Values CatLog read from this paper
Active site structure and stereospecificity of Escherichia coli pyridoxine-5'-phosphate oxidase
PMID 11786019doi:10.1006/jmbi.2001.5254Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (18 values) from this paper: pyridoxine 5'-phosphate oxidase (PNPOx) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 9 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| pyridoxine 5'-phosphate oxidase (PNPOx) | Escherichia coli | pyridoxine 5'-phosphate | 0.13 | 3 × 10⁻⁴ | 430 calc. | pH 8.5 | Open record: pyridoxine 5'-phosphate oxidase (PNPOx), pyridoxine 5'-phosphate |
| pyridoxine 5'-phosphate oxidase (PNPOx)variant D49A | Escherichia coli | pyridoxine 5'-phosphate | 0.06 | 0.001 | 60 calc. | pH 8.5 | Open record: pyridoxine 5'-phosphate oxidase (PNPOx) variant D49A, pyridoxine 5'-phosphate |
| pyridoxine 5'-phosphate oxidase (PNPOx)variant H199A | Escherichia coli | pyridoxine 5'-phosphate | 0.14 | 0.07 | 2.0 calc. | pH 8.5 | Open record: pyridoxine 5'-phosphate oxidase (PNPOx) variant H199A, pyridoxine 5'-phosphate |
| pyridoxine 5'-phosphate oxidase (PNPOx)variant H199N | Escherichia coli | pyridoxine 5'-phosphate | 0.03 | 3 × 10⁻⁴ | 100 calc. | pH 8.5 | Open record: pyridoxine 5'-phosphate oxidase (PNPOx) variant H199N, pyridoxine 5'-phosphate |
| pyridoxine 5'-phosphate oxidase (PNPOx)variant R14E | Escherichia coli | pyridoxine 5'-phosphate | 0.16 | 0.002 | 80 calc. | pH 8.5 | Open record: pyridoxine 5'-phosphate oxidase (PNPOx) variant R14E, pyridoxine 5'-phosphate |
| pyridoxine 5'-phosphate oxidase (PNPOx)variant R14M | Escherichia coli | pyridoxine 5'-phosphate | 0.14 | 0.0026 | 54 calc. | pH 8.5 | Open record: pyridoxine 5'-phosphate oxidase (PNPOx) variant R14M, pyridoxine 5'-phosphate |
| pyridoxine 5'-phosphate oxidase (PNPOx)variant R197E | Escherichia coli | pyridoxine 5'-phosphate | 0.008 | 2.4 | 0.0033 calc. | pH 8.5 | Open record: pyridoxine 5'-phosphate oxidase (PNPOx) variant R197E, pyridoxine 5'-phosphate |
| pyridoxine 5'-phosphate oxidase (PNPOx)variant R197M | Escherichia coli | pyridoxine 5'-phosphate | 0.03 | 0.09 | 0.33 calc. | pH 8.5 | Open record: pyridoxine 5'-phosphate oxidase (PNPOx) variant R197M, pyridoxine 5'-phosphate |
| pyridoxine 5'-phosphate oxidase (PNPOx)variant Y17F | Escherichia coli | pyridoxine 5'-phosphate | 0.6 | 0.001 | 600 calc. | pH 8.5 | Open record: pyridoxine 5'-phosphate oxidase (PNPOx) variant Y17F, pyridoxine 5'-phosphate |