Values CatLog read from this paper
Characterization of the bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase (GshF) of Pasteurella multocida
PMID 16339152doi:10.1074/jbc.m509517200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 7 records (19 values) from this paper: GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase) from Pasteurella multocida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase) | Pasteurella multocida | adenosine 5'-triphosphate | 22.4 | 0.25 | 89.6 | pH 8.2, 25 °C | Open record: GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase), adenosine 5'-triphosphate |
| GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase) | Pasteurella multocida | adenosine 5'-triphosphate | 26.5 | 0.53 | 50 | pH 8.2, 25 °C | Open record: GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase), adenosine 5'-triphosphate |
| GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase) | Pasteurella multocida | gamma-L-glutamyl-L-cysteine | 26.9 | 0.09 | 274 | pH 8.2, 25 °C | Open record: GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase), gamma-L-glutamyl-L-cysteine |
| GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase) | Pasteurella multocida | glycineADP formation by coupled assay: PK/LDH with 0.25 mM NADH | 36.3 | 81 | 0.448 | pH 8.2, 25 °C | Open record: GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase), glycine |
| GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase) | Pasteurella multocida | glycine50 mM L-Glu; 2 mM L-Cys; 5 mM ATP | — | 1.72 | — | pH 8.2, 25 °C | Open record: GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase), glycine |
| GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase) | Pasteurella multocida | L-cysteine | 25.3 | 0.22 | 115 | pH 8.2, 25 °C | Open record: GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase), L-cysteine |
| GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase) | Pasteurella multocida | L-glutamate | 24.3 | 5.3 | 4.58 | pH 8.2, 25 °C | Open record: GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase), L-glutamate |