Values CatLog read from this paper
Reaction mechanism of glutathione synthetase from Arabidopsis thaliana: site-directed mutagenesis of active site residues
PMID 17452339doi:10.1074/jbc.m700804200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 50 records (131 values) from this paper: glutathione synthetase (AtGS) from Arabidopsis thaliana.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glutathione synthetase (AtGS) | Arabidopsis thaliana | ATP | 50.4 | — | 465 | 25 °C | Open record: glutathione synthetase (AtGS), ATP |
| glutathione synthetase (AtGS) | Arabidopsis thaliana | glycine | 48.8 | — | 244 | 25 °C | Open record: glutathione synthetase (AtGS), glycine |
| glutathione synthetase (AtGS) | Arabidopsis thaliana | γ-glutamylcysteine | 53.9 | — | 495 | 25 °C | Open record: glutathione synthetase (AtGS), γ-glutamylcysteine |
| glutathione synthetase (AtGS)variant E220A | Arabidopsis thaliana | ATP | 1.46 | 0.18 | 8.11 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant E220A, ATP |
| glutathione synthetase (AtGS)variant E220A | Arabidopsis thaliana | glycine | 1.08 | 4.04 | 0.267 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant E220A, glycine |
| glutathione synthetase (AtGS)variant E220A | Arabidopsis thaliana | γ-glutamylcysteine | 1.51 | 1.94 | 0.778 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant E220A, γ-glutamylcysteine |
| glutathione synthetase (AtGS)variant E220Q | Arabidopsis thaliana | ATP | 3.18 | 0.19 | 16.8 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant E220Q, ATP |
| glutathione synthetase (AtGS)variant E220Q | Arabidopsis thaliana | glycine | 2.58 | 1.67 | 1.55 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant E220Q, glycine |
| glutathione synthetase (AtGS)variant E220Q | Arabidopsis thaliana | γ-glutamylcysteine | 3.25 | 0.954 | 3.41 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant E220Q, γ-glutamylcysteine |
| glutathione synthetase (AtGS)variant E371Q | Arabidopsis thaliana | ATP | 0.17 | 0.346 | 0.491 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant E371Q, ATP |
| glutathione synthetase (AtGS)variant E371Q | Arabidopsis thaliana | glycine | 0.1572 | 1.18 | 0.133 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant E371Q, glycine |
| glutathione synthetase (AtGS)variant E371Q | Arabidopsis thaliana | γ-glutamylcysteine | 0.1582 | 0.273 | 0.579 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant E371Q, γ-glutamylcysteine |
| glutathione synthetase (AtGS)variant K456M | Arabidopsis thaliana | ATP | 0.15 | 0.695 | 0.216 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant K456M, ATP |
| glutathione synthetase (AtGS)variant K456M | Arabidopsis thaliana | glycine | 0.29 | 2.4 | 0.121 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant K456M, glycine |
| glutathione synthetase (AtGS)variant K456M | Arabidopsis thaliana | γ-glutamylcysteine | 0.109 | 0.425 | 0.256 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant K456M, γ-glutamylcysteine |
| glutathione synthetase (AtGS)variant N376A | Arabidopsis thaliana | ATP | 0.61 | 0.785 | 0.777 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant N376A, ATP |
| glutathione synthetase (AtGS)variant N376A | Arabidopsis thaliana | glycine | 1.03 | 6.59 | 0.156 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant N376A, glycine |
| glutathione synthetase (AtGS)variant N376A | Arabidopsis thaliana | γ-glutamylcysteine | 0.383 | 0.271 | 1.413 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant N376A, γ-glutamylcysteine |
| glutathione synthetase (AtGS)variant Q226A | Arabidopsis thaliana | ATP | 0.72 | 0.089 | 8.09 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant Q226A, ATP |
| glutathione synthetase (AtGS)variant Q226A | Arabidopsis thaliana | glycine | 0.37 | 3.45 | 0.107 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant Q226A, glycine |
| glutathione synthetase (AtGS)variant Q226A | Arabidopsis thaliana | γ-glutamylcysteine | 0.973 | 3.6 | 0.27 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant Q226A, γ-glutamylcysteine |
| glutathione synthetase (AtGS)variant Q226N | Arabidopsis thaliana | ATP | 1.593 | 0.194 | 8.21 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant Q226N, ATP |
| glutathione synthetase (AtGS)variant Q226N | Arabidopsis thaliana | glycine | 1.33 | 1.69 | 0.79 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant Q226N, glycine |
| glutathione synthetase (AtGS)variant Q226N | Arabidopsis thaliana | γ-glutamylcysteine | 1.485 | 3.34 | 0.445 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant Q226N, γ-glutamylcysteine |
