Values CatLog read from this paper
Altering the substrate specificity of cephalosporin acylase by directed evolution of the Beta -subunit
PMID 12198140doi:10.1074/jbc.m208317200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (24 values) from this paper: glutaryl acylase (cephalosporin acylase) from Pseudomonas sp..
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 12 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glutaryl acylase (cephalosporin acylase) | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.46 | 0.96 | 0.48 calc. | pH 7.5, 37 °C | Open record: glutaryl acylase (cephalosporin acylase), adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase) | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 4.3 | 0.033 | 130 calc. | pH 7.5, 37 °C | Open record: glutaryl acylase (cephalosporin acylase), glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F229L | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.47 | 0.52 | 0.90 calc. | pH 7.5, 37 °C | Open record: glutaryl acylase (cephalosporin acylase) variant F229L, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F229L | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 3.1 | 0.048 | 65 calc. | pH 7.5, 37 °C | Open record: glutaryl acylase (cephalosporin acylase) variant F229L, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375L | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.67 | 0.82 | 0.82 calc. | pH 7.5, 37 °C | Open record: glutaryl acylase (cephalosporin acylase) variant F375L, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375L | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 1.1 | 0.27 | 4.1 calc. | pH 7.5, 37 °C | Open record: glutaryl acylase (cephalosporin acylase) variant F375L, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant M271V/Q291K/T374S | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.61 | 0.7 | 0.87 calc. | pH 7.5, 37 °C | Open record: glutaryl acylase (cephalosporin acylase) variant M271V/Q291K/T374S, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant M271V/Q291K/T374S | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 3.5 | 0.043 | 81 calc. | pH 7.5, 37 °C | Open record: glutaryl acylase (cephalosporin acylase) variant M271V/Q291K/T374S, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant N266H | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.55 | 0.14 | 3.9 calc. | pH 7.5, 37 °C | Open record: glutaryl acylase (cephalosporin acylase) variant N266H, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant N266H | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 3.3 | 0.016 | 210 calc. | pH 7.5, 37 °C | Open record: glutaryl acylase (cephalosporin acylase) variant N266H, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant N266S | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.34 | 0.42 | 0.81 calc. | pH 7.5, 37 °C | Open record: glutaryl acylase (cephalosporin acylase) variant N266S, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant N266S | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 2.2 | 0.094 | 23 calc. | pH 7.5, 37 °C | Open record: glutaryl acylase (cephalosporin acylase) variant N266S, glutaryl-7-aminocephalosporanic acid |