Values CatLog read from this paper
Hydrophobic amino acid residues in the acceptor binding site are main determinants for reaction mechanism and specificity of cyclodextrin-glycosyltransferase
PMID 11555657doi:10.1074/jbc.m107533200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 32 records (32 values) from this paper: cyclodextrin-glycosyltransferase from Bacillus circulans.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cyclodextrin-glycosyltransferase | Bacillus circulans | 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene | — | 0.22 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase, 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene |
| cyclodextrin-glycosyltransferase | Bacillus circulans | beta-cyclodextrin | — | 0.32 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase, beta-cyclodextrin |
| cyclodextrin-glycosyltransferase | Bacillus circulans | maltose | — | 0.83 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase, maltose |
| cyclodextrin-glycosyltransferase | Bacillus circulans | methyl alpha-D-glucopyranoside | — | 18.1 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase, methyl alpha-D-glucopyranoside |
| cyclodextrin-glycosyltransferasevariant E264A | Bacillus circulans | 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene | — | 0.11 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant E264A, 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene |
| cyclodextrin-glycosyltransferasevariant E264A | Bacillus circulans | beta-cyclodextrin | — | 0.24 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant E264A, beta-cyclodextrin |
| cyclodextrin-glycosyltransferasevariant E264A | Bacillus circulans | maltose | — | 0.1 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant E264A, maltose |
| cyclodextrin-glycosyltransferasevariant E264A | Bacillus circulans | methyl alpha-D-glucopyranoside | — | 17.8 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant E264A, methyl alpha-D-glucopyranoside |
| cyclodextrin-glycosyltransferasevariant F183N | Bacillus circulans | 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene | — | 0.26 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant F183N, 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene |
| cyclodextrin-glycosyltransferasevariant F183N | Bacillus circulans | beta-cyclodextrin | — | 0.15 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant F183N, beta-cyclodextrin |
| cyclodextrin-glycosyltransferasevariant F183N | Bacillus circulans | maltose | — | 0.31 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant F183N, maltose |
| cyclodextrin-glycosyltransferasevariant F183N | Bacillus circulans | methyl alpha-D-glucopyranoside | — | 6.2 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant F183N, methyl alpha-D-glucopyranoside |
| cyclodextrin-glycosyltransferasevariant F183S | Bacillus circulans | 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene | — | 0.27 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant F183S, 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene |
| cyclodextrin-glycosyltransferasevariant F183S | Bacillus circulans | beta-cyclodextrin | — | 0.41 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant F183S, beta-cyclodextrin |
| cyclodextrin-glycosyltransferasevariant F183S | Bacillus circulans | maltose | — | 0.27 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant F183S, maltose |
| cyclodextrin-glycosyltransferasevariant F183S | Bacillus circulans | methyl alpha-D-glucopyranoside | — | 4.7 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant F183S, methyl alpha-D-glucopyranoside |
| cyclodextrin-glycosyltransferasevariant F183S/F259N | Bacillus circulans | 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene | — | 0.7 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant F183S/F259N, 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene |
| cyclodextrin-glycosyltransferasevariant F183S/F259N | Bacillus circulans | maltose | — | 0.01 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant F183S/F259N, maltose |
| cyclodextrin-glycosyltransferasevariant F259N | Bacillus circulans | 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene | — | 0.1 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant F259N, 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene |
| cyclodextrin-glycosyltransferasevariant F259N | Bacillus circulans | beta-cyclodextrin | — | 0.38 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant F259N, beta-cyclodextrin |
| cyclodextrin-glycosyltransferasevariant F259N | Bacillus circulans | maltose | — | 0.14 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant F259N, maltose |
| cyclodextrin-glycosyltransferasevariant F259N | Bacillus circulans | methyl alpha-D-glucopyranoside | — | 17.7 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant F259N, methyl alpha-D-glucopyranoside |
| cyclodextrin-glycosyltransferasevariant F259S | Bacillus circulans | beta-cyclodextrin | — | 0.54 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant F259S, beta-cyclodextrin |
| cyclodextrin-glycosyltransferasevariant F259S | Bacillus circulans | methyl alpha-D-glucopyranoside | — | 79.5 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant F259S, methyl alpha-D-glucopyranoside |
| cyclodextrin-glycosyltransferasevariant K232L | Bacillus circulans | 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene | — | 0.29 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant K232L, 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene |
| cyclodextrin-glycosyltransferasevariant K232L | Bacillus circulans | beta-cyclodextrin | — | 0.47 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant K232L, beta-cyclodextrin |
| cyclodextrin-glycosyltransferasevariant K232L | Bacillus circulans | maltose | — | 0.42 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant K232L, maltose |
| cyclodextrin-glycosyltransferasevariant K232L | Bacillus circulans | methyl alpha-D-glucopyranoside | — | 44.8 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant K232L, methyl alpha-D-glucopyranoside |
| cyclodextrin-glycosyltransferasevariant K232Q | Bacillus circulans | 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene | — | 0.39 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant K232Q, 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene |
| cyclodextrin-glycosyltransferasevariant K232Q | Bacillus circulans | beta-cyclodextrin | — | 0.49 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant K232Q, beta-cyclodextrin |
| cyclodextrin-glycosyltransferasevariant K232Q | Bacillus circulans | maltose | — | 0.29 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant K232Q, maltose |
| cyclodextrin-glycosyltransferasevariant K232Q | Bacillus circulans | methyl alpha-D-glucopyranoside | — | 60.6 | — | pH 6, 50 °C | Open record: cyclodextrin-glycosyltransferase variant K232Q, methyl alpha-D-glucopyranoside |