CatLogenzyme kinetics catalog

EC 2.4.1.212: hyaluronan synthase

TransferasesOpen in Browse

CatLog read 110 records for EC 2.4.1.212 from 4 papers, for 3 organisms. Names in these papers: hyaluronan synthase (seHAS), hyaluronan synthase (spHAS), hyaluronan synthase 1 (HAS1), hyaluronan synthase 2 (HAS2) and 1 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 36 records for EC 2.4.1.212 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.4.1.212 in Browse.

Papers

PaperOrganismsRecords
The streptococcal hyaluronan synthases are inhibited by sulfhydryl-modifying reagents, but conserved cysteine residues are not essential for enzyme functionStreptococcus equisimilis38
Kinetic characterization of the recombinant hyaluronan synthases from Streptococcus pyogenes and Streptococcus equisimilisStreptococcus equisimilis, Streptococcus pyogenes36
Three isoforms of mammalian hyaluronan synthases have distinct enzymatic propertiesMus musculus18
Site-directed mutation of conserved cysteine residues does not inactivate the Streptococcus pyogenes hyaluronan synthaseStreptococcus pyogenes18

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-alpha-D-glucuronic acidPBS alone (control)—0.085—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-alpha-D-glucuronic acid5 mM N-ethylmaleimide (NEM) in PBS—0.057—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-alpha-D-glucuronic acid10 mM sodium arsenite in PBS—0.067—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-alpha-D-glucuronic acid—0.077—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 0.01 mM; in E. coli membranes—0.005—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 0.05 mM; in E. coli membranes—0.017—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 0.1 mM; in E. coli membranes—0.02—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 0.5 mM; in E. coli membranes—0.051—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 1.0 mM; in E. coli membranes—0.076—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 0.05 mM; 2 mM bovine cardiolipin—0.022—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 0.1 mM; 2 mM bovine cardiolipin—0.04—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 0.5 mM; 2 mM bovine cardiolipin—0.09—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 1.0 mM; 2 mM bovine cardiolipin—0.163—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 1.5 mM; 2 mM bovine cardiolipin—0.274—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 2.0 mM; 2 mM bovine cardiolipin—0.37—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidno added cardiolipin; 0.98 mM n-dodecyl beta-D-maltoside—0.054—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetyl-alpha-D-glucosaminePBS alone (control)—0.122—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine5 mM N-ethylmaleimide (NEM) in PBS—0.082—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine10 mM sodium arsenite in PBS—0.112—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.074—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 0.01 mM; in E. coli membranes—0.009—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 0.05 mM; in E. coli membranes—0.019—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 0.1 mM; in E. coli membranes—0.03—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 0.5 mM; in E. coli membranes—0.06—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 1.0 mM; in E. coli membranes—0.074—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 0.01 mM; 2 mM bovine cardiolipin—0.018—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 0.05 mM; 2 mM bovine cardiolipin—0.041—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 0.1 mM; 2 mM bovine cardiolipin—0.062—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 0.5 mM; 2 mM bovine cardiolipin—0.164—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 1.0 mM; 2 mM bovine cardiolipin—0.251—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 1.5 mM; 2 mM bovine cardiolipin—0.329—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineno added cardiolipin; 0.98 mM n-dodecyl beta-D-maltoside—1.06—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (seHAS)variant