CatLogenzyme kinetics catalog

EC 1.4.3.5: pyridoxal 5'-phosphate synthase

OxidoreductasesOpen in Browse

CatLog read 32 records for EC 1.4.3.5 from 4 papers, for 3 organisms. Names in these papers: pyridoxine 5'-phosphate oxidase (ePNPO), pyridoxine 5'-phosphate oxidase (PNPOx), pyridoxine 5'-phosphate oxidase (PNPO), pyridoxine 5'-phosphate oxidase PdxH3 (ScPdxH).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 45 records for EC 1.4.3.5 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.4.3.5 in Browse.

Papers

PaperOrganismsRecords
Identification and characterization of the pyridoxal 5'-phosphate allosteric site in Escherichia coli pyridoxine 5'-phosphate oxidaseEscherichia coli12
Active site structure and stereospecificity of Escherichia coli pyridoxine-5'-phosphate oxidaseEscherichia coli9
Molecular characterization of pyridoxine 5'-phosphate oxidase and its pathogenic forms associated with neonatal epileptic encephalopathyHomo sapiens7
Two-step bioconversion of pyridoxal 5'-phosphate from pyridoxine through cofactor regeneration and pyridoxine 5'-phosphate oxidase evolutionSaccharomyces cerevisiae4

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
pyridoxine 5'-phosphate oxidase (ePNPO)Escherichia colipyridoxine 5'-phosphate0.250.0016160 calc.pH 7.6, 37 °CPMID 34019876Open
pyridoxine 5'-phosphate oxidase (ePNPO)variant K145A/F177AEscherichia colipyridoxine 5'-phosphate0.170.002471 calc.pH 7.6, 37 °CPMID 34019876Open
pyridoxine 5'-phosphate oxidase (ePNPO)variant N84A/K145A/F177AEscherichia colipyridoxine 5'-phosphate0.070.003222 calc.pH 7.6, 37 °CPMID 34019876Open
pyridoxine 5'-phosphate oxidase (ePNPO)variant N84W/K145A/F177AEscherichia colipyridoxine 5'-phosphate0.050.003116 calc.pH 7.6, 37 °CPMID 34019876Open
pyridoxine 5'-phosphate oxidase (ePNPO)variant R215LEscherichia colipyridoxine 5'-phosphate0.050.002619 calc.pH 7.6, 37 °CPMID 34019876Open
pyridoxine 5'-phosphate oxidase (ePNPO)variant R23LEscherichia colipyridoxine 5'-phosphate0.090.001753 calc.pH 7.6, 37 °CPMID 34019876Open
pyridoxine 5'-phosphate oxidase (ePNPO)variant R23L/R215LEscherichia colipyridoxine 5'-phosphate50 mM Tris-HCl buffer; saturating FMN (up to 3-6 µM)0.050.003216 calc.pH 7.6, 37 °CPMID 34019876Open
pyridoxine 5'-phosphate oxidase (ePNPO)variant R23L/R215LEscherichia colipyridoxine 5'-phosphate50 mM Na-Hepes buffer; PLP formation followed at 388 nm—0.0054—pH 7.6, 37 °CPMID 34019876Open
pyridoxine 5'-phosphate oxidase (ePNPO)variant R23L/R24LEscherichia colipyridoxine 5'-phosphate50 mM Tris-HCl buffer; saturating FMN (up to 3-6 µM)0.190.002190 calc.pH 7.6, 37 °CPMID 34019876Open
pyridoxine 5'-phosphate oxidase (ePNPO)variant R23L/R24LEscherichia colipyridoxine 5'-phosphate50 mM Na-Hepes buffer; PLP formation followed at 388 nm—8 × 10⁻⁴—pH 7.6, 37 °CPMID 34019876Open
pyridoxine 5'-phosphate oxidase (ePNPO)variant R23L/R24L/R215LEscherichia colipyridoxine 5'-phosphate50 mM Tris-HCl buffer; saturating FMN (up to 3-6 µM)0.090.003426 calc.pH 7.6, 37 °CPMID 34019876Open
