Values CatLog read from this paper

Determinants of L-aspartate and IMP recognition in Escherichia coli adenylosuccinate synthetase

PMID 11781326doi:10.1074/jbc.m111810200Open in Browse

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CatLog read 36 records (69 values) from this paper: adenylosuccinate synthetase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.3.4.4

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
adenylosuccinate synthetase	Escherichia coli	guanosine 5'-triphosphate	—	0.19	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase, guanosine 5'-triphosphate
adenylosuccinate synthetase	Escherichia coli	hydroxylamine	0.5	230	0.0022 calc.	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase, hydroxylamine
adenylosuccinate synthetase	Escherichia coli	inosine 5'-monophosphate	—	0.066	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase, inosine 5'-monophosphate
adenylosuccinate synthetasevariant T128A	Escherichia coli	guanosine 5'-triphosphate	—	0.054	9	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T128A, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant T128A	Escherichia coli	inosine 5'-monophosphate	—	0.31	1.5	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T128A, inosine 5'-monophosphate
adenylosuccinate synthetasevariant T128A	Escherichia coli	L-aspartate	0.46	0.17	2.7	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T128A, L-aspartate
adenylosuccinate synthetasevariant T129A	Escherichia coli	guanosine 5'-triphosphate	—	1.7	0.25	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T129A, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant T129A	Escherichia coli	inosine 5'-monophosphate	—	0.89	0.47	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T129A, inosine 5'-monophosphate
adenylosuccinate synthetasevariant T129A	Escherichia coli	L-aspartate	0.42	0.24	1.75	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T129A, L-aspartate
adenylosuccinate synthetasevariant T300A	Escherichia coli	guanosine 5'-triphosphate	—	0.17	3.9	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T300A, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant T300A	Escherichia coli	inosine 5'-monophosphate	—	0.019	34	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T300A, inosine 5'-monophosphate
adenylosuccinate synthetasevariant T300A	Escherichia coli	L-aspartate	0.64	6.2	0.1	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T300A, L-aspartate
adenylosuccinate synthetasevariant T300V	Escherichia coli	guanosine 5'-triphosphate	—	0.056	60	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T300V, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant T300V	Escherichia coli	inosine 5'-monophosphate	—	0.033	100	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T300V, inosine 5'-monophosphate
adenylosuccinate synthetasevariant T300V	Escherichia coli	L-aspartate	3.3	0.82	4.1	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T300V, L-aspartate
adenylosuccinate synthetasevariant T301A	Escherichia coli	guanosine 5'-triphosphate	—	0.057	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T301A, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant T301A	Escherichia coli	hydroxylamine	0.24	91	0.0026 calc.	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T301A, hydroxylamine
adenylosuccinate synthetasevariant T301A	Escherichia coli	inosine 5'-monophosphate	—	0.32	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T301A, inosine 5'-monophosphate
adenylosuccinate synthetasevariant V273A	Escherichia coli	guanosine 5'-triphosphateL-aspartate reaction: 20 mM Hepes pH 7.7	—	0.031	32	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273A, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant V273A	Escherichia coli	guanosine 5'-triphosphatemagnesium chloride; UV difference assay at 280 nm	—	0.14	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273A, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant V273A	Escherichia coli	hydroxylamine	0.21	255	8.2 × 10⁻⁴ calc.	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273A, hydroxylamine
adenylosuccinate synthetasevariant V273A	Escherichia coli	inosine 5'-monophosphateL-aspartate reaction: 20 mM Hepes pH 7.7	—	0.017	59	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273A, inosine 5'-monophosphate
adenylosuccinate synthetasevariant V273A	Escherichia coli	inosine 5'-monophosphatemagnesium chloride; UV difference assay at 280 nm	—	0.14	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273A, inosine 5'-monophosphate
adenylosuccinate synthetasevariant V273A	Escherichia coli	L-aspartate	1	7	0.14	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273A, L-aspartate
adenylosuccinate synthetasevariant V273N	Escherichia coli	guanosine 5'-triphosphateL-aspartate reaction: 20 mM Hepes pH 7.7	—	0.021	2.4	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273N, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant V273N	Escherichia coli	guanosine 5'-triphosphatemagnesium chloride; UV difference assay at 280 nm	—	0.18	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273N, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant V273N	Escherichia coli	hydroxylamine	0.29	110	0.0026 calc.	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273N, hydroxylamine
adenylosuccinate synthetasevariant V273N	Escherichia coli	inosine 5'-monophosphateL-aspartate reaction: 20 mM Hepes pH 7.7	—	0.024	2.1	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273N, inosine 5'-monophosphate
adenylosuccinate synthetasevariant V273N	Escherichia coli	inosine 5'-monophosphatemagnesium chloride; UV difference assay at 280 nm	—	0.113	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273N, inosine 5'-monophosphate
adenylosuccinate synthetasevariant V273N	Escherichia coli	L-aspartate	0.05	9	0.006	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273N, L-aspartate
adenylosuccinate synthetasevariant V273T	Escherichia coli	guanosine 5'-triphosphateL-aspartate reaction: 20 mM Hepes pH 7.7	—	0.045	44	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273T, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant V273T	Escherichia coli	guanosine 5'-triphosphatemagnesium chloride; UV difference assay at 280 nm	—	0.074	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273T, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant V273T	Escherichia coli	hydroxylamine	0.08	140	5.7 × 10⁻⁴ calc.	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273T, hydroxylamine
adenylosuccinate synthetasevariant V273T	Escherichia coli	inosine 5'-monophosphateL-aspartate reaction: 20 mM Hepes pH 7.7	—	0.043	46	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273T, inosine 5'-monophosphate
adenylosuccinate synthetasevariant V273T	Escherichia coli	inosine 5'-monophosphatemagnesium chloride; UV difference assay at 280 nm	—	0.077	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273T, inosine 5'-monophosphate
adenylosuccinate synthetasevariant V273T	Escherichia coli	L-aspartate	1.99	3.4	0.61	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant V273T, L-aspartate