EC 2.4.1.19: cyclomaltodextrin glucanotransferase

TransferasesOpen in Browse

CatLog read 134 records for EC 2.4.1.19 from 6 papers, for 6 organisms. Names in these papers: cyclodextrin glucanotransferase (CGTase), cyclodextrin-glycosyltransferase, cyclodextrin glucosyltransferase (CGTase), cyclodextrin glycosyltransferase (CGTase) and 2 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 66 records for EC 2.4.1.19 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.4.1.19 in Browse.

Papers

Papers with records for EC 2.4.1.19
Paper	Organisms	Records
Four aromatic residues in the active center of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. 1011: effects of replacements on substrate binding and cyclization characteristics	Bacillus sp.	55
Hydrophobic amino acid residues in the acceptor binding site are main determinants for reaction mechanism and specificity of cyclodextrin-glycosyltransferase	Bacillus circulans	32
Effect of Leu²⁷⁷ on Disproportionation and Hydrolysis Activity in Bacillus stearothermophilus NO2 Cyclodextrin Glucosyltransferase	Bacillus stearothermophilus	18
Systems engineering of tyrosine 195, tyrosine 260, and glutamine 265 in cyclodextrin glycosyltransferase from Paenibacillus macerans to enhance maltodextrin specificity for…	Paenibacillus macerans	16
Thermoanaerobacterium thermosulfurigenes cyclodextrin glycosyltransferase	Thermoanaerobacterium thermosulfurigenes	12
Efficient glycosylation of polyphenols via dynamic complexation of cyclodextrin and synchronous coupling reaction with cyclodextrin glycosyltransferase in water	Bacillus sp. (alkalophilic)	1

Records

Records for EC 2.4.1.19 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
cyclodextrin glucanotransferase (CGTase)	Bacillus sp.	3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor glucose); cosubstrate glucose	244	0.2	1.2 × 10³ calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase), 3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside
cyclodextrin glucanotransferase (CGTase)	Bacillus sp.	3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor maltose); cosubstrate maltose	203	0.16	1.3 × 10³ calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase), 3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside
cyclodextrin glucanotransferase (CGTase)	Bacillus sp.	alpha-cyclodextrin	2.65	0.57	4.6 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase), alpha-cyclodextrin
cyclodextrin glucanotransferase (CGTase)	Bacillus sp.	amylose (average degree of polymerization of 17)	30.5	0.0574	531 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase), amylose (average degree of polymerization of 17)
cyclodextrin glucanotransferase (CGTase)	Bacillus sp.	beta-cyclodextrin	0.57	0.11	5.2 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase), beta-cyclodextrin
cyclodextrin glucanotransferase (CGTase)	Bacillus sp.	D-glucose	—	11.3	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase), D-glucose
cyclodextrin glucanotransferase (CGTase)	Bacillus sp.	gamma-cyclodextrin	67.8	0.12	560 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase), gamma-cyclodextrin
cyclodextrin glucanotransferase (CGTase)	Bacillus sp.	maltose	—	0.49	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase), maltose
cyclodextrin glucanotransferase (CGTase)	Bacillus sp.	methyl alpha-D-glucopyranoside	—	16.9	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase), methyl alpha-D-glucopyranoside
cyclodextrin glucanotransferase (CGTase)variant F183L	Bacillus sp.	3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor glucose); cosubstrate glucose	928	0.23	4.0 × 10³ calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F183L, 3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside
cyclodextrin glucanotransferase (CGTase)variant F183L	Bacillus sp.	3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor maltose); cosubstrate maltose	333	0.08	4.2 × 10³ calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F183L, 3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside
cyclodextrin glucanotransferase (CGTase)variant F183L	Bacillus sp.	alpha-cyclodextrin	1.1	1.15	0.96 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F183L, alpha-cyclodextrin
