Values CatLog read from this paper

Substrate binding determinants of Trypanosoma brucei gamma-glutamylcysteine synthetase

PMID 11851421doi:10.1021/bi0159128Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 20 records (28 values) from this paper: γ-glutamylcysteine synthetase from Trypanosoma brucei.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.3.2.2

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
γ-glutamylcysteine synthetase	Trypanosoma brucei	gamma-aminobutyric acid	0.14	150	9.3 × 10⁻⁴ calc.	37 °C	Open record: γ-glutamylcysteine synthetase, gamma-aminobutyric acid
γ-glutamylcysteine synthetasevariant R179A	Trypanosoma brucei	ATP	—	0.14	—	37 °C	Open record: γ-glutamylcysteine synthetase variant R179A, ATP
γ-glutamylcysteine synthetasevariant R179A	Trypanosoma brucei	L-alpha-aminobutyrate	—	380	—	37 °C	Open record: γ-glutamylcysteine synthetase variant R179A, L-alpha-aminobutyrate
γ-glutamylcysteine synthetasevariant R179A	Trypanosoma brucei	L-glutamate	0.61	5.2	0.12 calc.	37 °C	Open record: γ-glutamylcysteine synthetase variant R179A, L-glutamate
γ-glutamylcysteine synthetasevariant R366A	Trypanosoma brucei	ATP	—	2.8	—	37 °C	Open record: γ-glutamylcysteine synthetase variant R366A, ATP
γ-glutamylcysteine synthetasevariant R366A	Trypanosoma brucei	gamma-aminobutyric acid	0.59	20	0.029 calc.	37 °C	Open record: γ-glutamylcysteine synthetase variant R366A, gamma-aminobutyric acid
γ-glutamylcysteine synthetasevariant R366A	Trypanosoma brucei	L-alpha-aminobutyrate	—	34	—	37 °C	Open record: γ-glutamylcysteine synthetase variant R366A, L-alpha-aminobutyrate
γ-glutamylcysteine synthetasevariant R366A	Trypanosoma brucei	L-glutamate	0.32	130	0.0025 calc.	37 °C	Open record: γ-glutamylcysteine synthetase variant R366A, L-glutamate
γ-glutamylcysteine synthetasevariant R474A	Trypanosoma brucei	ATP	—	1.3	—	37 °C	Open record: γ-glutamylcysteine synthetase variant R474A, ATP
γ-glutamylcysteine synthetasevariant R474A	Trypanosoma brucei	L-alpha-aminobutyrate	—	56	—	37 °C	Open record: γ-glutamylcysteine synthetase variant R474A, L-alpha-aminobutyrate
γ-glutamylcysteine synthetasevariant R474A	Trypanosoma brucei	L-glutamate	0.24	1.6	0.15 calc.	37 °C	Open record: γ-glutamylcysteine synthetase variant R474A, L-glutamate
γ-glutamylcysteine synthetasevariant R487A	Trypanosoma brucei	ATP	—	1.2	—	37 °C	Open record: γ-glutamylcysteine synthetase variant R487A, ATP
γ-glutamylcysteine synthetasevariant R487A	Trypanosoma brucei	L-alpha-aminobutyrate	—	15	—	37 °C	Open record: γ-glutamylcysteine synthetase variant R487A, L-alpha-aminobutyrate
γ-glutamylcysteine synthetasevariant R487A	Trypanosoma brucei	L-glutamate	1.2	1.3	0.92 calc.	37 °C	Open record: γ-glutamylcysteine synthetase variant R487A, L-glutamate
γ-glutamylcysteine synthetasevariant R491A	Trypanosoma brucei	ATP	—	0.11	—	37 °C	Open record: γ-glutamylcysteine synthetase variant R491A, ATP
γ-glutamylcysteine synthetasevariant R491A	Trypanosoma brucei	L-glutamate	0.055	0.61	0.090 calc.	37 °C	Open record: γ-glutamylcysteine synthetase variant R491A, L-glutamate
γ-glutamylcysteine synthetasevariant T323A	Trypanosoma brucei	ATP	—	7	—	37 °C	Open record: γ-glutamylcysteine synthetase variant T323A, ATP
γ-glutamylcysteine synthetasevariant T323A	Trypanosoma brucei	L-alpha-aminobutyrate	—	7.5	—	37 °C	Open record: γ-glutamylcysteine synthetase variant T323A, L-alpha-aminobutyrate
γ-glutamylcysteine synthetasevariant T323A	Trypanosoma brucei	L-glutamate20 mM total MgCl2	0.79	1.7	0.46 calc.	37 °C	Open record: γ-glutamylcysteine synthetase variant T323A, L-glutamate
γ-glutamylcysteine synthetasevariant T323A	Trypanosoma brucei	L-glutamateMnCl2 instead of MgCl2; substrates at the standard 5 mM ATP	6	—	—	37 °C	Open record: γ-glutamylcysteine synthetase variant T323A, L-glutamate