CatLogenzyme kinetics catalog

Values CatLog read from this paper

Four aromatic residues in the active center of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. 1011: effects of replacements on substrate binding and cyclization characteristics

PMID 8061001doi:10.1021/bi00199a015Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 55 records (90 values) from this paper: cyclodextrin glucanotransferase (CGTase) from Bacillus sp..

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 35 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.4.1.19

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
cyclodextrin glucanotransferase (CGTase)Bacillus sp.3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor glucose); cosubstrate glucose2440.21.2 × 10³ calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)Bacillus sp.3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor maltose); cosubstrate maltose2030.161.3 × 10³ calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)Bacillus sp.alpha-cyclodextrin2.650.574.6 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)Bacillus sp.amylose (average degree of polymerization of 17)30.50.0574531 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)Bacillus sp.beta-cyclodextrin0.570.115.2 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)Bacillus sp.D-glucose—11.3—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)Bacillus sp.gamma-cyclodextrin67.80.12560 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)Bacillus sp.maltose—0.49—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)Bacillus sp.methyl alpha-D-glucopyranoside—16.9—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F183LBacillus sp.3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor glucose); cosubstrate glucose9280.234.0 × 10³ calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F183LBacillus sp.3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor maltose); cosubstrate maltose3330.084.2 × 10³ calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F183LBacillus sp.alpha-cyclodextrin1.11.150.96 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F183LBacillus sp.amylose (average degree of polymerization of 17)7.30.081590 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F183LBacillus sp.D-glucose—57.8—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F183LBacillus sp.gamma-cyclodextrin49.10.21230 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F183LBacillus sp.maltose—1.42—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F183LBacillus sp.methyl alpha-D-glucopyranoside—44—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F183L/F259LBacillus sp.3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor glucose); cosubstrate glucose2280.34670 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F183L/F259LBacillus sp.3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor maltose); cosubstrate maltose1580.24660 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F183L/F259LBacillus sp.amylose (average degree of polymerization of 17)0.160.2630.61 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F183L/F259LBacillus sp.D-glucose—160—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F183L/F259LBacillus sp.maltose—18.2—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F259LBacillus sp.3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor glucose); cosubstrate glucose20.70.5637 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F259LBacillus sp.3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor maltose); cosubstrate maltose25.40.4853 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F259LBacillus sp.alpha-cyclodextrin0.721.250.58 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F259LBacillus sp.amylose (average degree of polymerization of 17)8.20.0635130 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F259LBacillus sp.D-glucose—12—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F259LBacillus sp.gamma-cyclodextrin10.70.3531 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F259LBacillus sp.maltose—1.3—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F259LBacillus sp.methyl alpha-D-glucopyranoside—11.7—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F283LBacillus sp.3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor glucose); cosubstrate glucose7.30.1549 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F283LBacillus sp.3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor maltose); cosubstrate maltose8.20.1268 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F283LBacillus sp.alpha-cyclodextrin0.230.940.24 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F283LBacillus sp.amylose (average degree of polymerization of 17)120.0724170 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F283LBacillus sp.D-glucose—7.3—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F283LBacillus sp.gamma-cyclodextrin2.10.121 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F283LBacillus sp.maltose—0.17—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant F283LBacillus sp.methyl alpha-D-glucopyranoside—39.9—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant Y195FBacillus sp.3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor glucose); cosubstrate glucose1390.2700 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant Y195FBacillus sp.3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor maltose); cosubstrate maltose1920.22870 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant Y195FBacillus sp.alpha-cyclodextrin1.530.552.8 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant Y195FBacillus sp.amylose (average degree of polymerization of 17)28.90.0556520 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant Y195FBacillus sp.D-glucose—15.9—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant Y195FBacillus sp.gamma-cyclodextrin29.20.1290 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant Y195FBacillus sp.maltose—0.59—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant Y195FBacillus sp.methyl alpha-D-glucopyranoside—17.4—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant Y195LBacillus sp.3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor glucose); cosubstrate glucose1180.34350 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant Y195LBacillus sp.3-ketobutylidene-β-2-chloro-4-nitrophenylmaltopentaoside(donor 3KB-G5CNP, acceptor maltose); cosubstrate maltose1320.31430 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant Y195LBacillus sp.alpha-cyclodextrin0.112.560.043 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant Y195LBacillus sp.amylose (average degree of polymerization of 17)8.90.0618140 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant Y195LBacillus sp.beta-cyclodextrin0.840.441.9 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant Y195LBacillus sp.D-glucose—9.8—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant Y195LBacillus sp.gamma-cyclodextrin18.90.5137 calc.pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant Y195LBacillus sp.maltose—0.38—pH 6.5, 37 °COpen
cyclodextrin glucanotransferase (CGTase)variant Y195LBacillus sp.methyl alpha-D-glucopyranoside—12—pH 6.5, 37 °COpen