EC 3.5.1.93: glutaryl-7-aminocephalosporanic-acid acylase
HydrolasesOpen in Browse
CatLog read 124 records for EC 3.5.1.93 from 5 papers, for 2 organisms. Names in these papers: glutaryl acylase (cephalosporin acylase), glutaryl acylase, VAC glutaryl acylase, glutaryl acylase (cephalosporin acylase) SY-77 and 1 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 85 records for EC 3.5.1.93 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.5.1.93 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Analysis of a substrate specificity switch residue of cephalosporin acylase | Pseudomonas sp. | 38 |
| Mutational analysis of a key residue in the substrate specificity of a cephalosporin acylase | Pseudomonas sp. | 31 |
| doi:10.1016/j.molcatb.2011.12.001 | — | 26 |
| A highly active adipyl-cephalosporin acylase obtained via rational randomization | Pseudomonas | 17 |
| Altering the substrate specificity of cephalosporin acylase by directed evolution of the Beta -subunit | Pseudomonas sp. | 12 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| GAR glutaryl acylase | — | adipyl-7-aminocephalosporanic acid | — | 4.4 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: GAR glutaryl acylase, adipyl-7-aminocephalosporanic acid |
| GAR glutaryl acylase | — | adipyl-7-aminodesacetoxycephalosporanic acid | — | 3.8 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: GAR glutaryl acylase, adipyl-7-aminodesacetoxycephalosporanic acid |
| GAR glutaryl acylase | — | glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid | — | 2.2 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: GAR glutaryl acylase, glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid |
| GAR glutaryl acylase | — | glutaryl-7-aminocephalosporanic acid | — | 3.3 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: GAR glutaryl acylase, glutaryl-7-aminocephalosporanic acid |
| GAR glutaryl acylase | — | glutaryl-7-aminodesacetoxycephalosporanic acid | — | 1.5 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: GAR glutaryl acylase, glutaryl-7-aminodesacetoxycephalosporanic acid |
| GAR glutaryl acylase | — | glutaryl-7-Z-aminocephalosporanic acid | — | 5.4 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: GAR glutaryl acylase, glutaryl-7-Z-aminocephalosporanic acid |
| GAR glutaryl acylase | — | succinyl-7-aminocephalosporanic acid | — | 2.8 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: GAR glutaryl acylase, succinyl-7-aminocephalosporanic acid |
| glutaryl acylase | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.41 | 1.2 | 0.34 calc. | PMID 15174165 | Open record: glutaryl acylase, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 4 | 0.031 | 130 calc. | PMID 15174165 | Open record: glutaryl acylase, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266A | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.18 | 2.2 | 0.082 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266A, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266A | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 1.4 | 0.26 | 5.4 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266A, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266C | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.31 | 0.8 | 0.39 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266C, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266C | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 2 | 0.16 | 12 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266C, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266D | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.002 | 2.2 | 9.1 × 10⁻⁴ calc. | PMID 15174165 | Open record: glutaryl acylase variant N266D, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266E | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.023 | 13.8 | 0.0017 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266E, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266E | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.025 | 0.29 | 0.086 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266E, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266F | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.37 | 0.6 | 0.62 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266F, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266F | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.9 | 0.1 | 9.0 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266F, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266G | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.23 | 1.4 | 0.16 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266G, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266G | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 2 | 0.54 | 3.7 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266G, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266H | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.47 | 0.14 | 3.4 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266H, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266H | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 3.1 | 0.044 | 70 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266H, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266L | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.25 | 1 | 0.25 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266L, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266L | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.082 | 0.43 | 0.19 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266L, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266M | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 1.9 | 0.33 | 5.8 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266M, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266M | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 1.8 | 0.17 | 11 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266M, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266P | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.061 | 4.4 | 0.014 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266P, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266P | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.13 | 1.3 | 0.10 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266P, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266Q | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.46 | 0.62 | 0.74 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266Q, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266Q | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 2.4 | 0.12 | 20 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266Q, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266S | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.24 | 0.99 | 0.24 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266S, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266S | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 1.6 | 0.075 | 21 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266S, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266T | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.15 | 5.7 | 0.026 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266T, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266T | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.49 | 1.5 | 0.33 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266T, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266W | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.33 | 0.59 | 0.56 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266W, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266W | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 1.3 | 0.18 | 7.2 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266W, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266Y | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.33 | 0.65 | 0.51 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266Y, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266Y | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.61 | 0.11 | 5.5 calc. | PMID 15174165 | Open record: glutaryl acylase variant N266Y, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.46 | 0.96 | 0.48 calc. | pH 7.5, 37 °C | PMID 12198140 | Open record: glutaryl acylase (cephalosporin acylase), adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase) | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.49 | 1 | 0.49 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase), adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase) | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 4.3 | 0.033 | 130 calc. | pH 7.5, 37 °C | PMID 12198140 | Open record: glutaryl acylase (cephalosporin acylase), glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase) | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 4 | 0.031 | 130 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase), glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F229L | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.47 | 0.52 | 0.90 calc. | pH 7.5, 37 °C | PMID 12198140 | Open record: glutaryl acylase (cephalosporin acylase) variant F229L, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F229L | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 3.1 | 0.048 | 65 calc. | pH 7.5, 37 °C | PMID 12198140 | Open record: glutaryl acylase (cephalosporin acylase) variant F229L, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375A | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.2 | 0.51 | 0.39 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375A, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375A | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 2.35 | 0.07 | 34 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375A, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375C | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.55 | 0.17 | 3.2 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375C, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375C | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 2.308 | 0.038 | 61 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375C, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375D | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.015 | 11 | 0.0014 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375D, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375D | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.01 | 0.22 | 0.045 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375D, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375E | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.08 | 12 | 0.0067 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375E, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375E | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.008 | 0.2 | 0.040 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375E, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375G | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.073 | 0.16 | 0.46 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375G, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375G | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 2.46 | 0.066 | 37 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375G, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375H | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 1.16 | 0.9 | 1.3 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375H, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375H | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 1.334 | 0.11 | 12 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375H, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375I | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.097 | 2.07 | 0.047 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375I, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375I | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.67 | 0.1 | 6.7 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375I, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375K | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.036 | 2.16 | 0.017 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375K, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375K | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 2.2 | 0.7 | 3.1 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375K, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375L | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.67 | 0.82 | 0.82 calc. | pH 7.5, 37 °C | PMID 12198140 | Open record: glutaryl acylase (cephalosporin acylase) variant F375L, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375L | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.67 | 0.7 | 0.96 