cyclodextrin glucanotransferase (CGTase)variant F183L	Bacillus sp.	amylose (average degree of polymerization of 17)	7.3	0.0815	90 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F183L, amylose (average degree of polymerization of 17)
cyclodextrin glucanotransferase (CGTase)variant F183L	Bacillus sp.	D-glucose	—	57.8	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F183L, D-glucose
cyclodextrin glucanotransferase (CGTase)variant F183L	Bacillus sp.	gamma-cyclodextrin	49.1	0.21	230 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F183L, gamma-cyclodextrin
cyclodextrin glucanotransferase (CGTase)variant F183L	Bacillus sp.	maltose	—	1.42	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F183L, maltose
cyclodextrin glucanotransferase (CGTase)variant F183L	Bacillus sp.	methyl alpha-D-glucopyranoside	—	44	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F183L, methyl alpha-D-glucopyranoside
cyclodextrin glucanotransferase (CGTase)variant F183L/F259L	Bacillus sp.	3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor glucose); cosubstrate glucose	228	0.34	670 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F183L/F259L, 3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside
cyclodextrin glucanotransferase (CGTase)variant F183L/F259L	Bacillus sp.	3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor maltose); cosubstrate maltose	158	0.24	660 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F183L/F259L, 3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside
cyclodextrin glucanotransferase (CGTase)variant F183L/F259L	Bacillus sp.	amylose (average degree of polymerization of 17)	0.16	0.263	0.61 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F183L/F259L, amylose (average degree of polymerization of 17)
cyclodextrin glucanotransferase (CGTase)variant F183L/F259L	Bacillus sp.	D-glucose	—	160	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F183L/F259L, D-glucose
cyclodextrin glucanotransferase (CGTase)variant F183L/F259L	Bacillus sp.	maltose	—	18.2	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F183L/F259L, maltose
cyclodextrin glucanotransferase (CGTase)variant F259L	Bacillus sp.	3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor glucose); cosubstrate glucose	20.7	0.56	37 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F259L, 3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside
cyclodextrin glucanotransferase (CGTase)variant F259L	Bacillus sp.	3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor maltose); cosubstrate maltose	25.4	0.48	53 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F259L, 3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside
cyclodextrin glucanotransferase (CGTase)variant F259L	Bacillus sp.	alpha-cyclodextrin	0.72	1.25	0.58 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F259L, alpha-cyclodextrin
cyclodextrin glucanotransferase (CGTase)variant F259L	Bacillus sp.	amylose (average degree of polymerization of 17)	8.2	0.0635	130 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F259L, amylose (average degree of polymerization of 17)
cyclodextrin glucanotransferase (CGTase)variant F259L	Bacillus sp.	D-glucose	—	12	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F259L, D-glucose
cyclodextrin glucanotransferase (CGTase)variant F259L	Bacillus sp.	gamma-cyclodextrin	10.7	0.35	31 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F259L, gamma-cyclodextrin
cyclodextrin glucanotransferase (CGTase)variant F259L	Bacillus sp.	maltose	—	1.3	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F259L, maltose
cyclodextrin glucanotransferase (CGTase)variant F259L	Bacillus sp.	methyl alpha-D-glucopyranoside	—	11.7	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F259L, methyl alpha-D-glucopyranoside
cyclodextrin glucanotransferase (CGTase)variant F283L	Bacillus sp.	3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor glucose); cosubstrate glucose	7.3	0.15	49 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F283L, 3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside
cyclodextrin glucanotransferase (CGTase)variant F283L	Bacillus sp.	3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor maltose); cosubstrate maltose	8.2	0.12	68 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F283L, 3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside
