CatLogenzyme kinetics catalog

Values CatLog read from this paper

Molecular characterization of pyridoxine 5'-phosphate oxidase and its pathogenic forms associated with neonatal epileptic encephalopathy

PMID 32788630doi:10.1038/s41598-020-70598-7Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 7 records (13 values) from this paper: pyridoxine 5'-phosphate oxidase (PNPO) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.4.3.5

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
pyridoxine 5'-phosphate oxidase (PNPO)Homo sapienspyridoxine 5'-phosphate50 mM Tris-HCl pH 7.6; PLP-Tris aldimine followed at 414 nm0.0620.002624 calc.pH 7.6, 37 °COpen
pyridoxine 5'-phosphate oxidase (PNPO)Homo sapienspyridoxine 5'-phosphate50 mM Na-HEPES pH 7.6; free PLP followed at 388 nm; Table 10.0570.002820 calc.pH 7.6, 37 °COpen
pyridoxine 5'-phosphate oxidase (PNPO)Homo sapienspyridoxine 5'-phosphate50 mM Na-HEPES pH 7.6; free PLP followed at 388 nm—0.00197—pH 7.6, 37 °COpen
pyridoxine 5'-phosphate oxidase (PNPO)variant G118RHomo sapienspyridoxine 5'-phosphate0.0220.00573.9 calc.pH 7.6, 37 °COpen
pyridoxine 5'-phosphate oxidase (PNPO)variant R116Q/R225HHomo sapienspyridoxine 5'-phosphate0.0120.00512.4 calc.pH 7.6, 37 °COpen
pyridoxine 5'-phosphate oxidase (PNPO)variant R141CHomo sapienspyridoxine 5'-phosphate0.0190.00613.1 calc.pH 7.6, 37 °COpen
pyridoxine 5'-phosphate oxidase (PNPO)variant R225HHomo sapienspyridoxine 5'-phosphate0.0020.0160.12 calc.pH 7.6, 37 °COpen