CatLogenzyme kinetics catalog

Values CatLog read from this paper

Identification and characterization of the pyridoxal 5'-phosphate allosteric site in Escherichia coli pyridoxine 5'-phosphate oxidase

PMID 34019876doi:10.1016/j.jbc.2021.100795Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 12 records (21 values) from this paper: pyridoxine 5'-phosphate oxidase (ePNPO) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 9 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.4.3.5

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
pyridoxine 5'-phosphate oxidase (ePNPO)Escherichia colipyridoxine 5'-phosphate0.250.0016160 calc.pH 7.6, 37 °COpen
pyridoxine 5'-phosphate oxidase (ePNPO)variant K145A/F177AEscherichia colipyridoxine 5'-phosphate0.170.002471 calc.pH 7.6, 37 °COpen
pyridoxine 5'-phosphate oxidase (ePNPO)variant N84A/K145A/F177AEscherichia colipyridoxine 5'-phosphate0.070.003222 calc.pH 7.6, 37 °COpen
pyridoxine 5'-phosphate oxidase (ePNPO)variant N84W/K145A/F177AEscherichia colipyridoxine 5'-phosphate0.050.003116 calc.pH 7.6, 37 °COpen
pyridoxine 5'-phosphate oxidase (ePNPO)variant R215LEscherichia colipyridoxine 5'-phosphate0.050.002619 calc.pH 7.6, 37 °COpen
pyridoxine 5'-phosphate oxidase (ePNPO)variant R23LEscherichia colipyridoxine 5'-phosphate0.090.001753 calc.pH 7.6, 37 °COpen
pyridoxine 5'-phosphate oxidase (ePNPO)variant R23L/R215LEscherichia colipyridoxine 5'-phosphate50 mM Tris-HCl buffer; saturating FMN (up to 3-6 µM)0.050.003216 calc.pH 7.6, 37 °COpen
pyridoxine 5'-phosphate oxidase (ePNPO)variant R23L/R215LEscherichia colipyridoxine 5'-phosphate50 mM Na-Hepes buffer; PLP formation followed at 388 nm—0.0054—pH 7.6, 37 °COpen
pyridoxine 5'-phosphate oxidase (ePNPO)variant R23L/R24LEscherichia colipyridoxine 5'-phosphate50 mM Tris-HCl buffer; saturating FMN (up to 3-6 µM)0.190.002190 calc.pH 7.6, 37 °COpen
pyridoxine 5'-phosphate oxidase (ePNPO)variant R23L/R24LEscherichia colipyridoxine 5'-phosphate50 mM Na-Hepes buffer; PLP formation followed at 388 nm—8 × 10⁻⁴—pH 7.6, 37 °COpen
pyridoxine 5'-phosphate oxidase (ePNPO)variant R23L/R24L/R215LEscherichia colipyridoxine 5'-phosphate50 mM Tris-HCl buffer; saturating FMN (up to 3-6 µM)0.090.003426 calc.pH 7.6, 37 °COpen
pyridoxine 5'-phosphate oxidase (ePNPO)variant R23L/R24L/R215LEscherichia colipyridoxine 5'-phosphate50 mM Na-Hepes buffer; PLP formation followed at 388 nm—0.00319—pH 7.6, 37 °COpen