cyclodextrin glucanotransferase (CGTase)variant F283L	Bacillus sp.	alpha-cyclodextrin	0.23	0.94	0.24 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F283L, alpha-cyclodextrin
cyclodextrin glucanotransferase (CGTase)variant F283L	Bacillus sp.	amylose (average degree of polymerization of 17)	12	0.0724	170 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F283L, amylose (average degree of polymerization of 17)
cyclodextrin glucanotransferase (CGTase)variant F283L	Bacillus sp.	D-glucose	—	7.3	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F283L, D-glucose
cyclodextrin glucanotransferase (CGTase)variant F283L	Bacillus sp.	gamma-cyclodextrin	2.1	0.1	21 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F283L, gamma-cyclodextrin
cyclodextrin glucanotransferase (CGTase)variant F283L	Bacillus sp.	maltose	—	0.17	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F283L, maltose
cyclodextrin glucanotransferase (CGTase)variant F283L	Bacillus sp.	methyl alpha-D-glucopyranoside	—	39.9	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant F283L, methyl alpha-D-glucopyranoside
cyclodextrin glucanotransferase (CGTase)variant Y195F	Bacillus sp.	3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor glucose); cosubstrate glucose	139	0.2	700 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant Y195F, 3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside
cyclodextrin glucanotransferase (CGTase)variant Y195F	Bacillus sp.	3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor maltose); cosubstrate maltose	192	0.22	870 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant Y195F, 3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside
cyclodextrin glucanotransferase (CGTase)variant Y195F	Bacillus sp.	alpha-cyclodextrin	1.53	0.55	2.8 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant Y195F, alpha-cyclodextrin
cyclodextrin glucanotransferase (CGTase)variant Y195F	Bacillus sp.	amylose (average degree of polymerization of 17)	28.9	0.0556	520 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant Y195F, amylose (average degree of polymerization of 17)
cyclodextrin glucanotransferase (CGTase)variant Y195F	Bacillus sp.	D-glucose	—	15.9	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant Y195F, D-glucose
cyclodextrin glucanotransferase (CGTase)variant Y195F	Bacillus sp.	gamma-cyclodextrin	29.2	0.1	290 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant Y195F, gamma-cyclodextrin
cyclodextrin glucanotransferase (CGTase)variant Y195F	Bacillus sp.	maltose	—	0.59	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant Y195F, maltose
cyclodextrin glucanotransferase (CGTase)variant Y195F	Bacillus sp.	methyl alpha-D-glucopyranoside	—	17.4	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant Y195F, methyl alpha-D-glucopyranoside
cyclodextrin glucanotransferase (CGTase)variant Y195L	Bacillus sp.	3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor glucose); cosubstrate glucose	118	0.34	350 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant Y195L, 3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside
cyclodextrin glucanotransferase (CGTase)variant Y195L	Bacillus sp.	3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor maltose); cosubstrate maltose	132	0.31	430 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant Y195L, 3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside
cyclodextrin glucanotransferase (CGTase)variant Y195L	Bacillus sp.	alpha-cyclodextrin	0.11	2.56	0.043 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant Y195L, alpha-cyclodextrin
cyclodextrin glucanotransferase (CGTase)variant Y195L	Bacillus sp.	amylose (average degree of polymerization of 17)	8.9	0.0618	140 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant Y195L, amylose (average degree of polymerization of 17)
cyclodextrin glucanotransferase (CGTase)variant Y195L	Bacillus sp.	beta-cyclodextrin	0.84	0.44	1.9 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant Y195L, beta-cyclodextrin
