Values CatLog read from this paper
Probing the catalytic mechanism of Escherichia coli amine oxidase using mutational variants and a reversible inhibitor as a substrate analogue
PMID 11985492doi:10.1042/bj20011435Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (22 values) from this paper: copper amine oxidase (ECAO) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 11 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| copper amine oxidase (ECAO) | Escherichia coli | 2-phenylethylamine | 9.6 | 0.00779 | 1.2 × 10³ calc. | pH 5.5, 25 °C | Open record: copper amine oxidase (ECAO), 2-phenylethylamine |
| copper amine oxidase (ECAO) | Escherichia coli | 2-phenylethylamine | 11.45 | 0.00228 | 5.02 × 10³ calc. | pH 5.75, 25 °C | Open record: copper amine oxidase (ECAO), 2-phenylethylamine |
| copper amine oxidase (ECAO) | Escherichia coli | 2-phenylethylamine | 20.7 | 0.00179 | 1.16 × 10⁴ calc. | pH 6, 25 °C | Open record: copper amine oxidase (ECAO), 2-phenylethylamine |
| copper amine oxidase (ECAO) | Escherichia coli | 2-phenylethylamine | 20.77 | 0.00169 | 1.23 × 10⁴ calc. | pH 6.5, 25 °C | Open record: copper amine oxidase (ECAO), 2-phenylethylamine |
| copper amine oxidase (ECAO) | Escherichia coli | 2-phenylethylamine | 14.98 | 0.0012 | 1.2 × 10⁴ calc. | pH 7, 25 °C | Open record: copper amine oxidase (ECAO), 2-phenylethylamine |
| copper amine oxidase (ECAO) | Escherichia coli | 2-phenylethylamine | 14.32 | 0.0017 | 8.4 × 10³ calc. | pH 7.5, 25 °C | Open record: copper amine oxidase (ECAO), 2-phenylethylamine |
| copper amine oxidase (ECAO) | Escherichia coli | 2-phenylethylamine | 13.68 | 0.00226 | 6.05 × 10³ calc. | pH 8, 25 °C | Open record: copper amine oxidase (ECAO), 2-phenylethylamine |
| copper amine oxidase (ECAO)variant D383E | Escherichia coli | 2-phenylethylamine | 0.01163 | 0.028 | 0.42 calc. | pH 5.5, 25 °C | Open record: copper amine oxidase (ECAO) variant D383E, 2-phenylethylamine |
| copper amine oxidase (ECAO)variant D383E | Escherichia coli | 2-phenylethylamine | 0.012 | 0.00962 | 1.3 calc. | pH 6, 25 °C | Open record: copper amine oxidase (ECAO) variant D383E, 2-phenylethylamine |
| copper amine oxidase (ECAO)variant D383E | Escherichia coli | 2-phenylethylamine | 0.00937 | 0.00247 | 3.79 calc. | pH 7, 25 °C | Open record: copper amine oxidase (ECAO) variant D383E, 2-phenylethylamine |
| copper amine oxidase (ECAO)variant D383E | Escherichia coli | 2-phenylethylamine | 0.00612 | 8.8 × 10⁻⁴ | 7.0 calc. | pH 8, 25 °C | Open record: copper amine oxidase (ECAO) variant D383E, 2-phenylethylamine |