C226AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.088—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C226AStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.154—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C226A/C262AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.134—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C226A/C262A/C281AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.12—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C226A/C262A/C281A/C367AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.21—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C226A/C262A/C367AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.109—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C226A/C281AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.053—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C226A/C281AStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.108—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C226A/C281A/C367AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.087—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C226A/C367AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.079—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C226A/C367AStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.149—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C226SStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.044—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C226SStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.232—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C262AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.146—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C262AStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.186—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C262A/C281AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.113—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C262A/C281A/C367AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.081—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C262A/C367AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.121—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C262SStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.096—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C262SStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.153—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C281AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.04—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C281AStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.13—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C281A/C367AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.065—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C281A/C367AStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.131—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C281SStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.056—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C281SStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.098—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C367AStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.085—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C367AStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.09—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C367SStreptococcus equisimilisUDP-alpha-D-glucuronic acid—0.079—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (seHAS)variant C367SStreptococcus equisimilisUDP-N-acetyl-alpha-D-glucosamine—0.091—pH 7, 30 °CPMID 11799120Open
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-GlcUA—0.0504—PMID 11805074Open
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 0.1 mM; in E. coli membranes—0.017—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 0.2 mM; in E. coli membranes—0.025—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 0.5 mM; in E. coli membranes—0.027—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 1.0 mM; in E. coli membranes—0.035—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 1.5 mM; in E. coli membranes—0.04—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 0.1 mM; 2 mM bovine cardiolipin—0.02—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 0.2 mM; 2 mM bovine cardiolipin—0.036—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 0.5 mM; 2 mM bovine cardiolipin—0.066—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 1.0 mM; 2 mM bovine cardiolipin—0.131—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 1.5 mM; 2 mM bovine cardiolipin—0.175—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 2.0 mM; 2 mM bovine cardiolipin—0.216—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidno added cardiolipin; 0.98 mM n-dodecyl beta-D-maltoside—0.062—pH 7, 30 °CPMID 9933624Open
hyaluronan synthase (spHAS)variant C124AStreptococcus pyogenesUDP-GlcUA—0.0373—PMID 11805074Open