cyclodextrin glucanotransferase (CGTase)variant Y195L	Bacillus sp.	D-glucose	—	9.8	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant Y195L, D-glucose
cyclodextrin glucanotransferase (CGTase)variant Y195L	Bacillus sp.	gamma-cyclodextrin	18.9	0.51	37 calc.	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant Y195L, gamma-cyclodextrin
cyclodextrin glucanotransferase (CGTase)variant Y195L	Bacillus sp.	maltose	—	0.38	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant Y195L, maltose
cyclodextrin glucanotransferase (CGTase)variant Y195L	Bacillus sp.	methyl alpha-D-glucopyranoside	—	12	—	pH 6.5, 37 °C	PMID 8061001	Open record: cyclodextrin glucanotransferase (CGTase) variant Y195L, methyl alpha-D-glucopyranoside
cyclodextrin glucosyltransferase (CGTase)	Bacillus stearothermophilus	maltose	648.3	0.945	686.1	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase), maltose
cyclodextrin glucosyltransferase (CGTase)	Bacillus stearothermophilus	soluble starch	11	—	—	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase), soluble starch
cyclodextrin glucosyltransferase (CGTase)variant G282A	Bacillus stearothermophilus	maltose	526.8	0.648	813.5	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase) variant G282A, maltose
cyclodextrin glucosyltransferase (CGTase)variant G282A	Bacillus stearothermophilus	soluble starch	16.54	—	—	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase) variant G282A, soluble starch
cyclodextrin glucosyltransferase (CGTase)variant L277A	Bacillus stearothermophilus	maltose	247.3	2.24	110.2	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase) variant L277A, maltose
cyclodextrin glucosyltransferase (CGTase)variant L277A	Bacillus stearothermophilus	soluble starch	27.18	—	—	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase) variant L277A, soluble starch
cyclodextrin glucosyltransferase (CGTase)variant L277F	Bacillus stearothermophilus	maltose	375.3	0.49	773.8	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase) variant L277F, maltose
cyclodextrin glucosyltransferase (CGTase)variant L277F	Bacillus stearothermophilus	soluble starch	4.91	—	—	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase) variant L277F, soluble starch
cyclodextrin glucosyltransferase (CGTase)variant L277F/G282A	Bacillus stearothermophilus	maltose	496.2	0.58	860	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase) variant L277F/G282A, maltose
cyclodextrin glucosyltransferase (CGTase)variant L277F/G282A	Bacillus stearothermophilus	soluble starch	10.87	—	—	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase) variant L277F/G282A, soluble starch
cyclodextrin glucosyltransferase (CGTase)variant L277I	Bacillus stearothermophilus	maltose	478.4	0.81	594.3	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase) variant L277I, maltose
cyclodextrin glucosyltransferase (CGTase)variant L277I	Bacillus stearothermophilus	soluble starch	11.64	—	—	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase) variant L277I, soluble starch
cyclodextrin glucosyltransferase (CGTase)variant L277M	Bacillus stearothermophilus	maltose	564.1	0.48	1175	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase) variant L277M, maltose
cyclodextrin glucosyltransferase (CGTase)variant L277M	Bacillus stearothermophilus	soluble starch	3.24	—	—	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase) variant L277M, soluble starch
cyclodextrin glucosyltransferase (CGTase)variant L277M/G282A	Bacillus stearothermophilus	maltose	621.3	1.22	507.6	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase) variant L277M/G282A, maltose
cyclodextrin glucosyltransferase (CGTase)variant L277M/G282A	Bacillus stearothermophilus	soluble starch	4.04	—	—	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase) variant L277M/G282A, soluble starch
cyclodextrin glucosyltransferase (CGTase)variant L277T	Bacillus stearothermophilus	maltose	200.9	0.84	239.6	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase) variant L277T, maltose
cyclodextrin glucosyltransferase (CGTase)variant L277T	Bacillus stearothermophilus	soluble starch	6.18	—	—	pH 5.5, 50 °C	PMID 33837009	Open record: cyclodextrin glucosyltransferase (CGTase) variant L277T, soluble starch
