EC 6.3.2.3: glutathione synthase

LigasesOpen in Browse

CatLog read 121 records for EC 6.3.2.3 from 8 papers, for 6 organisms. Names in these papers: glutathione synthetase (AtGS), glutathione synthetase, mxGs (glutathione synthetase), glutathione synthetase (rPhGshB) and 2 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 98 records for EC 6.3.2.3 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.3.2.3 in Browse.

Papers

Papers with records for EC 6.3.2.3
Paper	Organisms	Records
Reaction mechanism of glutathione synthetase from Arabidopsis thaliana: site-directed mutagenesis of active site residues	Arabidopsis thaliana	50
Human hereditary glutathione synthetase deficiency: kinetic properties of mutant enzymes	Homo sapiens	16
Function of conserved residues of human glutathione synthetase: implications for the ATP-grasp enzymes	Homo sapiens	15
Aspartate 458 of human glutathione synthetase is important for cooperativity and active site structure	Homo sapiens	12
Characterization of glutamate-cysteine ligase and glutathione synthetase from the δ-proteobacterium Myxococcus xanthus	Myxococcus xanthus	12
Characterization of a cold-adapted glutathione synthetase from the psychrophile Pseudoalteromonas haloplanktis	Pseudoalteromonas haloplanktis	8
Characterization of the bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase (GshF) of Pasteurella multocida	Pasteurella multocida	4
Probing the origins of glutathione biosynthesis through biochemical analysis of glutamate-cysteine ligase and glutathione synthetase from a model photosynthetic prokaryote	Synechocystis sp. PCC 6803	4

Records

Records for EC 6.3.2.3 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
glutathione synthetase	Homo sapiens	ATP	—	0.07	—	pH 8.2, 37 °C	PMID 14990577	Open record: glutathione synthetase, ATP
glutathione synthetase	Homo sapiens	ATP	—	0.07	—	pH 8.2, 37 °C	PMID 15056072	Open record: glutathione synthetase, ATP
glutathione synthetase	Homo sapiens	glycine	—	1.75	—	pH 8.2, 37 °C	PMID 14990577	Open record: glutathione synthetase, glycine
glutathione synthetase	Homo sapiens	glycine	—	1.75	—	pH 8.2, 37 °C	PMID 15056072	Open record: glutathione synthetase, glycine
glutathione synthetase	Homo sapiens	glycine	—	0.58	—	pH 8.2, 37 °C	PMID 21771585	Open record: glutathione synthetase, glycine
glutathione synthetase	Homo sapiens	γ-glutamyl-α-aminobutyrate	6.5	—	—	pH 8.2, 37 °C	PMID 14990577	Open record: glutathione synthetase, γ-glutamyl-α-aminobutyrate
glutathione synthetase	Homo sapiens	γ-glutamyl-α-aminobutyrate	15.68	—	—	pH 8.2, 37 °C	PMID 21771585	Open record: glutathione synthetase, γ-glutamyl-α-aminobutyrate
glutathione synthetasevariant D219A	Homo sapiens	ATP	—	1.05	—	pH 8.2, 37 °C	PMID 15056072	Open record: glutathione synthetase variant D219A, ATP
glutathione synthetasevariant D219A	Homo sapiens	glycine	—	2.07	—	pH 8.2, 37 °C	PMID 15056072	Open record: glutathione synthetase variant D219A, glycine
glutathione synthetasevariant D219G	Homo sapiens	ATP	—	0.04	—	pH 8.2, 37 °C	PMID 15056072	Open record: glutathione synthetase variant D219G, ATP
glutathione synthetasevariant D219G	Homo sapiens	glycine	—	1.51	—	pH 8.2, 37 °C	PMID 15056072	Open record: glutathione synthetase variant D219G, glycine
glutathione synthetasevariant D458A	Homo sapiens	glycine	—	35	—	pH 8.2, 37 °C	PMID 21771585	Open record: glutathione synthetase variant D458A, glycine
glutathione synthetasevariant D458A	Homo sapiens	γ-glutamyl-α-aminobutyratestandard assay: 20 mM GAB; 10 mM glycine; 10 mM ATP	1.6	—	—	pH 8.2, 37 °C	PMID 21771585	Open record: glutathione synthetase variant D458A, γ-glutamyl-α-aminobutyrate
glutathione synthetasevariant D458A	Homo sapiens	γ-glutamyl-α-aminobutyrateother substrates saturating	—	0.25	—	pH 8.2, 37 °C	PMID 21771585	Open record: glutathione synthetase variant D458A, γ-glutamyl-α-aminobutyrate
