CatLogenzyme kinetics catalog

Values CatLog read from this paper

Analysis of a substrate specificity switch residue of cephalosporin acylase

PMID 14680829doi:10.1016/j.bbrc.2003.10.180Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 38 records (76 values) from this paper: glutaryl acylase (cephalosporin acylase) from Pseudomonas sp..

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 38 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.5.1.93

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
glutaryl acylase (cephalosporin acylase)Pseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.4910.49 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)Pseudomonas sp.glutaryl-7-aminocephalosporanic acid40.031130 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375APseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.20.510.39 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375APseudomonas sp.glutaryl-7-aminocephalosporanic acid2.350.0734 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375CPseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.550.173.2 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375CPseudomonas sp.glutaryl-7-aminocephalosporanic acid2.3080.03861 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375DPseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.015110.0014 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375DPseudomonas sp.glutaryl-7-aminocephalosporanic acid0.010.220.045 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375EPseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.08120.0067 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375EPseudomonas sp.glutaryl-7-aminocephalosporanic acid0.0080.20.040 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375GPseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.0730.160.46 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375GPseudomonas sp.glutaryl-7-aminocephalosporanic acid2.460.06637 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375HPseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid1.160.91.3 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375HPseudomonas sp.glutaryl-7-aminocephalosporanic acid1.3340.1112 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375IPseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.0972.070.047 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375IPseudomonas sp.glutaryl-7-aminocephalosporanic acid0.670.16.7 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375KPseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.0362.160.017 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375KPseudomonas sp.glutaryl-7-aminocephalosporanic acid2.20.73.1 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375LPseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.670.70.96 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375LPseudomonas sp.glutaryl-7-aminocephalosporanic acid1.10.264.2 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375MPseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.650.651.0 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375MPseudomonas sp.glutaryl-7-aminocephalosporanic acid2.020.1712 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375NPseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.90.831.1 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375NPseudomonas sp.glutaryl-7-aminocephalosporanic acid1.960.1811 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375QPseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.682.10.32 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375QPseudomonas sp.glutaryl-7-aminocephalosporanic acid0.80.71.1 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375RPseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.018100.0018 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375RPseudomonas sp.glutaryl-7-aminocephalosporanic acid0.210.60.35 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375SPseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.110.990.11 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375SPseudomonas sp.glutaryl-7-aminocephalosporanic acid3.10.0744 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375TPseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.240.90.27 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375TPseudomonas sp.glutaryl-7-aminocephalosporanic acid1.80.0920 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375VPseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.091.370.066 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375VPseudomonas sp.glutaryl-7-aminocephalosporanic acid1.020.110 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375WPseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.0261.50.017 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375WPseudomonas sp.glutaryl-7-aminocephalosporanic acid0.470.80.59 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375YPseudomonas sp.adipyl-7-aminodesacetoxycephalosporanic acid0.430.70.61 calc.pH 7.5, 37 °COpen
glutaryl acylase (cephalosporin acylase)variant F375YPseudomonas sp.glutaryl-7-aminocephalosporanic acid3.640.0846 calc.pH 7.5, 37 °COpen