CatLogenzyme kinetics catalog

Values CatLog read from this paper

The streptococcal hyaluronan synthases are inhibited by sulfhydryl-modifying reagents, but conserved cysteine residues are not essential for enzyme function

PMID 11799120doi:10.1074/jbc.m110638200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 38 records (38 values) from this paper: hyaluronan synthase (seHAS) from Streptococcus equisimilis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.4.1.212

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-alpha-D-glucuronic acidPBS alone (control)—0.085—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-alpha-D-glucuronic acid5 mM N-ethylmaleimide (NEM) in PBS—0.057—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-alpha-D-glucuronic acid10 mM sodium arsenite in PBS—0.067—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-alpha-D-glucuronic acid—0.077—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetyl-alpha-D-glucosaminePBS alone (control)—0.122—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine5 mM N-ethylmaleimide (NEM) in PBS—0.082—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine10 mM sodium arsenite in PBS—0.112—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.074—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C226AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.088—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C226AStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.154—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C226A/C262AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.134—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C226A/C262A/C281AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.12—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C226A/C262A/C281A/C367AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.21—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C226A/C262A/C367AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.109—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C226A/C281AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.053—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C226A/C281AStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.108—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C226A/C281A/C367AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.087—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C226A/C367AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.079—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C226A/C367AStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.149—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C226SStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.044—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C226SStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.232—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C262AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.146—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C262AStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.186—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C262A/C281AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.113—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C262A/C281A/C367AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.081—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C262A/C367AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.121—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C262SStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.096—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C262SStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.153—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C281AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.04—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C281AStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.13—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C281A/C367AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.065—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C281A/C367AStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.131—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C281SStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.056—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C281SStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.098—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C367AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.085—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C367AStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.09—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C367SStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.079—pH 7, 30 °COpen
hyaluronan synthase (seHAS)variant C367SStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.091—pH 7, 30 °COpen