Values CatLog read from this paper
The active site loop of S-adenosylmethionine synthetase modulates catalytic efficiency
PMID 12135357doi:10.1021/bi025851tOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 31 records (55 values) from this paper: S-adenosylmethionine synthetase (EcMAT) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| S-adenosylmethionine synthetase (EcMAT) | Escherichia coli | adenosine 5'-triphosphate | — | 0.073 | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT), adenosine 5'-triphosphate |
| S-adenosylmethionine synthetase (EcMAT) | Escherichia coli | L-methioninecosubstrate ATP | 1.5 | — | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT), L-methionine |
| S-adenosylmethionine synthetase (EcMAT) | Escherichia coli | L-methioninecosubstrate ATP (saturating) | — | 0.092 | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT), L-methionine |
| S-adenosylmethionine synthetase (EcMAT) | Escherichia coli | L-methionine | 3 | — | — | pH 8, 37 °C | Open record: S-adenosylmethionine synthetase (EcMAT), L-methionine |
| S-adenosylmethionine synthetase (EcMAT) | Escherichia coli | tripolyphosphateno AdoMet | 0.06 | 0.003 | 20 | pH 7.8 | Open record: S-adenosylmethionine synthetase (EcMAT), tripolyphosphate |
| S-adenosylmethionine synthetase (EcMAT) | Escherichia coli | tripolyphosphatewith 100 µM AdoMet (activator) | 1.2 | 0.013 | 92 | pH 7.8 | Open record: S-adenosylmethionine synthetase (EcMAT), tripolyphosphate |
| S-adenosylmethionine synthetase (EcMAT)variant G5-EcMAT (loop DRADPLEQ replaced by GGGGG) | Escherichia coli | adenosine 5'-triphosphate | — | 0.087 | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT) variant G5-EcMAT (loop DRADPLEQ replaced by GGGGG), adenosine 5'-triphosphate |
| S-adenosylmethionine synthetase (EcMAT)variant G5-EcMAT (loop DRADPLEQ replaced by GGGGG) | Escherichia coli | L-methioninecosubstrate ATP | 0.0078 | — | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT) variant G5-EcMAT (loop DRADPLEQ replaced by GGGGG), L-methionine |
| S-adenosylmethionine synthetase (EcMAT)variant G5-EcMAT (loop DRADPLEQ replaced by GGGGG) | Escherichia coli | L-methioninecosubstrate ATP (saturating) | — | 0.77 | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT) variant G5-EcMAT (loop DRADPLEQ replaced by GGGGG), L-methionine |
| S-adenosylmethionine synthetase (EcMAT)variant G5-EcMAT (loop DRADPLEQ replaced by GGGGG) | Escherichia coli | tripolyphosphateno AdoMet | 0.0023 | 0.0053 | 0.43 | pH 7.8 | Open record: S-adenosylmethionine synthetase (EcMAT) variant G5-EcMAT (loop DRADPLEQ replaced by GGGGG), tripolyphosphate |
| S-adenosylmethionine synthetase (EcMAT)variant G5-EcMAT (loop DRADPLEQ replaced by GGGGG) | Escherichia coli | tripolyphosphatewith 100 µM AdoMet (activator) | 0.52 | 0.024 | 22 | pH 7.8 | Open record: S-adenosylmethionine synthetase (EcMAT) variant G5-EcMAT (loop DRADPLEQ replaced by GGGGG), tripolyphosphate |
| S-adenosylmethionine synthetase (EcMAT)variant G6-EcMAT (loop DRADPLEQ replaced by GGGGGG) | Escherichia coli | adenosine 5'-triphosphate | — | 0.026 | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT) variant G6-EcMAT (loop DRADPLEQ replaced by GGGGGG), adenosine 5'-triphosphate |
| S-adenosylmethionine synthetase (EcMAT)variant G6-EcMAT (loop DRADPLEQ replaced by GGGGGG) | Escherichia coli | L-methioninecosubstrate ATP | 0.0016 | — | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT) variant G6-EcMAT (loop DRADPLEQ replaced by GGGGGG), L-methionine |
| S-adenosylmethionine synthetase (EcMAT)variant G6-EcMAT (loop DRADPLEQ replaced by GGGGGG) | Escherichia coli | L-methioninecosubstrate ATP (saturating) | — | 0.3 | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT) variant G6-EcMAT (loop DRADPLEQ replaced by GGGGGG), L-methionine |
| S-adenosylmethionine synthetase (EcMAT)variant G6-EcMAT (loop DRADPLEQ replaced by GGGGGG) | Escherichia coli | tripolyphosphateno AdoMet | 0.022 | 0.0032 | 6.9 | pH 7.8 | Open record: S-adenosylmethionine synthetase (EcMAT) variant G6-EcMAT (loop DRADPLEQ replaced by GGGGGG), tripolyphosphate |
| S-adenosylmethionine synthetase (EcMAT)variant G6-EcMAT (loop DRADPLEQ replaced by GGGGGG) | Escherichia coli | tripolyphosphatewith 100 µM AdoMet (activator) | 0.84 | 0.011 | 76 | pH 7.8 | Open record: S-adenosylmethionine synthetase (EcMAT) variant G6-EcMAT (loop DRADPLEQ replaced by GGGGGG), tripolyphosphate |
| S-adenosylmethionine synthetase (EcMAT)variant G7-EcMAT (loop DRADPLEQ replaced by GGGGGGG) | Escherichia coli | adenosine 5'-triphosphate | — | 0.069 | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT) variant G7-EcMAT (loop DRADPLEQ replaced by GGGGGGG), adenosine 5'-triphosphate |