glutathione synthetasevariant D458N	Homo sapiens	glycine	—	16	—	pH 8.2, 37 °C	PMID 21771585	Open record: glutathione synthetase variant D458N, glycine
glutathione synthetasevariant D458N	Homo sapiens	glycineenzyme re-assayed after storage; at peak activity	—	0.77	—	pH 8.2, 37 °C	PMID 21771585	Open record: glutathione synthetase variant D458N, glycine
glutathione synthetasevariant D458N	Homo sapiens	γ-glutamyl-α-aminobutyratestandard assay: 20 mM GAB; 10 mM glycine; 10 mM ATP	2.31	—	—	pH 8.2, 37 °C	PMID 21771585	Open record: glutathione synthetase variant D458N, γ-glutamyl-α-aminobutyrate
glutathione synthetasevariant D458N	Homo sapiens	γ-glutamyl-α-aminobutyrateother substrates saturating	—	0.12	—	pH 8.2, 37 °C	PMID 21771585	Open record: glutathione synthetase variant D458N, γ-glutamyl-α-aminobutyrate
glutathione synthetasevariant D458R	Homo sapiens	glycine	—	51	—	pH 8.2, 37 °C	PMID 21771585	Open record: glutathione synthetase variant D458R, glycine
glutathione synthetasevariant D458R	Homo sapiens	γ-glutamyl-α-aminobutyratestandard assay: 20 mM GAB; 10 mM glycine; 10 mM ATP	1.17	—	—	pH 8.2, 37 °C	PMID 21771585	Open record: glutathione synthetase variant D458R, γ-glutamyl-α-aminobutyrate
glutathione synthetasevariant D458R	Homo sapiens	γ-glutamyl-α-aminobutyrateother substrates saturating	—	0.29	—	pH 8.2, 37 °C	PMID 21771585	Open record: glutathione synthetase variant D458R, γ-glutamyl-α-aminobutyrate
glutathione synthetasevariant E144A	Homo sapiens	γ-glutamyl-α-aminobutyrate	0.003	—	—	pH 8.2, 37 °C	PMID 14990577	Open record: glutathione synthetase variant E144A, γ-glutamyl-α-aminobutyrate
glutathione synthetasevariant K305A	Homo sapiens	ATP	—	0.83	—	pH 8.2, 37 °C	PMID 14990577	Open record: glutathione synthetase variant K305A, ATP
glutathione synthetasevariant K305A	Homo sapiens	glycine	—	0.25	—	pH 8.2, 37 °C	PMID 14990577	Open record: glutathione synthetase variant K305A, glycine
glutathione synthetasevariant K305A	Homo sapiens	γ-glutamyl-α-aminobutyrate	0.423	—	—	pH 8.2, 37 °C	PMID 14990577	Open record: glutathione synthetase variant K305A, γ-glutamyl-α-aminobutyrate
glutathione synthetasevariant K305A	Homo sapiens	γ-glutamyl-α-aminobutyrateapparent Km	—	0.34	—	pH 8.2, 37 °C	PMID 14990577	Open record: glutathione synthetase variant K305A, γ-glutamyl-α-aminobutyrate
glutathione synthetasevariant K305E	Homo sapiens	ATP	—	2.66	—	pH 8.2, 37 °C	PMID 14990577	Open record: glutathione synthetase variant K305E, ATP
glutathione synthetasevariant K305E	Homo sapiens	glycine	—	1.96	—	pH 8.2, 37 °C	PMID 14990577	Open record: glutathione synthetase variant K305E, glycine
glutathione synthetasevariant K305E	Homo sapiens	γ-glutamyl-α-aminobutyrate	0.336	—	—	pH 8.2, 37 °C	PMID 14990577	Open record: glutathione synthetase variant K305E, γ-glutamyl-α-aminobutyrate
glutathione synthetasevariant K364A	Homo sapiens	γ-glutamyl-α-aminobutyrate	0.008	—	—	pH 8.2, 37 °C	PMID 14990577	Open record: glutathione synthetase variant K364A, γ-glutamyl-α-aminobutyrate
glutathione synthetasevariant K364E	Homo sapiens	γ-glutamyl-α-aminobutyrate	0.015	—	—	pH 8.2, 37 °C	PMID 14990577	Open record: glutathione synthetase variant K364E, γ-glutamyl-α-aminobutyrate
glutathione synthetasevariant L188P	Homo sapiens	ATP	—	0.73	—	pH 8.2, 37 °C	PMID 15056072	Open record: glutathione synthetase variant L188P, ATP
glutathione synthetasevariant L188P	Homo sapiens	glycine	—	2.25	—	pH 8.2, 37 °C	PMID 15056072	Open record: glutathione synthetase variant L188P, glycine
glutathione synthetasevariant N146A	Homo sapiens	γ-glutamyl-α-aminobutyrate	0.007	—	—	pH 8.2, 37 °C	PMID 14990577	Open record: glutathione synthetase variant N146A, γ-glutamyl-α-aminobutyrate