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375L, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375L | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 1.1 | 0.27 | 4.1 calc. | pH 7.5, 37 °C | PMID 12198140 | Open record: glutaryl acylase (cephalosporin acylase) variant F375L, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375L | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 1.1 | 0.26 | 4.2 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375L, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375M | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.65 | 0.65 | 1.0 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375M, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375M | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 2.02 | 0.17 | 12 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375M, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375N | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.9 | 0.83 | 1.1 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375N, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375N | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 1.96 | 0.18 | 11 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375N, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375Q | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.68 | 2.1 | 0.32 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375Q, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375Q | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.8 | 0.7 | 1.1 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375Q, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375R | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.018 | 10 | 0.0018 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375R, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375R | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.21 | 0.6 | 0.35 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375R, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375S | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.11 | 0.99 | 0.11 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375S, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375S | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 3.1 | 0.07 | 44 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375S, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375T | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.24 | 0.9 | 0.27 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375T, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375T | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 1.8 | 0.09 | 20 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375T, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375V | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.09 | 1.37 | 0.066 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375V, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375V | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 1.02 | 0.1 | 10 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375V, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375W | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.026 | 1.5 | 0.017 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375W, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375W | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.47 | 0.8 | 0.59 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375W, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375Y | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.43 | 0.7 | 0.61 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375Y, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant F375Y | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 3.64 | 0.08 | 46 calc. | pH 7.5, 37 °C | PMID 14680829 | Open record: glutaryl acylase (cephalosporin acylase) variant F375Y, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant M271V/Q291K/T374S | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.61 | 0.7 | 0.87 calc. | pH 7.5, 37 °C | PMID 12198140 | Open record: glutaryl acylase (cephalosporin acylase) variant M271V/Q291K/T374S, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant M271V/Q291K/T374S | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 3.5 | 0.043 | 81 calc. | pH 7.5, 37 °C | PMID 12198140 | Open record: glutaryl acylase (cephalosporin acylase) variant M271V/Q291K/T374S, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant N266H | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.55 | 0.14 | 3.9 calc. | pH 7.5, 37 °C | PMID 12198140 | Open record: glutaryl acylase (cephalosporin acylase) variant N266H, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant N266H | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 3.3 | 0.016 | 210 calc. | pH 7.5, 37 °C | PMID 12198140 | Open record: glutaryl acylase (cephalosporin acylase) variant N266H, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant N266S | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.34 | 0.42 | 0.81 calc. | pH 7.5, 37 °C | PMID 12198140 | Open record: glutaryl acylase (cephalosporin acylase) variant N266S, adipyl-7-aminodesacetoxycephalosporanic acid |
| glutaryl acylase (cephalosporin acylase)variant N266S | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 2.2 | 0.094 | 23 calc. | pH 7.5, 37 °C | PMID 12198140 | Open record: glutaryl acylase (cephalosporin acylase) variant N266S, glutaryl-7-aminocephalosporanic acid |