pyridoxine 5'-phosphate oxidase (ePNPO)variant R23L/R24L/R215LEscherichia colipyridoxine 5'-phosphate50 mM Na-Hepes buffer; PLP formation followed at 388 nm—0.00319—pH 7.6, 37 °CPMID 34019876Open
pyridoxine 5'-phosphate oxidase (PNPO)Homo sapienspyridoxine 5'-phosphate50 mM Tris-HCl pH 7.6; PLP-Tris aldimine followed at 414 nm0.0620.002624 calc.pH 7.6, 37 °CPMID 32788630Open
pyridoxine 5'-phosphate oxidase (PNPO)Homo sapienspyridoxine 5'-phosphate50 mM Na-HEPES pH 7.6; free PLP followed at 388 nm; Table 10.0570.002820 calc.pH 7.6, 37 °CPMID 32788630Open
pyridoxine 5'-phosphate oxidase (PNPO)Homo sapienspyridoxine 5'-phosphate50 mM Na-HEPES pH 7.6; free PLP followed at 388 nm—0.00197—pH 7.6, 37 °CPMID 32788630Open
pyridoxine 5'-phosphate oxidase (PNPO)variant G118RHomo sapienspyridoxine 5'-phosphate0.0220.00573.9 calc.pH 7.6, 37 °CPMID 32788630Open
pyridoxine 5'-phosphate oxidase (PNPO)variant R116Q/R225HHomo sapienspyridoxine 5'-phosphate0.0120.00512.4 calc.pH 7.6, 37 °CPMID 32788630Open
pyridoxine 5'-phosphate oxidase (PNPO)variant R141CHomo sapienspyridoxine 5'-phosphate0.0190.00613.1 calc.pH 7.6, 37 °CPMID 32788630Open
pyridoxine 5'-phosphate oxidase (PNPO)variant R225HHomo sapienspyridoxine 5'-phosphate0.0020.0160.12 calc.pH 7.6, 37 °CPMID 32788630Open
pyridoxine 5'-phosphate oxidase (PNPOx)Escherichia colipyridoxine 5'-phosphate0.133 × 10⁻⁴430 calc.pH 8.5PMID 11786019Open
pyridoxine 5'-phosphate oxidase (PNPOx)variant D49AEscherichia colipyridoxine 5'-phosphate0.060.00160 calc.pH 8.5PMID 11786019Open
pyridoxine 5'-phosphate oxidase (PNPOx)variant H199AEscherichia colipyridoxine 5'-phosphate0.140.072.0 calc.pH 8.5PMID 11786019Open
pyridoxine 5'-phosphate oxidase (PNPOx)variant H199NEscherichia colipyridoxine 5'-phosphate0.033 × 10⁻⁴100 calc.pH 8.5PMID 11786019Open
pyridoxine 5'-phosphate oxidase (PNPOx)variant R14EEscherichia colipyridoxine 5'-phosphate0.160.00280 calc.pH 8.5PMID 11786019Open
pyridoxine 5'-phosphate oxidase (PNPOx)variant R14MEscherichia colipyridoxine 5'-phosphate0.140.002654 calc.pH 8.5PMID 11786019Open
pyridoxine 5'-phosphate oxidase (PNPOx)variant R197EEscherichia colipyridoxine 5'-phosphate0.0082.40.0033 calc.pH 8.5PMID 11786019Open
pyridoxine 5'-phosphate oxidase (PNPOx)variant R197MEscherichia colipyridoxine 5'-phosphate0.030.090.33 calc.pH 8.5PMID 11786019Open
pyridoxine 5'-phosphate oxidase (PNPOx)variant Y17FEscherichia colipyridoxine 5'-phosphate0.60.001600 calc.pH 8.5PMID 11786019Open
pyridoxine 5'-phosphate oxidase PdxH3 (ScPdxH)Saccharomyces cerevisiaepyridoxine 5'-phosphate0.01080.320.0338pH 7.6, 30 °CPMID 42006857Open
pyridoxine 5'-phosphate oxidase PdxH3 (ScPdxH)variant 8-5HSaccharomyces cerevisiaepyridoxine 5'-phosphate0.01550.20.0775pH 7.6, 30 °CPMID 42006857Open
pyridoxine 5'-phosphate oxidase PdxH3 (ScPdxH)variant 8-6HSaccharomyces cerevisiaepyridoxine 5'-phosphate0.0140.270.0518pH 7.6, 30 °CPMID 42006857Open
pyridoxine 5'-phosphate oxidase PdxH3 (ScPdxH)variant E122V/L206S/K222Q (2-3E)Saccharomyces cerevisiaepyridoxine 5'-phosphate0.01620.140.1155pH 7.6, 30 °CPMID 42006857Open