glutathione synthetasevariant N146D	Homo sapiens	γ-glutamyl-α-aminobutyrate	0.003	—	—	pH 8.2, 37 °C	PMID 14990577	Open record: glutathione synthetase variant N146D, γ-glutamyl-α-aminobutyrate
glutathione synthetasevariant P314L	Homo sapiens	ATP	—	0.05	—	pH 8.2, 37 °C	PMID 15056072	Open record: glutathione synthetase variant P314L, ATP
glutathione synthetasevariant P314L	Homo sapiens	glycine	—	1.64	—	pH 8.2, 37 °C	PMID 15056072	Open record: glutathione synthetase variant P314L, glycine
glutathione synthetasevariant R283C	Homo sapiens	ATP	—	0.09	—	pH 8.2, 37 °C	PMID 15056072	Open record: glutathione synthetase variant R283C, ATP
glutathione synthetasevariant R283C	Homo sapiens	glycine	—	1.02	—	pH 8.2, 37 °C	PMID 15056072	Open record: glutathione synthetase variant R283C, glycine
glutathione synthetasevariant Y270C	Homo sapiens	ATP	—	0.05	—	pH 8.2, 37 °C	PMID 15056072	Open record: glutathione synthetase variant Y270C, ATP
glutathione synthetasevariant Y270C	Homo sapiens	glycine	—	1.43	—	pH 8.2, 37 °C	PMID 15056072	Open record: glutathione synthetase variant Y270C, glycine
glutathione synthetasevariant Y270H	Homo sapiens	ATP	—	0.02	—	pH 8.2, 37 °C	PMID 15056072	Open record: glutathione synthetase variant Y270H, ATP
glutathione synthetasevariant Y270H	Homo sapiens	glycine	—	1.24	—	pH 8.2, 37 °C	PMID 15056072	Open record: glutathione synthetase variant Y270H, glycine
glutathione synthetase (AtGS)	Arabidopsis thaliana	ATP	50.4	—	465	25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS), ATP
glutathione synthetase (AtGS)	Arabidopsis thaliana	glycine	48.8	—	244	25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS), glycine
glutathione synthetase (AtGS)	Arabidopsis thaliana	γ-glutamylcysteine	53.9	—	495	25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS), γ-glutamylcysteine
glutathione synthetase (AtGS)variant E220A	Arabidopsis thaliana	ATP	1.46	0.18	8.11	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant E220A, ATP
glutathione synthetase (AtGS)variant E220A	Arabidopsis thaliana	glycine	1.08	4.04	0.267	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant E220A, glycine
glutathione synthetase (AtGS)variant E220A	Arabidopsis thaliana	γ-glutamylcysteine	1.51	1.94	0.778	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant E220A, γ-glutamylcysteine
glutathione synthetase (AtGS)variant E220Q	Arabidopsis thaliana	ATP	3.18	0.19	16.8	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant E220Q, ATP
glutathione synthetase (AtGS)variant E220Q	Arabidopsis thaliana	glycine	2.58	1.67	1.55	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant E220Q, glycine
glutathione synthetase (AtGS)variant E220Q	Arabidopsis thaliana	γ-glutamylcysteine	3.25	0.954	3.41	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant E220Q, γ-glutamylcysteine
glutathione synthetase (AtGS)variant E371Q	Arabidopsis thaliana	ATP	0.17	0.346	0.491	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant E371Q, ATP
glutathione synthetase (AtGS)variant E371Q	Arabidopsis thaliana	glycine	0.1572	1.18	0.133	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant E371Q, glycine
glutathione synthetase (AtGS)variant E371Q	Arabidopsis thaliana	γ-glutamylcysteine	0.1582	0.273	0.579	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant E371Q, γ-glutamylcysteine
glutathione synthetase (AtGS)variant K456M	Arabidopsis thaliana	ATP	0.15	0.695	0.216	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant K456M, ATP
glutathione synthetase (AtGS)variant K456M	Arabidopsis thaliana	glycine	0.29	2.4	0.121	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant K456M, glycine
glutathione synthetase (AtGS)variant K456M	Arabidopsis thaliana	γ-glutamylcysteine	0.109	0.425	0.256	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant K456M, γ-glutamylcysteine