| glutathione synthetase (AtGS)variant R132K | Arabidopsis thaliana | ATP | 0.071 | 0.125 | 0.568 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant R132K, ATP |
| glutathione synthetase (AtGS)variant R132K | Arabidopsis thaliana | ATP | — | — | 0.5 | 25 °C | Open record: glutathione synthetase (AtGS) variant R132K, ATP |
| glutathione synthetase (AtGS)variant R132K | Arabidopsis thaliana | glycine | 0.062 | 7.57 | 0.00773 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant R132K, glycine |
| glutathione synthetase (AtGS)variant R132K | Arabidopsis thaliana | glycine | — | — | 0.00874 | 25 °C | Open record: glutathione synthetase (AtGS) variant R132K, glycine |
| glutathione synthetase (AtGS)variant R132K | Arabidopsis thaliana | γ-glutamylcysteine | 0.0585 | 0.129 | 0.454 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant R132K, γ-glutamylcysteine |
| glutathione synthetase (AtGS)variant R132K | Arabidopsis thaliana | γ-glutamylcysteine | — | — | 0.429 | 25 °C | Open record: glutathione synthetase (AtGS) variant R132K, γ-glutamylcysteine |
| glutathione synthetase (AtGS)variant R274K | Arabidopsis thaliana | ATP | 0.45 | 0.175 | 2.57 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant R274K, ATP |
| glutathione synthetase (AtGS)variant R274K | Arabidopsis thaliana | glycine | 0.22 | 2.16 | 0.102 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant R274K, glycine |
| glutathione synthetase (AtGS)variant R274K | Arabidopsis thaliana | γ-glutamylcysteine | 0.842 | 7.03 | 0.12 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant R274K, γ-glutamylcysteine |
| glutathione synthetase (AtGS)variant R454A | Arabidopsis thaliana | ATP | 0.0785 | 0.37 | 0.212 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant R454A, ATP |
| glutathione synthetase (AtGS)variant R454A | Arabidopsis thaliana | ATP | — | — | 0.33 | 25 °C | Open record: glutathione synthetase (AtGS) variant R454A, ATP |
| glutathione synthetase (AtGS)variant R454A | Arabidopsis thaliana | glycine | 0.0798 | 16.1 | 0.00462 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant R454A, glycine |
| glutathione synthetase (AtGS)variant R454A | Arabidopsis thaliana | γ-glutamylcysteine | 0.0773 | 0.146 | 0.53 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant R454A, γ-glutamylcysteine |
| glutathione synthetase (AtGS)variant R454A | Arabidopsis thaliana | γ-glutamylcysteine | — | — | 0.514 | 25 °C | Open record: glutathione synthetase (AtGS) variant R454A, γ-glutamylcysteine |
| glutathione synthetase (AtGS)variant R454K | Arabidopsis thaliana | ATP | 2.18 | 0.171 | 12.8 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant R454K, ATP |
| glutathione synthetase (AtGS)variant R454K | Arabidopsis thaliana | ATP | — | — | 19.01 | 25 °C | Open record: glutathione synthetase (AtGS) variant R454K, ATP |
| glutathione synthetase (AtGS)variant R454K | Arabidopsis thaliana | glycine | 2.47 | 4.17 | 0.592 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant R454K, glycine |
| glutathione synthetase (AtGS)variant R454K | Arabidopsis thaliana | glycine | — | — | 0.637 | 25 °C | Open record: glutathione synthetase (AtGS) variant R454K, glycine |
| glutathione synthetase (AtGS)variant R454K | Arabidopsis thaliana | γ-glutamylcysteine | 3.82 | 0.143 | 26.7 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant R454K, γ-glutamylcysteine |
| glutathione synthetase (AtGS)variant R454K | Arabidopsis thaliana | γ-glutamylcysteine | — | — | 20.42 | 25 °C | Open record: glutathione synthetase (AtGS) variant R454K, γ-glutamylcysteine |
| glutathione synthetase (AtGS)variant S153A | Arabidopsis thaliana | ATP | 5.7 | 0.116 | 49.1 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant S153A, ATP |
| glutathione synthetase (AtGS)variant S153A | Arabidopsis thaliana | glycine | 7.2 | 3.41 | 2.11 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant S153A, glycine |
| glutathione synthetase (AtGS)variant S153A | Arabidopsis thaliana | γ-glutamylcysteine | 5.32 | 0.166 | 32.03 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant S153A, γ-glutamylcysteine |
| glutathione synthetase (AtGS)variant S155A | Arabidopsis thaliana | ATP | 5.08 | 0.189 | 26.9 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant S155A, ATP |
| glutathione synthetase (AtGS)variant S155A | Arabidopsis thaliana | glycine | 5.18 | 2.25 | 2.3 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant S155A, glycine |
| glutathione synthetase (AtGS)variant S155A | Arabidopsis thaliana | γ-glutamylcysteine | 3.52 | 0.035 | 100.6 | pH 7.5, 25 °C | Open record: glutathione synthetase (AtGS) variant S155A, γ-glutamylcysteine |