cyclodextrin glycosyltransferase	Thermoanaerobacterium thermosulfurigenes	4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene	1.2 × 10³	0.21	5714	pH 6, 60 °C	PMID 12492486	Open record: cyclodextrin glycosyltransferase, 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene
cyclodextrin glycosyltransferase	Thermoanaerobacterium thermosulfurigenes	glucose	1019	11.7	87.1 calc.	pH 6, 60 °C	PMID 12492486	Open record: cyclodextrin glycosyltransferase, glucose
cyclodextrin glycosyltransferase	Thermoanaerobacterium thermosulfurigenes	maltosecosubstrate EPS	—	4.6	—	pH 6, 60 °C	PMID 12492486	Open record: cyclodextrin glycosyltransferase, maltose
cyclodextrin glycosyltransferase	Thermoanaerobacterium thermosulfurigenes	maltosedonor EPS fixed at 2 mM; cosubstrate EPS (2 mM)	1.2 × 10³	4.6	260 calc.	pH 6, 60 °C	PMID 12492486	Open record: cyclodextrin glycosyltransferase, maltose
cyclodextrin glycosyltransferase	Thermoanaerobacterium thermosulfurigenes	maltotetraose	1132	1.1	1.0 × 10³ calc.	pH 6, 60 °C	PMID 12492486	Open record: cyclodextrin glycosyltransferase, maltotetraose
cyclodextrin glycosyltransferase	Thermoanaerobacterium thermosulfurigenes	maltotriose	1115	1.2	930 calc.	pH 6, 60 °C	PMID 12492486	Open record: cyclodextrin glycosyltransferase, maltotriose
cyclodextrin glycosyltransferase	Thermoanaerobacterium thermosulfurigenes	methyl alpha-D-glucopyranosidealpha-cyclodextrin; cosubstrate α-cyclodextrin	—	20	—	pH 6, 60 °C	PMID 12492486	Open record: cyclodextrin glycosyltransferase, methyl alpha-D-glucopyranoside
cyclodextrin glycosyltransferase	Thermoanaerobacterium thermosulfurigenes	methyl alpha-D-glucopyranosidebeta-cyclodextrin; cosubstrate β-cyclodextrin	—	83	—	pH 6, 60 °C	PMID 12492486	Open record: cyclodextrin glycosyltransferase, methyl alpha-D-glucopyranoside
cyclodextrin glycosyltransferase	Thermoanaerobacterium thermosulfurigenes	methyl alpha-D-glucopyranosidegamma-cyclodextrin; cosubstrate γ-cyclodextrin	—	45	—	pH 6, 60 °C	PMID 12492486	Open record: cyclodextrin glycosyltransferase, methyl alpha-D-glucopyranoside
cyclodextrin glycosyltransferase	Thermoanaerobacterium thermosulfurigenes	α-cyclodextrin	1586	1.1	1.4 × 10³ calc.	pH 6, 60 °C	PMID 12492486	Open record: cyclodextrin glycosyltransferase, α-cyclodextrin
cyclodextrin glycosyltransferase	Thermoanaerobacterium thermosulfurigenes	β-cyclodextrin	509	1	510 calc.	pH 6, 60 °C	PMID 12492486	Open record: cyclodextrin glycosyltransferase, β-cyclodextrin
cyclodextrin glycosyltransferase	Thermoanaerobacterium thermosulfurigenes	γ-cyclodextrin	588	1.5	390 calc.	pH 6, 60 °C	PMID 12492486	Open record: cyclodextrin glycosyltransferase, γ-cyclodextrin
cyclodextrin glycosyltransferase (CGTase)	Paenibacillus macerans	L-ascorbic acid	—	38.32	9.7 × 10⁻⁵	pH 5.5, 37 °C	PMID 23160123	Open record: cyclodextrin glycosyltransferase (CGTase), L-ascorbic acid
cyclodextrin glycosyltransferase (CGTase)	Paenibacillus macerans	maltodextrin	0.00375	0.64	0.005858	pH 5.5, 37 °C	PMID 23160123	Open record: cyclodextrin glycosyltransferase (CGTase), maltodextrin
cyclodextrin glycosyltransferase (CGTase)variant Q265K	Paenibacillus macerans	L-ascorbic acid	—	44.32	1 × 10⁻⁴	pH 5.5, 37 °C	PMID 23160123	Open record: cyclodextrin glycosyltransferase (CGTase) variant Q265K, L-ascorbic acid
cyclodextrin glycosyltransferase (CGTase)variant Q265K	Paenibacillus macerans	maltodextrin	0.004375	0.52	0.008411	pH 5.5, 37 °C	PMID 23160123	Open record: cyclodextrin glycosyltransferase (CGTase) variant Q265K, maltodextrin
cyclodextrin glycosyltransferase (CGTase)variant Q265K/Y195S	Paenibacillus macerans	L-ascorbic acid	—	47.21	1 × 10⁻⁴	pH 5.5, 37 °C	PMID 23160123	Open record: cyclodextrin glycosyltransferase (CGTase) variant Q265K/Y195S, L-ascorbic acid
cyclodextrin glycosyltransferase (CGTase)variant Q265K/Y195S	Paenibacillus macerans	maltodextrin	0.004792	0.5	0.00958	pH 5.5, 37 °C	PMID 23160123	Open record: cyclodextrin glycosyltransferase (CGTase) variant Q265K/Y195S, maltodextrin
cyclodextrin glycosyltransferase (CGTase)variant Y195S	Paenibacillus macerans	L-ascorbic acid	—	48.47	8.6 × 10⁻⁵	pH 5.5, 37 °C	PMID 23160123	Open record: cyclodextrin glycosyltransferase (CGTase) variant Y195S, L-ascorbic acid
