Values CatLog read from this paper
Escherichia coli gamma-glutamylcysteine synthetase. Two active site metal ions affect substrate and inhibitor binding
PMID 11675389doi:10.1074/jbc.m107961200Open in Browse
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CatLog read 46 records (46 values) from this paper: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | D-glutamate25 mM MgCl2 | — | 75 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), D-glutamate |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | D-glutamate10 mM MnCl2 | — | 5.9 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), D-glutamate |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | glycine25 mM MgCl2 | — | 17.6 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), glycine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | glycine10 mM MnCl2 | — | 1.7 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), glycine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-2-aminobutanoate25 mM MgCl2 | — | 3.9 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-2-aminobutanoate |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-2-aminobutanoate10 mM MnCl2 | — | 0.41 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-2-aminobutanoate |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-2-aminopent-4-enoic acid25 mM MgCl2 | — | 6.9 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-2-aminopent-4-enoic acid |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-2-aminopent-4-enoic acid10 mM MnCl2 | — | 4.2 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-2-aminopent-4-enoic acid |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-alanine25 mM MgCl2 | — | 21.7 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-alanine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-alanine10 mM MnCl2 | — | 7.8 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-alanine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-allo-threonine25 mM MgCl2 | — | 18.8 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-allo-threonine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-allo-threonine10 mM MnCl2 | — | 4.4 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-allo-threonine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-cysteine25 mM MgCl2 | — | 0.1 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-cysteine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-cysteine10 mM MnCl2 | — | 0.045 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-cysteine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-glutamate25 mM MgCl2 | — | 1.9 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-glutamate |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-glutamate10 mM MnCl2 | — | 0.32 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-glutamate |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-isoleucine25 mM MgCl2 | — | 23 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-isoleucine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-isoleucine10 mM MnCl2 | — | 9.3 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-isoleucine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-leucine25 mM MgCl2 | — | 57.9 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-leucine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-leucine10 mM MnCl2 | — | 19 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-leucine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-norleucine25 mM MgCl2 | — | 33.3 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-norleucine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-norleucine10 mM MnCl2 | — | 12.6 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-norleucine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-norvaline25 mM MgCl2 | — | 61 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-norvaline |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-norvaline10 mM MnCl2 | — | 16.3 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-norvaline |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-serine25 mM MgCl2 | — | 24.6 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-serine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-serine10 mM MnCl2 | — | 7.6 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-serine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-threonine25 mM MgCl2 | — | 73.9 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-threonine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-threonine10 mM MnCl2 | — | 114 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-threonine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-valine25 mM MgCl2 | — | 27.1 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-valine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | L-valine10 mM MnCl2 | — | 21 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), L-valine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | N-methyl-L-glutamate25 mM MgCl2 | — | 27.6 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), N-methyl-L-glutamate |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | N-methyl-L-glutamate10 mM MnCl2 | — | 0.37 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), N-methyl-L-glutamate |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | O-methyl-D,L-serine25 mM MgCl2 | — | 31.1 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), O-methyl-D,L-serine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | O-methyl-D,L-serine10 mM MnCl2 | — | 6.1 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), O-methyl-D,L-serine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | S-methyl-L-cysteine25 mM MgCl2 | — | 8.1 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), S-methyl-L-cysteine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | S-methyl-L-cysteine10 mM MnCl2 | — | 0.32 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), S-methyl-L-cysteine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | α-ethyl-L-glutamate25 mM MgCl2 | — | 18.2 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), α-ethyl-L-glutamate |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | α-ethyl-L-glutamate10 mM MnCl2 | — | 0.53 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), α-ethyl-L-glutamate |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | α-methyl-L-glutamate25 mM MgCl2 | — | 3.9 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), α-methyl-L-glutamate |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | α-methyl-L-glutamate10 mM MnCl2 | — | 0.41 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), α-methyl-L-glutamate |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | β-amino-iso-butyrate25 mM MgCl2 | — | 29.9 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), β-amino-iso-butyrate |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | β-amino-iso-butyrate10 mM MnCl2 | — | 6.1 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), β-amino-iso-butyrate |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | β-chloro-L-alanine25 mM MgCl2 | — | 0.17 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), β-chloro-L-alanine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | β-chloro-L-alanine10 mM MnCl2 | — | 0.031 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), β-chloro-L-alanine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | β-cyano-L-alanine25 mM MgCl2 | — | 0.28 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), β-cyano-L-alanine |
| γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase) | Escherichia coli | β-cyano-L-alanine10 mM MnCl2 | — | 0.028 | — | pH 8.2, 37 °C | Open record: γ-glutamylcysteine synthetase (γ-GCS, glutamate-cysteine ligase), β-cyano-L-alanine |