| glutaryl acylase (cephalosporin acylase) SY-77 | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.38 | 0.8 | 0.47 calc. | PMID 17922842 | Open record: glutaryl acylase (cephalosporin acylase) SY-77, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77 | Pseudomonas | glutaryl-7-aminocephalosporanic acid | 4.1 | 0.042 | 98 calc. | PMID 17922842 | Open record: glutaryl acylase (cephalosporin acylase) SY-77, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant N266Q/F375L | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.48 | 0.17 | 2.8 calc. | PMID 17922842 | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant N266Q/F375L, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant N266Q/F375L | Pseudomonas | glutaryl-7-aminocephalosporanic acid | 0.75 | 0.17 | 4.4 calc. | PMID 17922842 | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant N266Q/F375L, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.66 | 0.47 | 1.4 calc. | PMID 17922842 | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H | Pseudomonas | glutaryl-7-aminocephalosporanic acid | 2.5 | 0.08 | 31 calc. | PMID 17922842 | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/F229L | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.71 | 1.1 | 0.65 calc. | PMID 17922842 | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/F229L, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/F375H | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.44 | 0.56 | 0.79 calc. | PMID 17922842 | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/F375H, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/F375L | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 1 | 0.7 | 1.4 calc. | PMID 17922842 | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/F375L, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/M271V/Q291K/T374S | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.44 | 0.8 | 0.55 calc. | PMID 17922842 | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/M271V/Q291K/T374S, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/N266H | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.95 | 0.19 | 5.0 calc. | PMID 17922842 | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/N266H, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/N266H | Pseudomonas | glutaryl-7-aminocephalosporanic acid | 2.2 | 0.08 | 28 calc. | PMID 17922842 | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/N266H, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/N266M | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 1.2 | 0.17 | 7.1 calc. | PMID 17922842 | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/N266M, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/N266M | Pseudomonas | glutaryl-7-aminocephalosporanic acid | 0.89 | 0.3 | 3.0 calc. | PMID 17922842 | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/N266M, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/N266Q/F375M | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.72 | 0.28 | 2.6 calc. | PMID 17922842 | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/N266Q/F375M, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/N266Q/F375M | Pseudomonas | glutaryl-7-aminocephalosporanic acid | 0.72 | 0.62 | 1.2 calc. | PMID 17922842 | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/N266Q/F375M, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/N266S | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.43 | 0.9 | 0.48 calc. | PMID 17922842 | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/N266S, adipyl-7-aminodesacetoxycephalosporanic acid | |
| VAC glutaryl acylase | — | adipyl-7-aminocephalosporanic acid | — | 7.5 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase, adipyl-7-aminocephalosporanic acid |
| VAC glutaryl acylase | — | adipyl-7-aminodesacetoxycephalosporanic acidTable 2 | — | 0.7 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase, adipyl-7-aminodesacetoxycephalosporanic acid |
| VAC glutaryl acylase | — | adipyl-7-aminodesacetoxycephalosporanic acidin the text | 8.7 | — | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase, adipyl-7-aminodesacetoxycephalosporanic acid |
| VAC glutaryl acylase | — | cephalosporin C | — | 9.5 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase, cephalosporin C |
| VAC glutaryl acylase | — | glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid | — | 1 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase, glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid |
| VAC glutaryl acylase | — | glutaryl-7-aminocephalosporanic acid | — | 1.5 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase, glutaryl-7-aminocephalosporanic acid |
| VAC glutaryl acylase | — | glutaryl-7-aminodesacetoxycephalosporanic acid | — | 1.3 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase, glutaryl-7-aminodesacetoxycephalosporanic acid |
| VAC glutaryl acylase | — | glutaryl-7-Z-aminocephalosporanic acid | — | 2.5 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase, glutaryl-7-Z-aminocephalosporanic acid |
| VAC glutaryl acylase | — | pentanoyl-7-aminocephalosporanic acid | — | 17.4 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase, pentanoyl-7-aminocephalosporanic acid |
| VAC glutaryl acylase | — | succinyl-7-aminocephalosporanic acid | — | 2.8 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase, succinyl-7-aminocephalosporanic acid |
| VAC glutaryl acylasevariant H296S/H309S | — | adipyl-7-aminocephalosporanic acid | — | 4.3 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase variant H296S/H309S, adipyl-7-aminocephalosporanic acid |
| VAC glutaryl acylasevariant H296S/H309S | — | adipyl-7-aminodesacetoxycephalosporanic acid | — | 7.9 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase variant H296S/H309S, adipyl-7-aminodesacetoxycephalosporanic acid |
| VAC glutaryl acylasevariant H296S/H309S | — | cephalosporin C | — | 12.2 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase variant H296S/H309S, cephalosporin C |
| VAC glutaryl acylasevariant H296S/H309S | — | glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid | — | 8.7 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase variant H296S/H309S, glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid |
| VAC glutaryl acylasevariant H296S/H309S | — | glutaryl-7-aminocephalosporanic acid | — | 6.9 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase variant H296S/H309S, glutaryl-7-aminocephalosporanic acid |
| VAC glutaryl acylasevariant H296S/H309S | — | glutaryl-7-aminodesacetoxycephalosporanic acid | — | 14.1 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase variant H296S/H309S, glutaryl-7-aminodesacetoxycephalosporanic acid |
| VAC glutaryl acylasevariant H296S/H309S | — | glutaryl-7-Z-aminocephalosporanic acid | — | 3 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase variant H296S/H309S, glutaryl-7-Z-aminocephalosporanic acid |
| VAC glutaryl acylasevariant H296S/H309S | — | pentanoyl-7-aminocephalosporanic acid | — | 16.1 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase variant H296S/H309S, pentanoyl-7-aminocephalosporanic acid |
| VAC glutaryl acylasevariant H296S/H309S | — | succinyl-7-aminocephalosporanic acid | — | 5.4 | — | pH 8, 25 °C | doi:10.1016/j.molcatb.2011.12.001 | Open record: VAC glutaryl acylase variant H296S/H309S, succinyl-7-aminocephalosporanic acid |