cyclodextrin glycosyltransferase (CGTase)variant Y195S	Paenibacillus macerans	maltodextrin	0.00417	0.62	0.006719	pH 5.5, 37 °C	PMID 23160123	Open record: cyclodextrin glycosyltransferase (CGTase) variant Y195S, maltodextrin
cyclodextrin glycosyltransferase (CGTase)variant Y260R	Paenibacillus macerans	L-ascorbic acid	—	43.44	1.06 × 10⁻⁴	pH 5.5, 37 °C	PMID 23160123	Open record: cyclodextrin glycosyltransferase (CGTase) variant Y260R, L-ascorbic acid
cyclodextrin glycosyltransferase (CGTase)variant Y260R	Paenibacillus macerans	maltodextrin	0.004583	0.51	0.008986	pH 5.5, 37 °C	PMID 23160123	Open record: cyclodextrin glycosyltransferase (CGTase) variant Y260R, maltodextrin
cyclodextrin glycosyltransferase (CGTase)variant Y260R/Q265K	Paenibacillus macerans	L-ascorbic acid	—	44.52	1.1 × 10⁻⁴	pH 5.5, 37 °C	PMID 23160123	Open record: cyclodextrin glycosyltransferase (CGTase) variant Y260R/Q265K, L-ascorbic acid
cyclodextrin glycosyltransferase (CGTase)variant Y260R/Q265K	Paenibacillus macerans	maltodextrin	0.005	0.48	0.01042	pH 5.5, 37 °C	PMID 23160123	Open record: cyclodextrin glycosyltransferase (CGTase) variant Y260R/Q265K, maltodextrin
cyclodextrin glycosyltransferase (CGTase)variant Y260R/Q265K/Y195S	Paenibacillus macerans	L-ascorbic acid	—	51.07	1.14 × 10⁻⁴	pH 5.5, 37 °C	PMID 23160123	Open record: cyclodextrin glycosyltransferase (CGTase) variant Y260R/Q265K/Y195S, L-ascorbic acid
cyclodextrin glycosyltransferase (CGTase)variant Y260R/Q265K/Y195S	Paenibacillus macerans	maltodextrin	0.00583	0.47	0.01241	pH 5.5, 37 °C	PMID 23160123	Open record: cyclodextrin glycosyltransferase (CGTase) variant Y260R/Q265K/Y195S, maltodextrin
cyclodextrin glycosyltransferase (CGTase)variant Y260R/Y195S	Paenibacillus macerans	L-ascorbic acid	—	48.84	1.06 × 10⁻⁴	pH 5.5, 37 °C	PMID 23160123	Open record: cyclodextrin glycosyltransferase (CGTase) variant Y260R/Y195S, L-ascorbic acid
cyclodextrin glycosyltransferase (CGTase)variant Y260R/Y195S	Paenibacillus macerans	maltodextrin	0.005208	0.53	0.009828	pH 5.5, 37 °C	PMID 23160123	Open record: cyclodextrin glycosyltransferase (CGTase) variant Y260R/Y195S, maltodextrin
cyclodextrin-glycosyltransferase	Bacillus circulans	4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene	—	0.22	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase, 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene
cyclodextrin-glycosyltransferase	Bacillus circulans	beta-cyclodextrin	—	0.32	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase, beta-cyclodextrin
cyclodextrin-glycosyltransferase	Bacillus circulans	maltose	—	0.83	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase, maltose
cyclodextrin-glycosyltransferase	Bacillus circulans	methyl alpha-D-glucopyranoside	—	18.1	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase, methyl alpha-D-glucopyranoside
cyclodextrin-glycosyltransferasevariant E264A	Bacillus circulans	4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene	—	0.11	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant E264A, 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene
cyclodextrin-glycosyltransferasevariant E264A	Bacillus circulans	beta-cyclodextrin	—	0.24	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant E264A, beta-cyclodextrin
cyclodextrin-glycosyltransferasevariant E264A	Bacillus circulans	maltose	—	0.1	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant E264A, maltose
cyclodextrin-glycosyltransferasevariant E264A	Bacillus circulans	methyl alpha-D-glucopyranoside	—	17.8	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant E264A, methyl alpha-D-glucopyranoside
cyclodextrin-glycosyltransferasevariant F183N	Bacillus circulans	4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene	—	0.26	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant F183N, 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene
cyclodextrin-glycosyltransferasevariant F183N	Bacillus circulans	beta-cyclodextrin	—	0.15	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant F183N, beta-cyclodextrin
cyclodextrin-glycosyltransferasevariant F183N	Bacillus circulans	maltose	—	0.31	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant F183N, maltose
cyclodextrin-glycosyltransferasevariant F183N	Bacillus circulans	methyl alpha-D-glucopyranoside	—	6.2	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant F183N, methyl alpha-D-glucopyranoside
