Values CatLog read from this paper

Cold-adaptation mechanism of mutant enzymes of 3-isopropylmalate dehydrogenase from Thermus thermophilus

PMID 12082165doi:10.1093/protein/15.6.471Open in Browse

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CatLog read 28 records (74 values) from this paper: 3-isopropylmalate dehydrogenase from Thermus thermophilus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.85

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
3-isopropylmalate dehydrogenase	Thermus thermophilus	D,L-3-isopropylmalate	—	0.0044	4.55 × 10³	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase, D,L-3-isopropylmalate
3-isopropylmalate dehydrogenase	Thermus thermophilus	NAD+	20	0.166	121	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase, NAD+
3-isopropylmalate dehydrogenasevariant V126A	Thermus thermophilus	D,L-3-isopropylmalate	—	0.00256	3.5 × 10³	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126A, D,L-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant V126A	Thermus thermophilus	NAD+	8.97	0.0598	150	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126A, NAD+
3-isopropylmalate dehydrogenasevariant V126A	Thermus thermophilus	NAD+	1.23	0.00917	134	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant V126A, NAD+
3-isopropylmalate dehydrogenasevariant V126E	Thermus thermophilus	D,L-3-isopropylmalate	—	0.0061	3.38 × 10³	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126E, D,L-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant V126E	Thermus thermophilus	NAD+	20.6	0.109	189	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126E, NAD+
3-isopropylmalate dehydrogenasevariant V126E	Thermus thermophilus	NAD+	3.46	0.0515	67.1	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant V126E, NAD+
3-isopropylmalate dehydrogenasevariant V126F	Thermus thermophilus	D,L-3-isopropylmalate	—	0.0133	3.19 × 10³	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126F, D,L-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant V126F	Thermus thermophilus	NAD+	42.4	0.378	112	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126F, NAD+
3-isopropylmalate dehydrogenasevariant V126F	Thermus thermophilus	NAD+	10.9	0.144	75.9	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant V126F, NAD+
3-isopropylmalate dehydrogenasevariant V126G	Thermus thermophilus	D,L-3-isopropylmalate	—	0.00202	5.15 × 10³	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126G, D,L-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant V126G	Thermus thermophilus	NAD+	10.4	0.0725	144	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126G, NAD+
3-isopropylmalate dehydrogenasevariant V126G	Thermus thermophilus	NAD+	1.67	0.0239	69.7	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant V126G, NAD+
3-isopropylmalate dehydrogenasevariant V126I	Thermus thermophilus	D,L-3-isopropylmalate	—	0.00838	3.64 × 10³	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126I, D,L-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant V126I	Thermus thermophilus	NAD+	30.5	0.198	154	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126I, NAD+
3-isopropylmalate dehydrogenasevariant V126I	Thermus thermophilus	NAD+	6.05	0.0618	97.9	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant V126I, NAD+
3-isopropylmalate dehydrogenasevariant V126L	Thermus thermophilus	D,L-3-isopropylmalate	—	0.0121	3.68 × 10³	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126L, D,L-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant V126L	Thermus thermophilus	NAD+	44.5	0.391	114	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126L, NAD+
3-isopropylmalate dehydrogenasevariant V126L	Thermus thermophilus	NAD+	8.54	0.111	77.1	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant V126L, NAD+
3-isopropylmalate dehydrogenasevariant V126M	Thermus thermophilus	D,L-3-isopropylmalate	—	0.005	1.08 × 10⁴	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126M, D,L-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant V126M	Thermus thermophilus	NAD+	54.1	1.97	27.5	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126M, NAD+
3-isopropylmalate dehydrogenasevariant V126S	Thermus thermophilus	D,L-3-isopropylmalate	—	0.00324	3.92 × 10³	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126S, D,L-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant V126S	Thermus thermophilus	NAD+	12.7	0.0949	134	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126S, NAD+
3-isopropylmalate dehydrogenasevariant V126S	Thermus thermophilus	NAD+	1.49	0.00908	164	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant V126S, NAD+
3-isopropylmalate dehydrogenasevariant V126T	Thermus thermophilus	D,L-3-isopropylmalate	—	0.00268	5.49 × 10³	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126T, D,L-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant V126T	Thermus thermophilus	NAD+	14.7	0.103	143	pH 8, 60 °C	Open record: 3-isopropylmalate dehydrogenase variant V126T, NAD+
3-isopropylmalate dehydrogenasevariant V126T	Thermus thermophilus	NAD+	1.88	0.022	85.4	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant V126T, NAD+