CatLogenzyme kinetics catalog

Values CatLog read from this paper

Probing the origins of glutathione biosynthesis through biochemical analysis of glutamate-cysteine ligase and glutathione synthetase from a model photosynthetic prokaryote

PMID 23170977doi:10.1042/bj20121332Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 21 records (61 values) from this paper: glutamate-cysteine ligase (SynGCL), glutathione synthetase (SynGS) from Synechocystis sp. PCC 6803.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 6.3.2.2, EC 6.3.2.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
glutamate-cysteine ligase (SynGCL)Synechocystis sp. PCC 6803ATP0.510.1453.52pH 7.5, 25 °COpen
glutamate-cysteine ligase (SynGCL)Synechocystis sp. PCC 6803Cysteine0.420.0597.12pH 7.5, 25 °COpen
glutamate-cysteine ligase (SynGCL)Synechocystis sp. PCC 6803Glutamate0.350.9530.367pH 7.5, 25 °COpen
glutamate-cysteine ligase (SynGCL)variant H121ASynechocystis sp. PCC 6803ATP0.0980.310.317pH 7.5, 25 °COpen
glutamate-cysteine ligase (SynGCL)variant H121ASynechocystis sp. PCC 6803Cysteine0.090.7070.127pH 7.5, 25 °COpen
glutamate-cysteine ligase (SynGCL)variant H121ASynechocystis sp. PCC 6803Glutamate0.12715.20.0083pH 7.5, 25 °COpen
glutamate-cysteine ligase (SynGCL)variant H121QSynechocystis sp. PCC 6803ATP0.3820.2791.37pH 7.5, 25 °COpen
glutamate-cysteine ligase (SynGCL)variant H121QSynechocystis sp. PCC 6803Cysteine0.3350.1162.89pH 7.5, 25 °COpen
glutamate-cysteine ligase (SynGCL)variant H121QSynechocystis sp. PCC 6803Glutamate0.1881.180.16pH 7.5, 25 °COpen
glutamate-cysteine ligase (SynGCL)variant R167KSynechocystis sp. PCC 6803ATP0.0380.4450.0861pH 7.5, 25 °COpen
glutamate-cysteine ligase (SynGCL)variant R167KSynechocystis sp. PCC 6803Cysteine0.0251.950.0128pH 7.5, 25 °COpen
glutamate-cysteine ligase (SynGCL)variant R248KSynechocystis sp. PCC 6803ATP0.00830.9690.0086pH 7.5, 25 °COpen
glutamate-cysteine ligase (SynGCL)variant R248KSynechocystis sp. PCC 6803Cysteine0.010.8570.0117pH 7.5, 25 °COpen
glutamate-cysteine ligase (SynGCL)variant R248KSynechocystis sp. PCC 6803Glutamate0.00833.760.0022pH 7.5, 25 °COpen
glutamate-cysteine ligase (SynGCL)variant T117SSynechocystis sp. PCC 6803ATP0.2250.3520.639pH 7.5, 25 °COpen
glutamate-cysteine ligase (SynGCL)variant T117SSynechocystis sp. PCC 6803Cysteine0.1330.1860.717pH 7.5, 25 °COpen
glutamate-cysteine ligase (SynGCL)variant T117SSynechocystis sp. PCC 6803Glutamate0.1376.10.0224pH 7.5, 25 °COpen
glutathione synthetase (SynGS)Synechocystis sp. PCC 6803ATP5.40.13639.71pH 7.5, 25 °COpen
glutathione synthetase (SynGS)Synechocystis sp. PCC 6803Glycine4.220.40710.36pH 7.5, 25 °COpen
glutathione synthetase (SynGS)Synechocystis sp. PCC 6803γ-Glutamylcysteinevaried substrate with the other two fixed at saturation4.620.09946.63pH 7.5, 25 °COpen
glutathione synthetase (SynGS)Synechocystis sp. PCC 6803γ-Glutamylcysteinematrix of γ-glutamylcysteine 0.05-0.5 mM; ATP 0.1-1.2 mM3.07——pH 7.5, 25 °COpen