glutathione synthetase (AtGS)variant N376A	Arabidopsis thaliana	ATP	0.61	0.785	0.777	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant N376A, ATP
glutathione synthetase (AtGS)variant N376A	Arabidopsis thaliana	glycine	1.03	6.59	0.156	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant N376A, glycine
glutathione synthetase (AtGS)variant N376A	Arabidopsis thaliana	γ-glutamylcysteine	0.383	0.271	1.413	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant N376A, γ-glutamylcysteine
glutathione synthetase (AtGS)variant Q226A	Arabidopsis thaliana	ATP	0.72	0.089	8.09	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant Q226A, ATP
glutathione synthetase (AtGS)variant Q226A	Arabidopsis thaliana	glycine	0.37	3.45	0.107	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant Q226A, glycine
glutathione synthetase (AtGS)variant Q226A	Arabidopsis thaliana	γ-glutamylcysteine	0.973	3.6	0.27	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant Q226A, γ-glutamylcysteine
glutathione synthetase (AtGS)variant Q226N	Arabidopsis thaliana	ATP	1.593	0.194	8.21	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant Q226N, ATP
glutathione synthetase (AtGS)variant Q226N	Arabidopsis thaliana	glycine	1.33	1.69	0.79	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant Q226N, glycine
glutathione synthetase (AtGS)variant Q226N	Arabidopsis thaliana	γ-glutamylcysteine	1.485	3.34	0.445	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant Q226N, γ-glutamylcysteine
glutathione synthetase (AtGS)variant R132K	Arabidopsis thaliana	ATP	0.071	0.125	0.568	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R132K, ATP
glutathione synthetase (AtGS)variant R132K	Arabidopsis thaliana	ATP	—	—	0.5	25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R132K, ATP
glutathione synthetase (AtGS)variant R132K	Arabidopsis thaliana	glycine	0.062	7.57	0.00773	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R132K, glycine
glutathione synthetase (AtGS)variant R132K	Arabidopsis thaliana	glycine	—	—	0.00874	25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R132K, glycine
glutathione synthetase (AtGS)variant R132K	Arabidopsis thaliana	γ-glutamylcysteine	0.0585	0.129	0.454	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R132K, γ-glutamylcysteine
glutathione synthetase (AtGS)variant R132K	Arabidopsis thaliana	γ-glutamylcysteine	—	—	0.429	25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R132K, γ-glutamylcysteine
glutathione synthetase (AtGS)variant R274K	Arabidopsis thaliana	ATP	0.45	0.175	2.57	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R274K, ATP
glutathione synthetase (AtGS)variant R274K	Arabidopsis thaliana	glycine	0.22	2.16	0.102	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R274K, glycine
glutathione synthetase (AtGS)variant R274K	Arabidopsis thaliana	γ-glutamylcysteine	0.842	7.03	0.12	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R274K, γ-glutamylcysteine
glutathione synthetase (AtGS)variant R454A	Arabidopsis thaliana	ATP	0.0785	0.37	0.212	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R454A, ATP
glutathione synthetase (AtGS)variant R454A	Arabidopsis thaliana	ATP	—	—	0.33	25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R454A, ATP
glutathione synthetase (AtGS)variant R454A	Arabidopsis thaliana	glycine	0.0798	16.1	0.00462	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R454A, glycine
glutathione synthetase (AtGS)variant R454A	Arabidopsis thaliana	γ-glutamylcysteine	0.0773	0.146	0.53	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R454A, γ-glutamylcysteine
glutathione synthetase (AtGS)variant R454A	Arabidopsis thaliana	γ-glutamylcysteine	—	—	0.514	25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R454A, γ-glutamylcysteine
glutathione synthetase (AtGS)variant R454K	Arabidopsis thaliana	ATP	2.18	0.171	12.8	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R454K, ATP
