Values CatLog read from this paper
Redox-dependent stability of the γ-glutamylcysteine synthetase enzyme of Escherichia coli: a novel means of redox regulation
PMID 23126248doi:10.1042/bj20120204Open in Browse
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CatLog read 21 records (63 values) from this paper: γ-glutamylcysteine synthetase (γ-GCS) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| γ-glutamylcysteine synthetase (γ-GCS) | Escherichia coli | adenosine 5'-triphosphateTable 2 | 46.9 | 0.14 | 335 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS), adenosine 5'-triphosphate |
| γ-glutamylcysteine synthetase (γ-GCS) | Escherichia coli | adenosine 5'-triphosphateTable 3 | 28.7 | 0.46 | 62.4 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS), adenosine 5'-triphosphate |
| γ-glutamylcysteine synthetase (γ-GCS) | Escherichia coli | L-cysteineTable 2 | 46.6 | 0.1 | 466 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS), L-cysteine |
| γ-glutamylcysteine synthetase (γ-GCS) | Escherichia coli | L-cysteineTable 3 | 24.2 | 0.14 | 172.9 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS), L-cysteine |
| γ-glutamylcysteine synthetase (γ-GCS) | Escherichia coli | L-glutamic acidTable 2 | 31.4 | 3.9 | 8.1 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS), L-glutamic acid |
| γ-glutamylcysteine synthetase (γ-GCS) | Escherichia coli | L-glutamic acidTable 3 | 22.1 | 5.1 | 4.3 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS), L-glutamic acid |
| γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C372S/R374Q/V375F/C395S/C433S/C439S | Escherichia coli | adenosine 5'-triphosphate | 27.2 | 0.19 | 142.9 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C372S/R374Q/V375F/C395S/C433S/C439S, adenosine 5'-triphosphate |
| γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C372S/R374Q/V375F/C395S/C433S/C439S | Escherichia coli | L-cysteine | 29.2 | 0.14 | 208.6 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C372S/R374Q/V375F/C395S/C433S/C439S, L-cysteine |
| γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C372S/R374Q/V375F/C395S/C433S/C439S | Escherichia coli | L-glutamic acid | 29.6 | 4 | 7.4 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C372S/R374Q/V375F/C395S/C433S/C439S, L-glutamic acid |
| γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C395W/C433S/C439S | Escherichia coli | adenosine 5'-triphosphate | 30.5 | 0.24 | 127.1 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C395W/C433S/C439S, adenosine 5'-triphosphate |
| γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C395W/C433S/C439S | Escherichia coli | L-cysteine | 31.8 | 0.16 | 198.8 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C395W/C433S/C439S, L-cysteine |
| γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C395W/C433S/C439S | Escherichia coli | L-glutamic acid | 30.4 | 4.1 | 7.4 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C395W/C433S/C439S, L-glutamic acid |
| γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C395Y/C433S/C439S | Escherichia coli | adenosine 5'-triphosphate | 24.7 | 0.53 | 46.6 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C395Y/C433S/C439S, adenosine 5'-triphosphate |
| γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C395Y/C433S/C439S | Escherichia coli | L-cysteine | 21.9 | 0.11 | 199.1 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C395Y/C433S/C439S, L-cysteine |
| γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C395Y/C433S/C439S | Escherichia coli | L-glutamic acid | 22.1 | 3.2 | 6.9 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C395Y/C433S/C439S, L-glutamic acid |
| γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C433S/C439S | Escherichia coli | adenosine 5'-triphosphateTable 2 | 39.7 | 0.1 | 397 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C433S/C439S, adenosine 5'-triphosphate |
| γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C433S/C439S | Escherichia coli | adenosine 5'-triphosphateTable 3 | 30.1 | 0.25 | 120.4 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C433S/C439S, adenosine 5'-triphosphate |
| γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C433S/C439S | Escherichia coli | L-cysteineTable 2 | 39.7 | 0.11 | 396.9 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C433S/C439S, L-cysteine |
| γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C433S/C439S | Escherichia coli | L-cysteineTable 3 | 22.2 | 0.1 | 222 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C433S/C439S, L-cysteine |
| γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C433S/C439S | Escherichia coli | L-glutamic acidTable 2 | 34 | 4 | 8.5 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C433S/C439S, L-glutamic acid |
| γ-glutamylcysteine synthetase (γ-GCS)variant C106S/C164S/C205S/C223S/C357S/C433S/C439S | Escherichia coli | L-glutamic acidTable 3 | 23.8 | 5.3 | 4.5 | pH 8, 25 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS) variant C106S/C164S/C205S/C223S/C357S/C433S/C439S, L-glutamic acid |