Values CatLog read from this paper
Probing the conformational change of Escherichia coli undecaprenyl pyrophosphate synthase during catalysis using an inhibitor and tryptophan mutants
PMID 11744728doi:10.1074/jbc.m110014200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 15 records (22 values) from this paper: undecaprenyl pyrophosphate synthase (UPPS) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| undecaprenyl pyrophosphate synthase (UPPS) | Escherichia coli | (S)-farnesyl thiopyrophosphate | 3.1 × 10⁻⁷ | — | — | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPS), (S)-farnesyl thiopyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPS) | Escherichia coli | all-trans-farnesyl pyrophosphate | 2.5 | 4 × 10⁻⁴ | 6.2 × 10³ calc. | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPS), all-trans-farnesyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPS) | Escherichia coli | isopentenyl pyrophosphate | — | 0.0041 | — | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPS), isopentenyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPS)variant W149F | Escherichia coli | all-trans-farnesyl pyrophosphate | 1.7 | 7 × 10⁻⁴ | 2.4 × 10³ calc. | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPS) variant W149F, all-trans-farnesyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPS)variant W149F | Escherichia coli | isopentenyl pyrophosphate | — | 0.02 | — | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPS) variant W149F, isopentenyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPS)variant W207F | Escherichia coli | all-trans-farnesyl pyrophosphate | 1.5 | 0.0034 | 440 calc. | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPS) variant W207F, all-trans-farnesyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPS)variant W207F | Escherichia coli | isopentenyl pyrophosphate | — | 0.023 | — | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPS) variant W207F, isopentenyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPS)variant W221F | Escherichia coli | all-trans-farnesyl pyrophosphate | 3.5 | 5 × 10⁻⁴ | 7.0 × 10³ calc. | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPS) variant W221F, all-trans-farnesyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPS)variant W221F | Escherichia coli | isopentenyl pyrophosphate | — | 0.014 | — | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPS) variant W221F, isopentenyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPS)variant W31F | Escherichia coli | all-trans-farnesyl pyrophosphate | 1.1 | 0.002 | 550 calc. | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPS) variant W31F, all-trans-farnesyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPS)variant W31F | Escherichia coli | isopentenyl pyrophosphate | — | 0.0059 | — | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPS) variant W31F, isopentenyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPS)variant W75F | Escherichia coli | all-trans-farnesyl pyrophosphate | 1.1 | 0.0018 | 610 calc. | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPS) variant W75F, all-trans-farnesyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPS)variant W75F | Escherichia coli | isopentenyl pyrophosphate | — | 0.026 | — | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPS) variant W75F, isopentenyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPS)variant W91F | Escherichia coli | all-trans-farnesyl pyrophosphate | 2.5 | 4 × 10⁻⁴ | 6.2 × 10³ calc. | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPS) variant W91F, all-trans-farnesyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPS)variant W91F | Escherichia coli | isopentenyl pyrophosphate | — | 0.0072 | — | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPS) variant W91F, isopentenyl pyrophosphate |