MgCl2	—	3.9	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), α-methyl-L-glutamate
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	α-methyl-L-glutamate10 mM MnCl2	—	0.41	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), α-methyl-L-glutamate
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	β-amino-iso-butyrate25 mM MgCl2	—	29.9	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), β-amino-iso-butyrate
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	β-amino-iso-butyrate10 mM MnCl2	—	6.1	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), β-amino-iso-butyrate
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	β-chloro-L-alanine25 mM MgCl2	—	0.17	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), β-chloro-L-alanine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	β-chloro-L-alanine10 mM MnCl2	—	0.031	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), β-chloro-L-alanine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	β-cyano-L-alanine25 mM MgCl2	—	0.28	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), β-cyano-L-alanine
γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase)	Escherichia coli	β-cyano-L-alanine10 mM MnCl2	—	0.028	—	pH 8.2, 37 °C	PMID 11675389	Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), β-cyano-L-alanine