Values CatLog read from this paper
Changing the amino acid specificity of yeast tyrosyl-tRNA synthetase by genetic engineering
PMID 11530018doi:10.1093/oxfordjournals.jbchem.a003001Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (35 values) from this paper: tyrosyl-tRNA synthetase (TyrRS) from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| tyrosyl-tRNA synthetase (TyrRS) | Saccharomyces cerevisiae | 3-chloro-L-tyrosine | 0.13 | 2.04 | 0.065 | 30 °C | Open record: tyrosyl-tRNA synthetase (TyrRS), 3-chloro-L-tyrosine |
| tyrosyl-tRNA synthetase (TyrRS) | Saccharomyces cerevisiae | 3-fluoro-DL-tyrosine | 4.96 | 0.625 | 7.97 | 30 °C | Open record: tyrosyl-tRNA synthetase (TyrRS), 3-fluoro-DL-tyrosine |
| tyrosyl-tRNA synthetase (TyrRS) | Saccharomyces cerevisiae | D-tyrosine | 1.2 | 0.459 | 2.62 | 30 °C | Open record: tyrosyl-tRNA synthetase (TyrRS), D-tyrosine |
| tyrosyl-tRNA synthetase (TyrRS) | Saccharomyces cerevisiae | L-3,4-dihydroxyphenylalanine | 1.65 | 1.84 | 0.895 | 30 °C | Open record: tyrosyl-tRNA synthetase (TyrRS), L-3,4-dihydroxyphenylalanine |
| tyrosyl-tRNA synthetase (TyrRS) | Saccharomyces cerevisiae | L-tyrosine | 4.37 | 0.012 | 360 calc. | Open record: tyrosyl-tRNA synthetase (TyrRS), L-tyrosine | |
| tyrosyl-tRNA synthetase (TyrRS) | Saccharomyces cerevisiae | L-tyrosine | 4.6 | 0.014 | 321 | 30 °C | Open record: tyrosyl-tRNA synthetase (TyrRS), L-tyrosine |
| tyrosyl-tRNA synthetase (TyrRS)variant Y43G | Saccharomyces cerevisiae | 3-chloro-L-tyrosine | 1.46 | 2.43 | 0.602 | 30 °C | Open record: tyrosyl-tRNA synthetase (TyrRS) variant Y43G, 3-chloro-L-tyrosine |
| tyrosyl-tRNA synthetase (TyrRS)variant Y43G | Saccharomyces cerevisiae | 3-fluoro-DL-tyrosine | 0.62 | 1.3 | 0.475 | 30 °C | Open record: tyrosyl-tRNA synthetase (TyrRS) variant Y43G, 3-fluoro-DL-tyrosine |
| tyrosyl-tRNA synthetase (TyrRS)variant Y43G | Saccharomyces cerevisiae | 3-iodo-L-tyrosine | 0.48 | 1.15 | 0.425 | 30 °C | Open record: tyrosyl-tRNA synthetase (TyrRS) variant Y43G, 3-iodo-L-tyrosine |
| tyrosyl-tRNA synthetase (TyrRS)variant Y43G | Saccharomyces cerevisiae | D-tyrosine | 0.37 | 14 | 0.027 | 30 °C | Open record: tyrosyl-tRNA synthetase (TyrRS) variant Y43G, D-tyrosine |
| tyrosyl-tRNA synthetase (TyrRS)variant Y43G | Saccharomyces cerevisiae | L-3,4-dihydroxyphenylalanine | 0.67 | 0.56 | 1.02 | 30 °C | Open record: tyrosyl-tRNA synthetase (TyrRS) variant Y43G, L-3,4-dihydroxyphenylalanine |
| tyrosyl-tRNA synthetase (TyrRS)variant Y43G | Saccharomyces cerevisiae | L-tyrosine | 0.95 | 1.19 | 0.804 | 30 °C | Open record: tyrosyl-tRNA synthetase (TyrRS) variant Y43G, L-tyrosine |