Values CatLog read from this paper
Expression, purification, and characterization of two N,N-dimethyltransferases, tylM1 and desVI, involved in the biosynthesis of mycaminose and desosamine
PMID 12119032doi:10.1021/bi020245jOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 7 records (19 values) from this paper: TylM1 (N,N-dimethyltransferase), DesVI (N,N-dimethyltransferase) from Streptomyces fradiae, Streptomyces venezuelae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| DesVI (N,N-dimethyltransferase) | Streptomyces venezuelae | dTDP-3-amino-3,4,6-trideoxy-alpha-D-xylo-hexopyranose | 1.533 | 0.3074 | 4.988 | pH 7.5, 24 °C | Open record: DesVI (N,N-dimethyltransferase), dTDP-3-amino-3,4,6-trideoxy-alpha-D-xylo-hexopyranose |
| DesVI (N,N-dimethyltransferase) | Streptomyces venezuelae | dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose | 0.07 | 0.2766 | 0.253 | pH 7.5, 24 °C | Open record: DesVI (N,N-dimethyltransferase), dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose |
| TylM1 (N,N-dimethyltransferase) | Streptomyces fradiae | dTDP-3-(methylamino)-3,6-dideoxy-alpha-D-glucopyranose | 0.542 | 0.0468 | 11.57 | pH 7.5, 24 °C | Open record: TylM1 (N,N-dimethyltransferase), dTDP-3-(methylamino)-3,6-dideoxy-alpha-D-glucopyranose |
| TylM1 (N,N-dimethyltransferase) | Streptomyces fradiae | dTDP-3-amino-3,4,6-trideoxy-alpha-D-xylo-hexopyranose | 0.12 | 0.1184 | 1.013 | pH 7.5, 24 °C | Open record: TylM1 (N,N-dimethyltransferase), dTDP-3-amino-3,4,6-trideoxy-alpha-D-xylo-hexopyranose |
| TylM1 (N,N-dimethyltransferase) | Streptomyces fradiae | dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose | 0.165 | 0.0594 | 2.778 | pH 7.5, 24 °C | Open record: TylM1 (N,N-dimethyltransferase), dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose |
| TylM1 (N,N-dimethyltransferase) | Streptomyces fradiae | S-adenosyl-L-methionine1.28 mM 7 (saturating); 4.5 µM TylM1; first methylation step | 0.092 | 0.0989 | 0.93 calc. | pH 7.5, 24 °C | Open record: TylM1 (N,N-dimethyltransferase), S-adenosyl-L-methionine |
| TylM1 (N,N-dimethyltransferase) | Streptomyces fradiae | S-adenosyl-L-methionine0.89 mM 27; 0.56 µM TylM1; second methylation step | 0.857 | 0.117 | 7.32 calc. | pH 7.5, 24 °C | Open record: TylM1 (N,N-dimethyltransferase), S-adenosyl-L-methionine |