glutathione synthetase (AtGS)variant R454K	Arabidopsis thaliana	ATP	—	—	19.01	25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R454K, ATP
glutathione synthetase (AtGS)variant R454K	Arabidopsis thaliana	glycine	2.47	4.17	0.592	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R454K, glycine
glutathione synthetase (AtGS)variant R454K	Arabidopsis thaliana	glycine	—	—	0.637	25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R454K, glycine
glutathione synthetase (AtGS)variant R454K	Arabidopsis thaliana	γ-glutamylcysteine	3.82	0.143	26.7	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R454K, γ-glutamylcysteine
glutathione synthetase (AtGS)variant R454K	Arabidopsis thaliana	γ-glutamylcysteine	—	—	20.42	25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant R454K, γ-glutamylcysteine
glutathione synthetase (AtGS)variant S153A	Arabidopsis thaliana	ATP	5.7	0.116	49.1	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant S153A, ATP
glutathione synthetase (AtGS)variant S153A	Arabidopsis thaliana	glycine	7.2	3.41	2.11	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant S153A, glycine
glutathione synthetase (AtGS)variant S153A	Arabidopsis thaliana	γ-glutamylcysteine	5.32	0.166	32.03	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant S153A, γ-glutamylcysteine
glutathione synthetase (AtGS)variant S155A	Arabidopsis thaliana	ATP	5.08	0.189	26.9	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant S155A, ATP
glutathione synthetase (AtGS)variant S155A	Arabidopsis thaliana	glycine	5.18	2.25	2.3	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant S155A, glycine
glutathione synthetase (AtGS)variant S155A	Arabidopsis thaliana	γ-glutamylcysteine	3.52	0.035	100.6	pH 7.5, 25 °C	PMID 17452339	Open record: glutathione synthetase (AtGS) variant S155A, γ-glutamylcysteine
glutathione synthetase (rPhGshB)	Pseudoalteromonas haloplanktis	adenosine 5'-triphosphate	0.87	0.17	5.1	pH 7.8, 10 °C	PMID 22777241	Open record: glutathione synthetase (rPhGshB), adenosine 5'-triphosphate
glutathione synthetase (rPhGshB)	Pseudoalteromonas haloplanktis	adenosine 5'-triphosphate	1.85	0.26	7.1	pH 7.8, 15 °C	PMID 22777241	Open record: glutathione synthetase (rPhGshB), adenosine 5'-triphosphate
glutathione synthetase (rPhGshB)	Pseudoalteromonas haloplanktis	adenosine 5'-triphosphate	2.42	0.18	13.4	pH 7.8, 20 °C	PMID 22777241	Open record: glutathione synthetase (rPhGshB), adenosine 5'-triphosphate
glutathione synthetase (rPhGshB)	Pseudoalteromonas haloplanktis	adenosine 5'-triphosphate	4.72	0.28	16.9	pH 7.8, 25 °C	PMID 22777241	Open record: glutathione synthetase (rPhGshB), adenosine 5'-triphosphate
glutathione synthetase (rPhGshB)	Pseudoalteromonas haloplanktis	adenosine 5'-triphosphate	4.99	0.21	23.8	pH 7.8, 28 °C	PMID 22777241	Open record: glutathione synthetase (rPhGshB), adenosine 5'-triphosphate
glutathione synthetase (rPhGshB)	Pseudoalteromonas haloplanktis	adenosine 5'-triphosphate	5.32	0.18	29.6	pH 7.8, 30 °C	PMID 22777241	Open record: glutathione synthetase (rPhGshB), adenosine 5'-triphosphate
glutathione synthetase (rPhGshB)	Pseudoalteromonas haloplanktis	gamma-L-glutamyl-L-cysteine	1.93	0.25	7.7 calc.	pH 7.8, 15 °C	PMID 22777241	Open record: glutathione synthetase (rPhGshB), gamma-L-glutamyl-L-cysteine
glutathione synthetase (rPhGshB)	Pseudoalteromonas haloplanktis	glycine	2.03	0.75	2.7 calc.	pH 7.8, 15 °C	PMID 22777241	Open record: glutathione synthetase (rPhGshB), glycine
glutathione synthetase (SynGS)	Synechocystis sp. PCC 6803	ATP	5.4	0.136	39.71	pH 7.5, 25 °C	PMID 23170977	Open record: glutathione synthetase (SynGS), ATP
glutathione synthetase (SynGS)	Synechocystis sp. PCC 6803	Glycine	4.22	0.407	10.36	pH 7.5, 25 °C	PMID 23170977	Open record: glutathione synthetase (SynGS), Glycine