| S-adenosylmethionine synthetase (EcMAT)variant G7-EcMAT (loop DRADPLEQ replaced by GGGGGGG) | Escherichia coli | L-methioninecosubstrate ATP | 0.0043 | — | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT) variant G7-EcMAT (loop DRADPLEQ replaced by GGGGGGG), L-methionine |
| S-adenosylmethionine synthetase (EcMAT)variant G7-EcMAT (loop DRADPLEQ replaced by GGGGGGG) | Escherichia coli | L-methioninecosubstrate ATP (saturating) | — | 0.49 | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT) variant G7-EcMAT (loop DRADPLEQ replaced by GGGGGGG), L-methionine |
| S-adenosylmethionine synthetase (EcMAT)variant G7-EcMAT (loop DRADPLEQ replaced by GGGGGGG) | Escherichia coli | tripolyphosphateno AdoMet | 0.044 | 0.0015 | 29 | pH 7.8 | Open record: S-adenosylmethionine synthetase (EcMAT) variant G7-EcMAT (loop DRADPLEQ replaced by GGGGGGG), tripolyphosphate |
| S-adenosylmethionine synthetase (EcMAT)variant G7-EcMAT (loop DRADPLEQ replaced by GGGGGGG) | Escherichia coli | tripolyphosphatewith 100 µM AdoMet (activator) | 0.81 | 0.015 | 53 | pH 7.8 | Open record: S-adenosylmethionine synthetase (EcMAT) variant G7-EcMAT (loop DRADPLEQ replaced by GGGGGGG), tripolyphosphate |
| S-adenosylmethionine synthetase (EcMAT)variant G8-EcMAT (loop DRADPLEQ replaced by GGGGGGGG) | Escherichia coli | adenosine 5'-triphosphate | — | 0.045 | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT) variant G8-EcMAT (loop DRADPLEQ replaced by GGGGGGGG), adenosine 5'-triphosphate |
| S-adenosylmethionine synthetase (EcMAT)variant G8-EcMAT (loop DRADPLEQ replaced by GGGGGGGG) | Escherichia coli | L-methioninecosubstrate ATP | 0.0024 | — | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT) variant G8-EcMAT (loop DRADPLEQ replaced by GGGGGGGG), L-methionine |
| S-adenosylmethionine synthetase (EcMAT)variant G8-EcMAT (loop DRADPLEQ replaced by GGGGGGGG) | Escherichia coli | L-methioninecosubstrate ATP (saturating) | — | 0.62 | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT) variant G8-EcMAT (loop DRADPLEQ replaced by GGGGGGGG), L-methionine |
| S-adenosylmethionine synthetase (EcMAT)variant G8-EcMAT (loop DRADPLEQ replaced by GGGGGGGG) | Escherichia coli | tripolyphosphateno AdoMet | 0.079 | 0.0016 | 49 | pH 7.8 | Open record: S-adenosylmethionine synthetase (EcMAT) variant G8-EcMAT (loop DRADPLEQ replaced by GGGGGGGG), tripolyphosphate |
| S-adenosylmethionine synthetase (EcMAT)variant G8-EcMAT (loop DRADPLEQ replaced by GGGGGGGG) | Escherichia coli | tripolyphosphatewith 100 µM AdoMet (activator) | 1.2 | 0.008 | 150 | pH 7.8 | Open record: S-adenosylmethionine synthetase (EcMAT) variant G8-EcMAT (loop DRADPLEQ replaced by GGGGGGGG), tripolyphosphate |
| S-adenosylmethionine synthetase (EcMAT)variant RLL-EcMAT (loop DRADPLEQ replaced by rat liver MAT sequence HLDRNEEDV) | Escherichia coli | adenosine 5'-triphosphate | — | 0.045 | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT) variant RLL-EcMAT (loop DRADPLEQ replaced by rat liver MAT sequence HLDRNEEDV), adenosine 5'-triphosphate |
| S-adenosylmethionine synthetase (EcMAT)variant RLL-EcMAT (loop DRADPLEQ replaced by rat liver MAT sequence HLDRNEEDV) | Escherichia coli | L-methioninecosubstrate ATP | 0.0013 | — | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT) variant RLL-EcMAT (loop DRADPLEQ replaced by rat liver MAT sequence HLDRNEEDV), L-methionine |
| S-adenosylmethionine synthetase (EcMAT)variant RLL-EcMAT (loop DRADPLEQ replaced by rat liver MAT sequence HLDRNEEDV) | Escherichia coli | L-methioninecosubstrate ATP (saturating) | — | 0.23 | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (EcMAT) variant RLL-EcMAT (loop DRADPLEQ replaced by rat liver MAT sequence HLDRNEEDV), L-methionine |
| S-adenosylmethionine synthetase (EcMAT)variant RLL-EcMAT (loop DRADPLEQ replaced by rat liver MAT sequence HLDRNEEDV) | Escherichia coli | tripolyphosphateno AdoMet | 0.044 | 0.014 | 3.3 | pH 7.8 | Open record: S-adenosylmethionine synthetase (EcMAT) variant RLL-EcMAT (loop DRADPLEQ replaced by rat liver MAT sequence HLDRNEEDV), tripolyphosphate |
| S-adenosylmethionine synthetase (EcMAT)variant RLL-EcMAT (loop DRADPLEQ replaced by rat liver MAT sequence HLDRNEEDV) | Escherichia coli | tripolyphosphatewith 100 µM AdoMet (activator) | 0.78 | 0.026 | 30 | pH 7.8 | Open record: S-adenosylmethionine synthetase (EcMAT) variant RLL-EcMAT (loop DRADPLEQ replaced by rat liver MAT sequence HLDRNEEDV), tripolyphosphate |