CatLogenzyme kinetics catalog

Values CatLog read from this paper

Kinetic characterization of the recombinant hyaluronan synthases from Streptococcus pyogenes and Streptococcus equisimilis

PMID 9933624doi:10.1074/jbc.274.7.4246Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 36 records (36 values) from this paper: hyaluronan synthase (seHAS), hyaluronan synthase (spHAS) from Streptococcus equisimilis, Streptococcus pyogenes.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.4.1.212

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 0.01 mM; in E. coli membranes—0.005—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 0.05 mM; in E. coli membranes—0.017—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 0.1 mM; in E. coli membranes—0.02—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 0.5 mM; in E. coli membranes—0.051—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 1.0 mM; in E. coli membranes—0.076—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 0.05 mM; 2 mM bovine cardiolipin—0.022—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 0.1 mM; 2 mM bovine cardiolipin—0.04—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 0.5 mM; 2 mM bovine cardiolipin—0.09—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 1.0 mM; 2 mM bovine cardiolipin—0.163—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 1.5 mM; 2 mM bovine cardiolipin—0.274—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidUDP-GlcNAc fixed at 2.0 mM; 2 mM bovine cardiolipin—0.37—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-glucuronic acidno added cardiolipin; 0.98 mM n-dodecyl beta-D-maltoside—0.054—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 0.01 mM; in E. coli membranes—0.009—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 0.05 mM; in E. coli membranes—0.019—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 0.1 mM; in E. coli membranes—0.03—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 0.5 mM; in E. coli membranes—0.06—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 1.0 mM; in E. coli membranes—0.074—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 0.01 mM; 2 mM bovine cardiolipin—0.018—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 0.05 mM; 2 mM bovine cardiolipin—0.041—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 0.1 mM; 2 mM bovine cardiolipin—0.062—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 0.5 mM; 2 mM bovine cardiolipin—0.164—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 1.0 mM; 2 mM bovine cardiolipin—0.251—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineUDP-GlcUA fixed at 1.5 mM; 2 mM bovine cardiolipin—0.329—pH 7, 30 °COpen
hyaluronan synthase (seHAS)Streptococcus equisimilisUDP-N-acetylglucosamineno added cardiolipin; 0.98 mM n-dodecyl beta-D-maltoside—1.06—pH 7, 30 °COpen
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 0.1 mM; in E. coli membranes—0.017—pH 7, 30 °COpen
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 0.2 mM; in E. coli membranes—0.025—pH 7, 30 °COpen
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 0.5 mM; in E. coli membranes—0.027—pH 7, 30 °COpen
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 1.0 mM; in E. coli membranes—0.035—pH 7, 30 °COpen
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 1.5 mM; in E. coli membranes—0.04—pH 7, 30 °COpen
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 0.1 mM; 2 mM bovine cardiolipin—0.02—pH 7, 30 °COpen
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 0.2 mM; 2 mM bovine cardiolipin—0.036—pH 7, 30 °COpen
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 0.5 mM; 2 mM bovine cardiolipin—0.066—pH 7, 30 °COpen
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 1.0 mM; 2 mM bovine cardiolipin—0.131—pH 7, 30 °COpen
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 1.5 mM; 2 mM bovine cardiolipin—0.175—pH 7, 30 °COpen
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidUDP-GlcNAc fixed at 2.0 mM; 2 mM bovine cardiolipin—0.216—pH 7, 30 °COpen
hyaluronan synthase (spHAS)Streptococcus pyogenesUDP-glucuronic acidno added cardiolipin; 0.98 mM n-dodecyl beta-D-maltoside—0.062—pH 7, 30 °COpen