hyaluronan synthase (spHAS)variant C124A/C261A/C280A/C366A/C402AStreptococcus pyogenesUDP-GlcUA—0.101—PMID 11805074Open
hyaluronan synthase (spHAS)variant C124A/C366A/C402AStreptococcus pyogenesUDP-GlcUA—0.13—PMID 11805074Open
hyaluronan synthase (spHAS)variant C124A/C402AStreptococcus pyogenesUDP-GlcUA—0.0395—PMID 11805074Open
hyaluronan synthase (spHAS)variant C124SStreptococcus pyogenesUDP-GlcUA—0.0327—PMID 11805074Open
hyaluronan synthase (spHAS)variant C225AStreptococcus pyogenesUDP-GlcUA—0.0622—PMID 11805074Open
hyaluronan synthase (spHAS)variant C225SStreptococcus pyogenesUDP-GlcUA—0.0427—PMID 11805074Open
hyaluronan synthase (spHAS)variant C261AStreptococcus pyogenesUDP-GlcUA—0.0426—PMID 11805074Open
hyaluronan synthase (spHAS)variant C261A/C280AStreptococcus pyogenesUDP-GlcUA—0.0548—PMID 11805074Open
hyaluronan synthase (spHAS)variant C261SStreptococcus pyogenesUDP-GlcUA—0.0456—PMID 11805074Open
hyaluronan synthase (spHAS)variant C280AStreptococcus pyogenesUDP-GlcUA—0.0338—PMID 11805074Open
hyaluronan synthase (spHAS)variant C280SStreptococcus pyogenesUDP-GlcUA—0.0294—PMID 11805074Open
hyaluronan synthase (spHAS)variant C366AStreptococcus pyogenesUDP-GlcUA—0.0568—PMID 11805074Open
hyaluronan synthase (spHAS)variant C366SStreptococcus pyogenesUDP-GlcUA—0.0341—PMID 11805074Open
hyaluronan synthase (spHAS)variant C402AStreptococcus pyogenesUDP-GlcUA—0.0438—PMID 11805074Open
hyaluronan synthase (spHAS)variant C402SStreptococcus pyogenesUDP-GlcUA—0.0444—PMID 11805074Open
hyaluronan synthase (spHAS)variant Cys-null (C124A/C225A/C261A/C280A/C366A/C402A)Streptococcus pyogenesUDP-GlcUA—0.153—PMID 11805074Open
hyaluronan synthase 1 (HAS1)Mus musculusUDP-alpha-D-glucuronic acidfixed UDP-GlcNAc at 0.1 mM; cosubstrate UDP-GlcNAc (0.1 mM)—0.0732—pH 7.1, 37 °CPMID 10455188Open
hyaluronan synthase 1 (HAS1)Mus musculusUDP-alpha-D-glucuronic acidfixed UDP-GlcNAc at 0.5 mM; cosubstrate UDP-GlcNAc (0.5 mM)—0.0533—pH 7.1, 37 °CPMID 10455188Open
hyaluronan synthase 1 (HAS1)Mus musculusUDP-alpha-D-glucuronic acidfixed UDP-GlcNAc at 2.0 mM; cosubstrate UDP-GlcNAc (2.0 mM)—0.0729—pH 7.1, 37 °CPMID 10455188Open
hyaluronan synthase 1 (HAS1)Mus musculusUDP-N-acetyl-alpha-D-glucosaminefixed UDP-GlcUA at 0.05 mM; cosubstrate UDP-GlcUA (0.05 mM)—0.799—pH 7.1, 37 °CPMID 10455188Open
hyaluronan synthase 1 (HAS1)Mus musculusUDP-N-acetyl-alpha-D-glucosaminefixed UDP-GlcUA at 0.2 mM; cosubstrate UDP-GlcUA (0.2 mM)—0.5009—pH 7.1, 37 °CPMID 10455188Open
hyaluronan synthase 1 (HAS1)Mus musculusUDP-N-acetyl-alpha-D-glucosaminefixed UDP-GlcUA at 1.0 mM; cosubstrate UDP-GlcUA (1.0 mM)—1.011—pH 7.1, 37 °CPMID 10455188Open
hyaluronan synthase 2 (HAS2)Mus musculusUDP-alpha-D-glucuronic acidfixed UDP-GlcNAc at 0.1 mM; cosubstrate UDP-GlcNAc (0.1 mM)—0.0329—pH 7.1, 37 °CPMID 10455188Open
hyaluronan synthase 2 (HAS2)Mus musculusUDP-alpha-D-glucuronic acidfixed UDP-GlcNAc at 0.5 mM; cosubstrate UDP-GlcNAc (0.5 mM)—0.0292—pH 7.1, 37 °CPMID 10455188Open
hyaluronan synthase 2 (HAS2)Mus musculusUDP-alpha-D-glucuronic acidfixed UDP-GlcNAc at 2.0 mM; cosubstrate UDP-GlcNAc (2.0 mM)—0.03—pH 7.1, 37 °CPMID 10455188Open
hyaluronan synthase 2 (HAS2)Mus musculusUDP-N-acetyl-alpha-D-glucosaminefixed UDP-GlcUA at 0.05 mM; cosubstrate UDP-GlcUA (0.05 mM)—0.1076—pH 7.1, 37 °CPMID 10455188Open
hyaluronan synthase 2 (HAS2)Mus musculusUDP-N-acetyl-alpha-D-glucosaminefixed UDP-GlcUA at 0.2 mM; cosubstrate UDP-GlcUA (0.2 mM)—0.3306—pH 7.1, 37 °CPMID 10455188Open
hyaluronan synthase 2 (HAS2)Mus musculusUDP-N-acetyl-alpha-D-glucosaminefixed UDP-GlcUA at 1.0 mM; cosubstrate UDP-GlcUA (1.0 mM)—0.3482—pH 7.1, 37 °CPMID 10455188Open
hyaluronan synthase 3 (HAS3)Mus musculusUDP-alpha-D-glucuronic acidfixed UDP-GlcNAc at 0.1 mM; cosubstrate UDP-GlcNAc (0.1 mM)—0.0342—pH 7.1, 37 °CPMID 10455188Open
hyaluronan synthase 3 (HAS3)Mus musculusUDP-alpha-D-glucuronic acidfixed UDP-GlcNAc at 0.5 mM; cosubstrate UDP-GlcNAc (0.5 mM)—0.0351—pH 7.1, 37 °CPMID 10455188Open
hyaluronan synthase 3 (HAS3)Mus musculusUDP-alpha-D-glucuronic acidfixed UDP-GlcNAc at 2.0 mM; cosubstrate UDP-GlcNAc (2.0 mM)—0.0346—pH 7.1, 37 °CPMID 10455188Open
hyaluronan synthase 3 (HAS3)Mus musculusUDP-N-acetyl-alpha-D-glucosaminefixed UDP-GlcUA at 0.05 mM; cosubstrate UDP-GlcUA (0.05 mM)—0.0821—pH 7.1, 37 °CPMID 10455188Open
hyaluronan synthase 3 (HAS3)Mus musculusUDP-N-acetyl-alpha-D-glucosaminefixed UDP-GlcUA at 0.2 mM; cosubstrate UDP-GlcUA (0.2 mM)—0.2233—pH 7.1, 37 °CPMID 10455188Open
hyaluronan synthase 3 (HAS3)Mus musculusUDP-N-acetyl-alpha-D-glucosaminefixed UDP-GlcUA at 1.0 mM; cosubstrate UDP-GlcUA (1.0 mM)—0.2472—pH 7.1, 37 °CPMID 10455188Open