cyclodextrin-glycosyltransferasevariant F183S	Bacillus circulans	4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene	—	0.27	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant F183S, 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene
cyclodextrin-glycosyltransferasevariant F183S	Bacillus circulans	beta-cyclodextrin	—	0.41	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant F183S, beta-cyclodextrin
cyclodextrin-glycosyltransferasevariant F183S	Bacillus circulans	maltose	—	0.27	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant F183S, maltose
cyclodextrin-glycosyltransferasevariant F183S	Bacillus circulans	methyl alpha-D-glucopyranoside	—	4.7	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant F183S, methyl alpha-D-glucopyranoside
cyclodextrin-glycosyltransferasevariant F183S/F259N	Bacillus circulans	4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene	—	0.7	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant F183S/F259N, 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene
cyclodextrin-glycosyltransferasevariant F183S/F259N	Bacillus circulans	maltose	—	0.01	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant F183S/F259N, maltose
cyclodextrin-glycosyltransferasevariant F259N	Bacillus circulans	4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene	—	0.1	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant F259N, 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene
cyclodextrin-glycosyltransferasevariant F259N	Bacillus circulans	beta-cyclodextrin	—	0.38	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant F259N, beta-cyclodextrin
cyclodextrin-glycosyltransferasevariant F259N	Bacillus circulans	maltose	—	0.14	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant F259N, maltose
cyclodextrin-glycosyltransferasevariant F259N	Bacillus circulans	methyl alpha-D-glucopyranoside	—	17.7	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant F259N, methyl alpha-D-glucopyranoside
cyclodextrin-glycosyltransferasevariant F259S	Bacillus circulans	beta-cyclodextrin	—	0.54	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant F259S, beta-cyclodextrin
cyclodextrin-glycosyltransferasevariant F259S	Bacillus circulans	methyl alpha-D-glucopyranoside	—	79.5	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant F259S, methyl alpha-D-glucopyranoside
cyclodextrin-glycosyltransferasevariant K232L	Bacillus circulans	4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene	—	0.29	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant K232L, 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene
cyclodextrin-glycosyltransferasevariant K232L	Bacillus circulans	beta-cyclodextrin	—	0.47	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant K232L, beta-cyclodextrin
cyclodextrin-glycosyltransferasevariant K232L	Bacillus circulans	maltose	—	0.42	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant K232L, maltose
cyclodextrin-glycosyltransferasevariant K232L	Bacillus circulans	methyl alpha-D-glucopyranoside	—	44.8	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant K232L, methyl alpha-D-glucopyranoside
cyclodextrin-glycosyltransferasevariant K232Q	Bacillus circulans	4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene	—	0.39	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant K232Q, 4-nitrophenyl-alpha-D-maltoheptaoside-4,6-O-ethylidene
cyclodextrin-glycosyltransferasevariant K232Q	Bacillus circulans	beta-cyclodextrin	—	0.49	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant K232Q, beta-cyclodextrin
cyclodextrin-glycosyltransferasevariant K232Q	Bacillus circulans	maltose	—	0.29	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant K232Q, maltose
cyclodextrin-glycosyltransferasevariant K232Q	Bacillus circulans	methyl alpha-D-glucopyranoside	—	60.6	—	pH 6, 50 °C	PMID 11555657	Open record: cyclodextrin-glycosyltransferase variant K232Q, methyl alpha-D-glucopyranoside
β-cyclodextrin glycosyltransferase (β-CGTase)	Bacillus sp. (alkalophilic)	γ-cyclodextrin	4.01 × 10³	6.46	621.9		PMID 39353521	Open record: β-cyclodextrin glycosyltransferase (β-CGTase), γ-cyclodextrin