glutathione synthetase (SynGS)	Synechocystis sp. PCC 6803	γ-Glutamylcysteinevaried substrate with the other two fixed at saturation	4.62	0.099	46.63	pH 7.5, 25 °C	PMID 23170977	Open record: glutathione synthetase (SynGS), γ-Glutamylcysteine
glutathione synthetase (SynGS)	Synechocystis sp. PCC 6803	γ-Glutamylcysteinematrix of γ-glutamylcysteine 0.05-0.5 mM; ATP 0.1-1.2 mM	3.07	—	—	pH 7.5, 25 °C	PMID 23170977	Open record: glutathione synthetase (SynGS), γ-Glutamylcysteine
GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase)	Pasteurella multocida	adenosine 5'-triphosphate	26.5	0.53	50	pH 8.2, 25 °C	PMID 16339152	Open record: GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase), adenosine 5'-triphosphate
GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase)	Pasteurella multocida	gamma-L-glutamyl-L-cysteine	26.9	0.09	274	pH 8.2, 25 °C	PMID 16339152	Open record: GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase), gamma-L-glutamyl-L-cysteine
GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase)	Pasteurella multocida	glycineADP formation by coupled assay: PK/LDH with 0.25 mM NADH	36.3	81	0.448	pH 8.2, 25 °C	PMID 16339152	Open record: GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase), glycine
GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase)	Pasteurella multocida	glycine50 mM L-Glu; 2 mM L-Cys; 5 mM ATP	—	1.72	—	pH 8.2, 25 °C	PMID 16339152	Open record: GshF (bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase), glycine
mxGs (glutathione synthetase)	Myxococcus xanthus	adenosine 5'-triphosphate	3.77	1.22	3.09	pH 9, 37 °C	PMID 35277888	Open record: mxGs (glutathione synthetase), adenosine 5'-triphosphate
mxGs (glutathione synthetase)	Myxococcus xanthus	glycine	3.04	0.24	12.7	pH 9, 37 °C	PMID 35277888	Open record: mxGs (glutathione synthetase), glycine
mxGs (glutathione synthetase)	Myxococcus xanthus	γ-glutamylcysteine	3.96	1.2	3.3	pH 9, 37 °C	PMID 35277888	Open record: mxGs (glutathione synthetase), γ-glutamylcysteine
mxGs (glutathione synthetase)variant A170G	Myxococcus xanthus	adenosine 5'-triphosphate	5.36	1.6	3.35	pH 9, 37 °C	PMID 35277888	Open record: mxGs (glutathione synthetase) variant A170G, adenosine 5'-triphosphate
mxGs (glutathione synthetase)variant A170G	Myxococcus xanthus	glycine	5.22	0.48	10.9	pH 9, 37 °C	PMID 35277888	Open record: mxGs (glutathione synthetase) variant A170G, glycine
mxGs (glutathione synthetase)variant A170G	Myxococcus xanthus	γ-glutamylcysteine	6.52	1.9	3.43	pH 9, 37 °C	PMID 35277888	Open record: mxGs (glutathione synthetase) variant A170G, γ-glutamylcysteine
mxGs (glutathione synthetase)variant M239L	Myxococcus xanthus	adenosine 5'-triphosphate	2.31	1.7	1.36	pH 9, 37 °C	PMID 35277888	Open record: mxGs (glutathione synthetase) variant M239L, adenosine 5'-triphosphate
mxGs (glutathione synthetase)variant M239L	Myxococcus xanthus	glycine	2.41	0.38	6.34	pH 9, 37 °C	PMID 35277888	Open record: mxGs (glutathione synthetase) variant M239L, glycine
mxGs (glutathione synthetase)variant M239L	Myxococcus xanthus	γ-glutamylcysteine	3.39	1.75	1.94	pH 9, 37 °C	PMID 35277888	Open record: mxGs (glutathione synthetase) variant M239L, γ-glutamylcysteine
mxGs (glutathione synthetase)variant T23S	Myxococcus xanthus	adenosine 5'-triphosphate	2.18	2.13	1.02	pH 9, 37 °C	PMID 35277888	Open record: mxGs (glutathione synthetase) variant T23S, adenosine 5'-triphosphate
mxGs (glutathione synthetase)variant T23S	Myxococcus xanthus	glycine	2.96	0.5	5.92	pH 9, 37 °C	PMID 35277888	Open record: mxGs (glutathione synthetase) variant T23S, glycine
mxGs (glutathione synthetase)variant T23S	Myxococcus xanthus	γ-glutamylcysteine	4.02	1.93	2.08	pH 9, 37 °C	PMID 35277888	Open record: mxGs (glutathione synthetase) variant T23S, γ-glutamylcysteine